CNOT7 Polyclonal antibody

CNOT7 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14102-1-AP

Synonyms

BTG1 binding factor 1, CAF 1, CAF1, CCR4 associated factor 1, CNOT7, hCAF 1



Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

14102-1-AP targets CNOT7 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT7 fusion protein Ag5235
Full Name CCR4-NOT transcription complex, subunit 7
Calculated molecular weight 285 aa, 33 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC060852
Gene symbol CNOT7
Gene ID (NCBI) 29883
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cnot7, Ccr4-Not complex 7, is a component of CCR4-NOT complex and known to be a transcription factor and a modulator of mRNA degradation in yeast. Mammalian Cnot7 interacts with Tob and its family members, which is known as an inhibitor of BMP signaling through interfering with the Smad system and is involved in bone metabolism [PMID:17451368]. It is one of at least 8 subunits that make up the CCR4-Not transcription complex, which is involved in negative regulation of the pol II directed transcription in yeast24 and may be involved in global gene regulation by modulation of TATAA-binding protein (TBP) activity [PMID:10880468].

Protocols

Product Specific Protocols
WB protocol for CNOT7 antibody 14102-1-APDownload protocol
IP protocol for CNOT7 antibody 14102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cells

Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).

Authors - Tian-Li Duan

Int J Mol Med

B cell translocation gene 1 reduces the biological outcome of kidney cancer through induction of cell proliferation, cell cycle arrest, cell apoptosis and cell metastasis.

Authors - Guogui Sun
humanWB

Nature

DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.

Authors - Masashi Yamaji