|Positive WB detected in||mouse testis tissue|
|Positive IP detected in||mouse testis tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
14102-1-AP targets CNOT7 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CNOT7 fusion protein Ag5235|
|Full Name||CCR4-NOT transcription complex, subunit 7|
|Calculated molecular weight||285 aa, 33 kDa|
|Observed molecular weight||33-35 kDa|
|GenBank accession number||BC060852|
|Gene ID (NCBI)||29883|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cnot7, Ccr4-Not complex 7, is a component of CCR4-NOT complex and known to be a transcription factor and a modulator of mRNA degradation in yeast. Mammalian Cnot7 interacts with Tob and its family members, which is known as an inhibitor of BMP signaling through interfering with the Smad system and is involved in bone metabolism [PMID:17451368]. It is one of at least 8 subunits that make up the CCR4-Not transcription complex, which is involved in negative regulation of the pol II directed transcription in yeast24 and may be involved in global gene regulation by modulation of TATAA-binding protein (TBP) activity [PMID:10880468].
Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).
Int J Mol Med
B cell translocation gene 1 reduces the biological outcome of kidney cancer through induction of cell proliferation, cell cycle arrest, cell apoptosis and cell metastasis.
DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs.