COA4 Recombinant antibody

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  WB analysis using 83326-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83326-1-RR (COA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovarian cancer using 83326-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83326-1-RR (COA4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovarian cancer using 83326-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83326-1-RR (COA4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 83326-1-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83326-1-RR (COA4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83326-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83326-1-RR against Human COA4 were performed. The affinity constant is 0.307 nM.

COA4 Recombinant Antibody for WB, IHC, ELISA

Cat No. 83326-1-RR

Clone No.230070G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CHCHD8, E2IG2, E2 induced gene 2 protein, 230070G9

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, K-562 cells, HEK-293 cells, PC-3 cells, LNCaP cells, MKN-45 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human ovarian cancer; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83326-1-RR targets COA4 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: COA4 fusion protein Ag33966
• Full Name: Cytochrome c oxidase assembly factor 4 homolog, mitochondrial
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: NM_016565.2
• Gene Symbol: COA4
• Gene ID (NCBI): 51287
• RRID: AB_3670991
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NYJ1-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.