COG4 Polyclonal antibody

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  WB analysis using 31539-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31539-1-AP (COG4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 31539-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31539-1-AP (COG4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 31539-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31539-1-AP (COG4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COG4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31539-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

COD1, COG complex subunit 4

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Tested Applications

• Positive WB detected in: HT-1080 cells, mouse spermatophore tissue, MRC-5 cells, hTERT-RPE1 cells, mouse testis tisuue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31539-1-AP targets COG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COG4 fusion protein Ag35295
• Full Name: component of oligomeric golgi complex 4
• Calculated Molecular Weight: 89kDa，785aa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: NM_001365426.1
• Gene Symbol: COG4
• Gene ID (NCBI): 25839
• RRID: AB_3670028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H9E3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COG complex subunit 4 is present in high molecular weight complexes that govern membrane movement between the Golgi and ER. Cog4 dependent trafficking and secretion of ECM components are required for protrusion and extension of the epithelial projections of the inner ear (PMID: 30287385). Loss-of-function COG4-mutant cells have impaired glycosylation and reduced COG4 protein levels (PMID: 30290151).