COG8 Polyclonal antibody, PBS Only

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  WB analysis using 12661-1-AP (same clone as 12661-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12661-1-AP (COG8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12661-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 12661-1-AP (same clone as 12661-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12661-1-AP (COG8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12661-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 12661-1-AP (same clone as 12661-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12661-1-AP (COG8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12661-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 12661-1-AP (same clone as 12661-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using COG8 antibody (12661-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12661-1-PBS in a different storage buffer formulation.

COG8 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 12661-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

COG complex subunit 8, DOR1

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Product Information

12661-1-PBS targets COG8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COG8 fusion protein Ag3341
• Full Name: component of oligomeric golgi complex 8
• Calculated Molecular Weight: 612 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC017492
• Gene Symbol: COG8
• Gene ID (NCBI): 84342
• RRID: AB_2244993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96MW5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The conserved oligomeric Golgi (COG) complex is an eight subunit (COG1-8) peripheral Golgi membrane hetero-oligomeric protein complex. The COG complex is organized into lobes A (COG2-4) and B (COG5-7) with COG1 and COG8 bridging these lobes and is thought to play a critical role in vesicle tethering processes (PMID: 17331980). Cog8 localizes exclusively in the Golgi apparatus and is involved in the retrograde Golgi transport of resident proteins responsible for sugar chain (glycan) biosynthesis (PMID: 17220172).