Tested Applications
| Positive WB detected in | mouse liver tissue, human plasma |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | ATCD5 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15269-1-AP targets COLEC11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7374 Product name: Recombinant human COLEC11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 18-271 aa of BC000078 Sequence: SLLPSGHPQPAGDDACSVQILVPGLKGDAGEKGDKGAPGRPGRVGPTGEKGDMGDKGQKGSVGRHGKIGPIGSKGEKGDSGDIGPPGPNGEPGLPCECSQLRKAIGEMDNQVSQLTSELKFIKNAVAGVRETESKIYLLVKEEKRYADAQLSCQGRGGTLSMPKDEAANGLMAAYLAQAGLARVFIGINDLEKEGAFVYSDHSPMRTFNKWRSGEPNNAYDEEDCVEMVASGGWNDVACHTTMYFMCEFDKENM Predict reactive species |
| Full Name | collectin sub-family member 11 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC000078 |
| Gene Symbol | COLEC11 |
| Gene ID (NCBI) | 78989 |
| RRID | AB_10732940 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BWP8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COLEC11, also named as CL-K1 and Collectin-11, belongs to the COLEC10/COLEC11 family. It is a lectin that binds to various sugars: fucose > mannose. It does not bind to glucose, N-acetylglucosamine and N-acetylgalactosamine. COLEC11 binds to LPS. COLEC11 and MASP1 are two genes in the lectin complement pathway, they are mutated in 3MC syndrome, implicating this diverse inflammation-chemotaxis cascade in the etiology of human developmental disorders. COLEC11 serves as a guidance cue for neural crest cell migration.(PMID:21258343)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for COLEC11 antibody 15269-1-AP | Download protocol |
| IP protocol for COLEC11 antibody 15269-1-AP | Download protocol |
| WB protocol for COLEC11 antibody 15269-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Collectin-11 (CL-11) antibody (Cat# 15269-1-AP) has 8 total citations. These appear in Nature Genetics, Clinical Journal of the American Society of Nephrology, Frontiers in Immunology, Journal of Innate Immunity, FASEB Journal, Clinical Proteomics, and Journal of Immunology. Research fields span the lectin complement pathway, IgA nephropathy, colorectal cancer metastasis, and diabetic nephropathy biomarker discovery.
| Species | Application | Title |
|---|---|---|
Nat Genet Mutations in lectin complement pathway genes COLEC11 and MASP1 cause 3MC syndrome. | ||
Front Immunol ANGPTL2+cancer-associated fibroblasts and SPP1+macrophages are metastasis accelerators of colorectal cancer | ||
Front Immunol Development of a Quantitative Assay for the Characterization of Human Collectin-11 (CL-11, CL-K1). | ||
J Innate Immun Collectin-11/MASP Complex Formation Triggers Activation of the Lectin Complement Pathway - The Fifth Lectin Pathway Initiation Complex. | ||
Reviews
What customers say
Users report consistent detection of recombinant CL-11 in Western blot, immunohistochemistry, and immunofluorescence at 1:1000 (WB) and 1:100 (IHC/IF). However, both reviewers noted off-target binding: multiple non-specific bands appear in cell lysates, and the antibody shows cross-reactivity in CL-11 knockout mice. Overall, the antibody performs reliably for recombinant protein detection but may require optimization or additional controls when used with complex lysates or tissue samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Christopher (Verified Customer) (08-28-2019) | Antibody works for identification of recombinant CL-11 well however multiple bands appears on cell lysates.
|
mark (Verified Customer) (08-13-2019) | Consitent fro WB but binds non CL-11 in cell lysatesFor immunohistochemistry/immunoflourescence binds CL-11 consistently but also has off target effects (eg in CL-11 KO mice)
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