COLEC11 Polyclonal antibody

COLEC11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15269-1-AP

Synonyms

Collectin-11, UNQ596/PRO1182, Collectin 11, CL-K1, CLK1



Tested Applications

Positive WB detected inmouse liver tissue, human plasma
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inATCD5 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15269-1-AP targets COLEC11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COLEC11 fusion protein Ag7374
Full Name collectin sub-family member 11
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000078
Gene Symbol COLEC11
Gene ID (NCBI) 78989
RRIDAB_10732940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COLEC11, also named as CL-K1 and Collectin-11, belongs to the COLEC10/COLEC11 family. It is a lectin that binds to various sugars: fucose > mannose. It does not bind to glucose, N-acetylglucosamine and N-acetylgalactosamine. COLEC11 binds to LPS. COLEC11 and MASP1 are two genes in the lectin complement pathway, they are mutated in 3MC syndrome, implicating this diverse inflammation-chemotaxis cascade in the etiology of human developmental disorders. COLEC11 serves as a guidance cue for neural crest cell migration.(PMID:21258343)

Protocols

Product Specific Protocols
WB protocol for COLEC11 antibody 15269-1-APDownload protocol
IHC protocol for COLEC11 antibody 15269-1-APDownload protocol
IP protocol for COLEC11 antibody 15269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC,IF

Nat Genet

Mutations in lectin complement pathway genes COLEC11 and MASP1 cause 3MC syndrome.

Authors - Rooryck Caroline C
humanWB,IF

Clin J Am Soc Nephrol

Collectin11 and Complement Activation in IgA Nephropathy.

Authors - Min Wei
mouseWB

Front Immunol

Development of a Quantitative Assay for the Characterization of Human Collectin-11 (CL-11, CL-K1).

Authors - Rafael Bayarri-Olmos
humanWB

J Immunol

Heterocomplex Formation between MBL/Ficolin/CL-11-Associated Serine Protease-1 and -3 and MBL/Ficolin/CL-11-Associated Protein-1.

Authors - Anne Rosbjerg
humanFC

J Innate Immun

Collectin-11/MASP Complex Formation Triggers Activation of the Lectin Complement Pathway - The Fifth Lectin Pathway Initiation Complex.

Authors - Ma Ying Jie YJ
humanWB

Clin Proteomics

iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.

Authors - Hongmei Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Christopher (Verified Customer) (08-28-2019)

Antibody works for identification of recombinant CL-11 well however multiple bands appears on cell lysates.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human PTECs
FH

mark (Verified Customer) (08-13-2019)

Consitent fro WB but binds non CL-11 in cell lysatesFor immunohistochemistry/immunoflourescence binds CL-11 consistently but also has off target effects (eg in CL-11 KO mice)

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 for WB, 1:100 for Immuno
  • Cell Tissue Type: mouse cell culture, mouse kidney, mouse embryos, human kidney