Validation Data Gallery
|Positive WB detected in||mouse liver tissue, human plasma|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||mouse liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||ATCD5 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15269-1-AP targets COLEC11 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COLEC11 fusion protein Ag7374|
|Full Name||collectin sub-family member 11|
|Calculated molecular weight||29 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC000078|
|Gene ID (NCBI)||78989|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
COLEC11, also named as CL-K1 and Collectin-11, belongs to the COLEC10/COLEC11 family. It is a lectin that binds to various sugars: fucose > mannose. It does not bind to glucose, N-acetylglucosamine and N-acetylgalactosamine. COLEC11 binds to LPS. COLEC11 and MASP1 are two genes in the lectin complement pathway, they are mutated in 3MC syndrome, implicating this diverse inflammation-chemotaxis cascade in the etiology of human developmental disorders. COLEC11 serves as a guidance cue for neural crest cell migration.(PMID:21258343)
Mutations in lectin complement pathway genes COLEC11 and MASP1 cause 3MC syndrome.
Clin J Am Soc Nephrol
Collectin11 and Complement Activation in IgA Nephropathy.
Development of a Quantitative Assay for the Characterization of Human Collectin-11 (CL-11, CL-K1).
Heterocomplex Formation between MBL/Ficolin/CL-11-Associated Serine Protease-1 and -3 and MBL/Ficolin/CL-11-Associated Protein-1.
J Innate Immun
Collectin-11/MASP Complex Formation Triggers Activation of the Lectin Complement Pathway - The Fifth Lectin Pathway Initiation Complex.
iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Christopher (Verified Customer) (08-28-2019)
Antibody works for identification of recombinant CL-11 well however multiple bands appears on cell lysates.
mark (Verified Customer) (08-13-2019)
Consitent fro WB but binds non CL-11 in cell lysatesFor immunohistochemistry/immunoflourescence binds CL-11 consistently but also has off target effects (eg in CL-11 KO mice)