COLEC11 Polyclonal antibody

COLEC11 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15269-1-AP

Synonyms

CL K1, CL K1 I, CL K1 II, CL K1 IIa, CL K1 IIb, CLK1, COLEC11, Collectin 11, Collectin kidney protein 1, collectin sub family member 11



Tested Applications

Positive WB detected inmouse liver tissue, human plasma tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inATCD5 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15269-1-AP targets COLEC11 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COLEC11 fusion protein Ag7374
Full Name collectin sub-family member 11
Calculated molecular weight 29 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC000078
Gene symbol COLEC11
Gene ID (NCBI) 78989
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COLEC11, also named as CL-K1 and Collectin-11, belongs to the COLEC10/COLEC11 family. It is a lectin that binds to various sugars: fucose > mannose. It does not bind to glucose, N-acetylglucosamine and N-acetylgalactosamine. COLEC11 binds to LPS. COLEC11 and MASP1 are two genes in the lectin complement pathway, they are mutated in 3MC syndrome, implicating this diverse inflammation-chemotaxis cascade in the etiology of human developmental disorders. COLEC11 serves as a guidance cue for neural crest cell migration.(PMID:21258343)

Protocols

Product Specific Protocols
WB protocol for COLEC11 antibody 15269-1-APDownload protocol
IHC protocol for COLEC11 antibody 15269-1-APDownload protocol
IP protocol for COLEC11 antibody 15269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Proteomics

iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.

Authors - Hongmei Lu
humanFC

J Innate Immun

Collectin-11/MASP Complex Formation Triggers Activation of the Lectin Complement Pathway - The Fifth Lectin Pathway Initiation Complex.

Authors - Ma Ying Jie YJ
humanWB

J Immunol

Heterocomplex Formation between MBL/Ficolin/CL-11-Associated Serine Protease-1 and -3 and MBL/Ficolin/CL-11-Associated Protein-1.

Authors - Anne Rosbjerg
mouseWB

Front Immunol

Development of a Quantitative Assay for the Characterization of Human Collectin-11 (CL-11, CL-K1).

Authors - Rafael Bayarri-Olmos
mouseIHC,IF

Nat Genet

Mutations in lectin complement pathway genes COLEC11 and MASP1 cause 3MC syndrome.

Authors - Rooryck Caroline C

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christopher (Verified Customer) (08-28-2019)

Antibody works for identification of recombinant CL-11 well however multiple bands appears on cell lysates.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human PTECs
FH

mark (Verified Customer) (08-13-2019)

Consitent fro WB but binds non CL-11 in cell lysates For immunohistochemistry/immunoflourescence binds CL-11 consistently but also has off target effects (eg in CL-11 KO mice)

  • Applications: Western Blot,Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 for WB, 1:100 for Immuno
  • Cell Tissue Type: mouse cell culture, mouse kidney, mouse embryos, human kidney