|Positive WB detected in||HeLa cells, mouse skeletal muscle tissue, human brain tissue, HEK-293T cells, mouse brain tissue, NIH/3T3 cells|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10969-2-AP targets COPS2/TRIP15 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||bovine, human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||COPS2/TRIP15 fusion protein Ag1415|
|Full Name||COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)|
|Calculated molecular weight||52 kDa|
|Observed molecular weight||52 kDa|
|GenBank accession number||BC012629|
|Gene ID (NCBI)||9318|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. (refer to UniProt) Catalog#10969-2-AP is a rabbit polyclonal antibody raised against the full-length of human COPS2. The MW of this protein is 52 kDa, and this antibody specially recognises the 52 kDa protein.
|Product Specific Protocols|
|WB protocol for COPS2/TRIP15 antibody 10969-2-AP||Download protocol|
|IHC protocol for COPS2/TRIP15 antibody 10969-2-AP||Download protocol|
|IF protocol for COPS2/TRIP15 antibody 10969-2-AP||Download protocol|
|IP protocol for COPS2/TRIP15 antibody 10969-2-AP||Download protocol|
|Click here to view our Standard Protocols|
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