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  • KD/KO Validated

CSN2 Polyclonal antibody

CSN2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 10969-2-AP


ALIEN, Alien homolog, COPS 2, COPS2, COPS2/TRIP15, CSN2, SGN2, Signalosome subunit 2, TR interacting protein 15, TRIP 15, TRIP15

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, HEK-293 cells, mouse brain tissue, NIH/3T3 cells, mouse kidney tissue
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10969-2-AP targets CSN2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSN2 fusion protein Ag1415
Full Name COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC012629
Gene Symbol COPS2
Gene ID (NCBI) 9318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. (refer to UniProt) Catalog#10969-2-AP is a rabbit polyclonal antibody raised against the full-length of human COPS2. The MW of this protein is 52 kDa, and this antibody specially recognises the 52 kDa protein.


Product Specific Protocols
WB protocol for CSN2 antibody 10969-2-APDownload protocol
IHC protocol for CSN2 antibody 10969-2-APDownload protocol
IF protocol for CSN2 antibody 10969-2-APDownload protocol
IP protocol for CSN2 antibody 10969-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

Glucose-induced CRL4COP1-p53 axis amplifies glycometabolism to drive tumorigenesis

Authors - Yang Su

Nat Commun

IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion.

Authors - Hong Lin
  • KD Validated

Proc Natl Acad Sci U S A

Inositol hexakisphosphate kinase-1 mediates assembly/disassembly of the CRL4-signalosome complex to regulate DNA repair and cell death.

Authors - Feng Rao

Proc Natl Acad Sci U S A

Inositol hexakisphosphate (IP6) generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function.

Authors - Paul C Scherer

Proc Natl Acad Sci U S A

Basis for metabolite-dependent Cullin-RING ligase deneddylation by the COP9 signalosome.

Authors - Hong Lin


Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia

Authors - Yu-Han H Hsu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Resha (Verified Customer) (11-01-2023)

In 20ug of total protein, 1:1000 dilution of anti-COPS2 antibody gave clear band of 52kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa cells