COPS2/TRIP15 Polyclonal antibody

COPS2/TRIP15 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10969-2-AP

Synonyms

ALIEN, Alien homolog, COPS 2, COPS2, CSN2, SGN2, Signalosome subunit 2, TR interacting protein 15, TRIP 15, TRIP15

---

Validation Data Gallery

• 

  WB analysis using 10969-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10969-2-AP (COPS2/TRIP15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 10969-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10969-2-AP (COPS2/TRIP15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10969-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10969-2-AP (COPS2/TRIP15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IP experiment of NIH/3T3 using 10969-2-AP

  IP Result of anti-COPS2/TRIP15 (IP:10969-2-AP, 3ug; Detection:10969-2-AP 1:500) with NIH/3T3 cells lysate 2500ug.

• 

  WB analysis of HeLa using 10969-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10969-2-AP (COPS2/TRIP15 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 10969-2-AP

  Immunofluorescent analysis of HeLa cells using 10969-2-AP (COPS2/TRIP15 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse skeletal muscle tissue, human brain tissue, HEK-293T cells, mouse brain tissue, NIH/3T3 cells
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

10969-2-AP targets COPS2/TRIP15 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: bovine, human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COPS2/TRIP15 fusion protein Ag1415
• Full Name: COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
• Calculated molecular weight: 52 kDa
• Observed molecular weight: 52 kDa
• GenBank accession number: BC012629
• Gene symbol: COPS2
• Gene ID (NCBI): 9318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. (refer to UniProt) Catalog#10969-2-AP is a rabbit polyclonal antibody raised against the full-length of human COPS2. The MW of this protein is 52 kDa, and this antibody specially recognises the 52 kDa protein.

Publications

Species	Application	Title
human	IHC	Oncotarget; RMP promotes epithelial-mesenchymal transition through NF-κB/CSN2/Snail pathway in hepatocellular carcinoma.; Authors - Wei Zhou
bovine	WB	Int J Mol Sci; Kisspeptin-10 Induces β-Casein Synthesis via GPR54 and Its Downstream Signaling Pathways in Bovine Mammary Epithelial Cells.; Authors - Jianhua Sun
human	WB	Proc Natl Acad Sci U S A; Basis for metabolite-dependent Cullin-RING ligase deneddylation by the COP9 signalosome.; Authors - Hong Lin
human	WB	Proc Natl Acad Sci U S A; Inositol hexakisphosphate (IP6) generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function.; Authors - Paul C Scherer
human	WB	Proc Natl Acad Sci U S A; Inositol hexakisphosphate kinase-1 mediates assembly/disassembly of the CRL4-signalosome complex to regulate DNA repair and cell death.; Authors - Feng Rao