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COPS2/TRIP15 Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 10969-2-AP

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Con: 74 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

COPS2/TRIP15 Antibody WB HEK-293T cells 10969-2-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10969-2-AP (COPS2/TRIP15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

COPS2/TRIP15 Antibody IHC human ovary tumor tissue 10969-2-AP

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10969-2-AP (COPS2/TRIP15 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

COPS2/TRIP15 Antibody IHC human ovary tumor tissue 10969-2-AP

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10969-2-AP (COPS2/TRIP15 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

COPS2/TRIP15 Antibody IP NIH/3T3 cells 10969-2-AP

IP Result of anti-COPS2/TRIP15 (IP:10969-2-AP, 3ug; Detection:10969-2-AP 1:500) with NIH/3T3 cells lysate 2500ug.

COPS2/TRIP15 Antibody WB HeLa cells 10969-2-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 10969-2-AP( COPS2/TRIP15 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

COPS2/TRIP15 Antibody IF HeLa cells 10969-2-AP

Immunofluorescent analysis of HeLa cells using 10969-2-AP(COPS2/TRIP15 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, cow
Positive WB detected in:
HeLa cells, mouse skeletal muscle tissue, human brain tissue, HEK-293T cells, mouse brain tissue, NIH/3T3 cells
Positive IP detected in:
NIH/3T3 cells
Positive IHC detected in:
human ovary tumor tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
COPS2/TRIP15 fusion protein Ag1415
Full name:
COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
Calculated molecular weight:
52 kDa
Observed molecular weight:
52 kDa
GenBank accession number:
BC012629
Gene ID (NCBI):
9318
Gene symbol
COPS2
Synonyms
ALIEN, Alien homolog, COPS 2, COPS2, CSN2, SGN2, Signalosome subunit 2, TR interacting protein 15, TRIP 15, TRIP15

Background

COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. (refer to UniProt) Catalog#10969-2-AP is a rabbit polyclonal antibody raised against the full-length of human COPS2. The MW of this protein is 52 kDa, and this antibody specially recognises the 52 kDa protein.


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