CSN2 Polyclonal antibody

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  WB analysis using 10969-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10969-2-AP (CSN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10969-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10969-2-AP (CSN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10969-2-AP

  WB result of CSN2 antibody (10969-2-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSN2 transfected HEK-293 cells.

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  WB analysis of NIH/3T3 using 10969-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10969-2-AP (CSN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10969-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10969-2-AP (CSN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 10969-2-AP

  IP result of anti-CSN2 (IP:10969-2-AP, 3ug; Detection:10969-2-AP 1:500) with NIH/3T3 cells lysate 2500ug.

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  IHC staining of human ovary tumor using 10969-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10969-2-AP (CSN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10969-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10969-2-AP (CSN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10969-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CSN2 antibody (10969-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

CSN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10969-2-AP

Publications(10)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

COPS2, JAB1-containing signalosome subunit 2, COPS2/TRIP15, COPS 2, Alien homolog

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, HEK-293 cells, mouse brain tissue, NIH/3T3 cells, mouse kidney tissue
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10969-2-AP targets CSN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSN2 fusion protein Ag1415
• Full Name: COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC012629
• Gene Symbol: COPS2
• Gene ID (NCBI): 9318
• RRID: AB_2276346
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61201
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. (refer to UniProt) Catalog#10969-2-AP is a rabbit polyclonal antibody raised against the full-length of human COPS2. The MW of this protein is 52 kDa, and this antibody specially recognises the 52 kDa protein.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; Glucose-induced CRL4COP1-p53 axis amplifies glycometabolism to drive tumorigenesis; Authors - Yang Su
mouse	WB	Nat Commun; IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion.; Authors - Hong Lin ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Inositol hexakisphosphate kinase-1 mediates assembly/disassembly of the CRL4-signalosome complex to regulate DNA repair and cell death.; Authors - Feng Rao
human	WB	Proc Natl Acad Sci U S A; Inositol hexakisphosphate (IP6) generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function.; Authors - Paul C Scherer
human	WB	Proc Natl Acad Sci U S A; Basis for metabolite-dependent Cullin-RING ligase deneddylation by the COP9 signalosome.; Authors - Hong Lin
human	WB	iScience; Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia; Authors - Yu-Han H Hsu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Resha (Verified Customer) (11-01-2023)

In 20ug of total protein, 1:1000 dilution of anti-COPS2 antibody gave clear band of 52kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells