COPS2/TRIP15 Polyclonal antibody

COPS2/TRIP15 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10969-2-AP

Synonyms

ALIEN, Alien homolog, COPS 2, COPS2, CSN2, SGN2, Signalosome subunit 2, TR interacting protein 15, TRIP 15, TRIP15



Tested Applications

Positive WB detected inHeLa cells, mouse skeletal muscle tissue, human brain tissue, HEK-293T cells, mouse brain tissue, NIH/3T3 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10969-2-AP targets COPS2/TRIP15 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity cow, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COPS2/TRIP15 fusion protein Ag1415
Full Name COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC012629
Gene symbol COPS2
Gene ID (NCBI) 9318
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. (refer to UniProt) Catalog#10969-2-AP is a rabbit polyclonal antibody raised against the full-length of human COPS2. The MW of this protein is 52 kDa, and this antibody specially recognises the 52 kDa protein.

Protocols

Product Specific Protocols
WB protocol for COPS2/TRIP15 antibody 10969-2-APDownload protocol
IHC protocol for COPS2/TRIP15 antibody 10969-2-APDownload protocol
IF protocol for COPS2/TRIP15 antibody 10969-2-APDownload protocol
IP protocol for COPS2/TRIP15 antibody 10969-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncotarget

RMP promotes epithelial-mesenchymal transition through NF-κB/CSN2/Snail pathway in hepatocellular carcinoma.

Authors - Wei Zhou
cowWB

Int J Mol Sci

Kisspeptin-10 Induces β-Casein Synthesis via GPR54 and Its Downstream Signaling Pathways in Bovine Mammary Epithelial Cells.

Authors - Jianhua Sun
humanWB

Proc Natl Acad Sci U S A

Inositol hexakisphosphate (IP6) generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function.

Authors - Paul C Scherer
humanWB

Proc Natl Acad Sci U S A

Inositol hexakisphosphate kinase-1 mediates assembly/disassembly of the CRL4-signalosome complex to regulate DNA repair and cell death.

Authors - Feng Rao