CORO7 Polyclonal antibody

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  WB analysis using 31425-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31425-1-AP (CORO7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 31425-1-AP

  IP result of anti-CORO7 (IP:31425-1-AP, 4ug; Detection:31425-1-AP 1:2000) with HeLa cells lysate 980 ug.

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  IHC staining of human ovary cancer using 31425-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31425-1-AP (CORO7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CORO7 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 31425-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

CRN7, Coronin-7

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells, MDA-MB-231 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31425-1-AP targets CORO7 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CORO7 fusion protein Ag35016
• Full Name: coronin 7
• Calculated Molecular Weight: 101 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_024535
• Gene Symbol: CORO7
• Gene ID (NCBI): 79585
• RRID: AB_3669976
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P57737
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CORO7 also known as CRN7 belongs to the WD repeat coronin family, and functions as an F-actin regulator directing anterograde Golgi to endosome transport(PMID: 15327992).