SARS-CoV-2 Spike Glycoprotein (319-541 aa) Monoclonal antibody
SARS-CoV-2 Spike Glycoprotein (319-541 aa) Monoclonal Antibody for Neutralization, WB,ELISA
Host / Isotype
Mouse / IgG1
Cat no : 67758-1-Ig
|Positive WB detected in||Ag30688|
|Positive Neutralization detected in||Recombinant protein|
|Western Blot (WB)||WB : 1:5000-1:50000|
|NEUTRALIZATION||NEUTRALIZATION : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
67758-1-Ig targets SARS-CoV-2 Spike Glycoprotein (319-541 aa) in WB, Neutralization,ELISA applications and shows reactivity with Virus samples.
|Host / Isotype||Mouse / IgG1|
|Immunogen||SARS-CoV-2 Spike Glycoprotein (319-541 aa) fusion protein Ag30688|
|Full Name||SARS-CoV-2 Spike Protein|
|Calculated molecular weight||141 kDa|
|GenBank accession number||NC_045512|
|Gene symbol||COVID-19 S Protein|
|Gene ID (NCBI)||43740568|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).
Lyophilized format of this product is available.