COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal antibody

COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 27308-1-AP

Synonyms

COX 2, COX2, COX2/ Cyclooxygenase 2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2

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Validation Data Gallery

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  IF Staining of mouse lung using 27308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (27308-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  WB analysis of A549 using 27308-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse lung using 27308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (27308-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, mouse lung tissue
• Positive IHC detected in: human lung tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below

Product Information

The immunogen of 27308-1-AP is COX2/ Cyclooxygenase 2/ PTGS2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag26174
• Full Name: prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
• Calculated molecular weight: 604 aa, 68 kDa
• Observed molecular weight: 69 kDa
• GenBank accession number: BC013734
• Gene symbol: PTGS2
• Gene ID (NCBI): 5743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa(PMID:19656660 ). It also expresses a band of 39 kDa after unspecific cleavage(PMID:17509125 ). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains(PMID:14724276 ).

Publications

Species	Application	Title
mouse	WB	Molecules; Sweroside Alleviated LPS-Induced Inflammation via SIRT1 Mediating NF-κB and FOXO1 Signaling Pathways in RAW264.7 Cells.; Authors - Rui Wang
mouse	WB	Biosci Rep; Silencing of PTX3 alleviates LPS-induced inflammatory pain by regulating TLR4/NF-κB signaling pathway in mice.; Authors - Shuang Qi
human	WB	J Neuroimmunol; Protective effect of scorpion venom heat-resistant synthetic peptide against PM2.5-induced microglial polarization via TLR4-mediated autophagy activating PI3K/AKT/NF-κB signaling pathway.; Authors - Xin Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryan (Verified Customer) (02-27-2019)

Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown).

• Applications: Immunohistochemistry,Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish brain (Microglia) at 3dpf

COX2/ Cyclooxygenase 2/ PTGS2 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (Microglia) at 3dpf (Cat no:27308-1-AP)