COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal antibody

COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 27308-1-AP

Synonyms

COX 2, COX2, COX2/ Cyclooxygenase 2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2



Tested Applications

Positive WB detected inA549 cells, mouse lung tissue
Positive IHC detected inhuman lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27308-1-AP is COX2/ Cyclooxygenase 2/ PTGS2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag26174
Full Name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Calculated molecular weight 604 aa, 68 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC013734
Gene symbol PTGS2
Gene ID (NCBI) 5743
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa(PMID:19656660 ). It also expresses a band of 39 kDa after unspecific cleavage(PMID:17509125 ). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains(PMID:14724276 ).

Protocols

Product Specific Protocols
WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-APDownload protocol
IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-APDownload protocol
IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Molecules

Sweroside Alleviated LPS-Induced Inflammation via SIRT1 Mediating NF-κB and FOXO1 Signaling Pathways in RAW264.7 Cells.

Authors - Rui Wang
humanWB

Biochem Biophys Rep

TFAM knockdown-triggered mtDNA-nucleoid aggregation and a decrease in mtDNA copy number induce the reorganization of nucleoid populations and mitochondria-associated ER-membrane contacts

Authors - Koit Aasumets
humanWB

Aging (Albany NY)

Celecoxib reverses the glioblastoma chemo-resistance to temozolomide through mitochondrial metabolism.

Authors - Delong Yin
mouseWB,IF

ACS Appl Mater Interfaces

Programmable Polymeric Microneedles for Combined Chemotherapy and Antioxidative Treatment of Rheumatoid Arthritis.

Authors - Chaoxiong Wu
humanWB

Exp Ther Med

hsa-miR-15b-5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis.

Authors - Shujie Gan
mouseWB

FEBS Open Bio

Lipoxin A4 protects primary spinal cord neurons from Erastin-induced ferroptosis by activating the Akt/Nrf2/HO-1 signaling pathway.

Authors - Na Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ryan (Verified Customer) (02-27-2019)

Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown).

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish brain (Microglia) at 3dpf
COX2/ Cyclooxygenase 2/ PTGS2 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (Microglia) at 3dpf (Cat no:27308-1-AP)