- Featured Product
- KD/KO Validated
COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal antibody
COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 27308-1-AP
Validation Data Gallery
|Positive WB detected in
|A549 cells, mouse lung tissue
|Positive IHC detected in
|human lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|mouse lung tissue
|Western Blot (WB)
|WB : 1:500-1:1000
|IHC : 1:100-1:400
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
27308-1-AP targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat, pig
|Host / Isotype
|Rabbit / IgG
|COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag26174
|prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
|Calculated molecular weight
|604 aa, 68 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa(PMID:19656660 ). It also expresses a band of 39 kDa after unspecific cleavage(PMID:17509125 ). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains(PMID:14724276 ).
|Product Specific Protocols
|WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-AP
|IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-AP
|IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ryan (Verified Customer) (02-27-2019)
Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown).