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  • KD/KO Validated

COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal antibody

COX2/ Cyclooxygenase 2/ PTGS2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27308-1-AP

Synonyms

COX 2, COX2, COX2/ Cyclooxygenase 2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2



Tested Applications

Positive WB detected inA549 cells, mouse lung tissue
Positive IHC detected inhuman lung tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

27308-1-AP targets COX2/ Cyclooxygenase 2/ PTGS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag26174
Full Name prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
Calculated molecular weight 604 aa, 68 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC013734
Gene symbol PTGS2
Gene ID (NCBI) 5743
RRIDAB_2880838
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa(PMID:19656660 ). It also expresses a band of 39 kDa after unspecific cleavage(PMID:17509125 ). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains(PMID:14724276 ).

Protocols

Product Specific Protocols
WB protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-APDownload protocol
IHC protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-APDownload protocol
IF protocol for COX2/ Cyclooxygenase 2/ PTGS2 antibody 27308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Nucleic Acids Res

MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation

Authors - Bangming Jin
WB

Small

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

Authors - Jie Sun
mouseWB,IF

ACS Appl Mater Interfaces

Programmable Polymeric Microneedles for Combined Chemotherapy and Antioxidative Treatment of Rheumatoid Arthritis.

Authors - Chaoxiong Wu
mouseIHC

Redox Biol

Topical hypochlorous acid (HOCl) blocks inflammatory gene expression and tumorigenic progression in UV-exposed SKH-1 high risk mouse skin.

Authors - Jana Jandova
mouseWB,IHC

J Control Release

Combination of mitochondria impairment and inflammation blockade to combat metastasis

Authors - Xiaoli Yi
humanWB

Free Radic Biol Med

PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis

Authors - Qiong Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ryan (Verified Customer) (02-27-2019)

Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown).

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish brain (Microglia) at 3dpf
COX2/ Cyclooxygenase 2/ PTGS2 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (Microglia) at 3dpf (Cat no:27308-1-AP)