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  • KD/KO Validated

COX7A2L Polyclonal antibody

COX7A2L Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11416-1-AP

Synonyms

COX7a related protein, COX7A2L, COX7AR, COX7RP, EB1, SIG81



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11416-1-AP targets COX7A2L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX7A2L fusion protein Ag1980
Full Name cytochrome c oxidase subunit VIIa polypeptide 2 like
Calculated molecular weight 114 aa, 13 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC007095
Gene symbol COX7A2L
Gene ID (NCBI) 9167
RRIDAB_2245402
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c oxidase (COX) is the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. The mitochondrially encoded subunits function in electron transfer, and the nuclear encoded subunits may function in the regulation and assembly of the complex. COX7A2L (cytochrome c oxidase subunit 7A-related protein), also known as COX7AR or COX7RP, is an inner mitochondrial membrane protein. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. Recently it was found that DNA methylation of COX7A2L had a significant association with therapy response in patients with breast cancer. In Alzheimer's disease the expression of COX7A2L was reduced.

Protocols

Product Specific Protocols
WB protocol for COX7A2L antibody 11416-1-APDownload protocol
IHC protocol for COX7A2L antibody 11416-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Science

Supercomplex assembly determines electron flux in the mitochondrial electron transport chain.

Authors - Esther Lapuente-Brun
humanWB

EMBO J

Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes.

Authors - Teresa Lobo-Jarne
humanWB

Cell Rep

Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics.

Authors - Teresa Lobo-Jarne
  • KO Validated

Free Radic Biol Med

Cardioprotective strategies preserve the stability of respiratory chain supercomplexes and reduce oxidative stress in reperfused ischemic hearts.

Authors - I Ramírez-Camacho
humanWB

Cell Rep

COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation.

Authors - Rafael Pérez-Pérez
humanWB,IHC,IF

J Pathol

Clinical response to chemotherapy in oesophageal adenocarcinoma patients is linked to defects in mitochondria.

Authors - Aichler Michaela M
  • KD Validated