COX7A2L Polyclonal antibody

• Featured Product
• KD/KO Validated

COX7A2L Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 11416-1-AP

Synonyms

COX7a related protein, COX7A2L, COX7AR, COX7RP, EB1, SIG81

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Validation Data Gallery

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  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 40x lens).

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  WB analysis of mouse brain using 11416-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11416-1-AP (COX7A2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue, rat liver tissue
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

11416-1-AP targets COX7A2L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COX7A2L fusion protein Ag1980
• Full Name: cytochrome c oxidase subunit VIIa polypeptide 2 like
• Calculated molecular weight: 114 aa, 13 kDa
• Observed molecular weight: 14 kDa
• GenBank accession number: BC007095
• Gene symbol: COX7A2L
• Gene ID (NCBI): 9167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cytochrome c oxidase (COX) is the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. The mitochondrially encoded subunits function in electron transfer, and the nuclear encoded subunits may function in the regulation and assembly of the complex. COX7A2L (cytochrome c oxidase subunit 7A-related protein), also known as COX7AR or COX7RP, is an inner mitochondrial membrane protein. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. Recently it was found that DNA methylation of COX7A2L had a significant association with therapy response in patients with breast cancer. In Alzheimer's disease the expression of COX7A2L was reduced.

Publications

Species	Application	Title
		Free Radic Biol Med; Cardioprotective strategies preserve the stability of respiratory chain supercomplexes and reduce oxidative stress in reperfused ischemic hearts.; Authors - I Ramírez-Camacho
human	WB	Cell Rep; Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis.; Authors - Alba Timón-Gómez ; KO Validated
mouse	WB	Cell Rep; Respiratory Supercomplexes Promote Mitochondrial Efficiency and Growth in Severely Hypoxic Pancreatic Cancer.; Authors - Kate E R Hollinshead
human	IHC	J Proteomics; MALDI imaging mass spectrometry reveals COX7A2, TAGLN2 and S100-A10 as novel prognostic markers in Barrett's adenocarcinoma.; Authors - Mareike Elsner
mouse	WB	Sci Rep; Deficiency of COX7RP, a mitochondrial supercomplex assembly promoting factor, lowers blood glucose level in mice.; Authors - Sachiko Shiba ; KD Validated
human,mouse	WB,IF	Sci Rep; COX7AR is a Stress-inducible Mitochondrial COX Subunit that Promotes Breast Cancer Malignancy.; Authors - Kezhong Zhang