COX7A2L Polyclonal antibody

• Featured Product
• KD/KO Validated

COX7A2L Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 11416-1-AP

Synonyms

COX7a related protein, COX7A2L, COX7AR, COX7RP, EB1, SIG81

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Validation Data Gallery

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  WB analysis using 11416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11416-1-AP (COX7A2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 11416-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11416-1-AP (COX7A2L antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

11416-1-AP targets COX7A2L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COX7A2L fusion protein Ag1980
• Full Name: cytochrome c oxidase subunit VIIa polypeptide 2 like
• Calculated molecular weight: 114 aa, 13 kDa
• Observed molecular weight: 14 kDa
• GenBank accession number: BC007095
• Gene symbol: COX7A2L
• Gene ID (NCBI): 9167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome c oxidase (COX) is the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. The mitochondrially encoded subunits function in electron transfer, and the nuclear encoded subunits may function in the regulation and assembly of the complex. COX7A2L (cytochrome c oxidase subunit 7A-related protein), also known as COX7AR or COX7RP, is an inner mitochondrial membrane protein. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. Recently it was found that DNA methylation of COX7A2L had a significant association with therapy response in patients with breast cancer. In Alzheimer's disease the expression of COX7A2L was reduced.

Publications

Species	Application	Title
mouse	WB	Science; Supercomplex assembly determines electron flux in the mitochondrial electron transport chain.; Authors - Esther Lapuente-Brun
human	WB	EMBO J; Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes.; Authors - Teresa Lobo-Jarne
human	WB	Cell Rep; Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics.; Authors - Teresa Lobo-Jarne ; KO Validated
human	WB	Cell Rep; COX7A2L Is a Mitochondrial Complex III Binding Protein that Stabilizes the III2+IV Supercomplex without Affecting Respirasome Formation.; Authors - Rafael Pérez-Pérez
human	WB,IHC,IF	J Pathol; Clinical response to chemotherapy in oesophageal adenocarcinoma patients is linked to defects in mitochondria.; Authors - Aichler Michaela M ; KD Validated
mouse	WB	Neurobiol Aging; Up-regulation of key microRNAs, and inverse down-regulation of their predicted oxidative phosphorylation target genes, during aging in mouse brain.; Authors - Li Na N