CPLX1 Polyclonal antibody

CPLX1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10246-2-AP

Synonyms

complexin 1, Complexin I, complexin1, CPLX1, CPX I, CPX1, Synaphin 2



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10246-2-AP targets CPLX1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, macaque, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPLX1 fusion protein Ag0387
Full Name complexin 1
Calculated molecular weight 15 kDa
Observed molecular weight 15-20 kDa
GenBank accession numberBC002471
Gene symbol CPLX1
Gene ID (NCBI) 10815
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Complexin 1 (CPLX1) is a member of the complexin/synaphin gene family, which are cytosolic proteins that function in synaptic vesicle exocytosis. Complexins are enriched in neurons where they colocalize with syntaxin and SNAP-25. Complexins bind weakly to syntaxin alone and not at all to synaptobrevin and SNAP-25, but strongly to the SNAP receptor-core complex composed of these three molecules. Compexins also compete with alpha-SNAP for binding to the core complex but not with other interacting molecules, including synaptotagmin I, and regulate the sequential interactions of alpha-SNAP and synaptotagmins with the SNAP receptor during exocytosis. CLPX1 binds to the SNAP receptor complex and disrupts it, leading to the release of transmitters. Alterations of complexins may contribute to the molecular substrate for abnormalities of neural connectivity in severe mental disorders.

Protocols

Product Specific Protocols
WB protocol for CPLX1 antibody 10246-2-APDownload protocol
IHC protocol for CPLX1 antibody 10246-2-APDownload protocol
IF protocol for CPLX1 antibody 10246-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Dev Neurosci

Molecular evidence that cortical synaptic growth predominates during the first decade of life in humans.

Authors - Webster Maree J MJ
macaqueIHC

J Virol

Acute Simian Varicella Virus Infection Causes Robust and Sustained Changes in Gene Expression in the Sensory Ganglia.

Authors - Nicole Arnold
mouseWB

Free Radic Biol Med

NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke.

Authors - Wang Ziying Z
mouseWB

J Neurosci

Loss of Protein Arginine Methyltransferase 8 Alters Synapse Composition and Function, Resulting in Behavioral Defects.

Authors - Jay Penney
mouseWB

Nat Neurosci

The schizophrenia risk gene product miR-137 alters presynaptic plasticity.

Authors - Sandra Siegert