Tested Applications
| Positive WB detected in | mouse liver tissue, HeLa cells, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18703-1-AP targets CPS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | carbamoyl-phosphate synthetase 1, mitochondrial |
| Calculated Molecular Weight | 165 kDa |
| Observed Molecular Weight | 140-165 kDa |
| GenBank Accession Number | BC140943 |
| Gene Symbol | CPS1 |
| Gene ID (NCBI) | 1373 |
| RRID | AB_2084238 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31327 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CPS1 is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. Expressed primarily in the liver and small intestine, CPS1 can be used as a marker for mitochondria in these tissues. In addition, CPS1 expression appears to be lost in adenocarcinomas of the small intestine. The antibody can recognize two isoforms of this gene around 165 kDa and 116 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CPS1 antibody 18703-1-AP | Download protocol |
| IHC protocol for CPS1 antibody 18703-1-AP | Download protocol |
| WB protocol for CPS1 antibody 18703-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
18703-1-AP is a CPS1 (Carbamoyl-Phosphate Synthase 1) antibody with 21 total citations. It has been published in journals including Nature, Cancer Cell, Nature Communications, Theranostics, Advanced Science, EMBO Molecular Medicine, Molecular Metabolism, iScience, and Frontiers in Immunology. Research fields cover ferroptosis, hepatocellular carcinoma, lung cancer metastasis, urea cycle disorders, non-alcoholic fatty liver disease, mitochondrial myopathy, glioma, bladder cancer, and hepatic lipid metabolism. Applications are predominantly Western blot, with some IF and IHC.
| Species | Application | Title |
|---|---|---|
Cancer Cell Inhibition of De Novo NAD(+) Synthesis by Oncogenic URI Causes Liver Tumorigenesis through DNA Damage. | ||
Nat Commun Plasmodium sporozoite phospholipid scramblase interacts with mammalian carbamoyl-phosphate synthetase 1 to infect hepatocytes. | ||
Theranostics Targeting CPS1 attenuates lung cancer metastasis by regulating EMT through an epigenetic mechanism.
| ||
Adv Sci (Weinh) Cardiac Urea Cycle Activation by Time-Restricted Feeding Protects Against Pressure Overload-Induced Heart Failure | ||
EMBO Mol Med A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy |
Reviews
What customers say
One reviewer rated this antibody 4 out of 5 stars, noting it performed well for Western blot at a 1:1000 dilution on liver tissue. Overall, the feedback is positive, with the user confirming reliable WB performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (11-13-2025) | good for Western blot
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