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  • KD/KO Validated

CPSF6 Polyclonal antibody

CPSF6 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 15489-1-AP


CFIM, CFIM68, CPSF 68 kDa subunit, CPSF6, HPBRII 4, HPBRII 7, Protein HPBRII 4/7

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, PC-3 cells, MDA-MB-231 cells, SW480 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSW480 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15489-1-AP targets CPSF6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPSF6 fusion protein Ag7852
Full Name cleavage and polyadenylation specific factor 6, 68kDa
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 55-68 kDa
GenBank Accession NumberBC000714
Gene Symbol CPSF6
Gene ID (NCBI) 11052
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The binding of Cleavage factor Im (CFIM), also known as CPSF6, to the pre-mRNA is one of the earliest steps in the assembly of the cleavage and polyadenylation machinery and facilitates the recruitment of other processing factors. CFIM is required for the first step in pre-mRNA 3′-end processing and can be reconstituted in vitro from its heterologously expressed 25- and 68-kDa subunits. It involved in RNA binding, protein-protein interactions, and subcellular localization [PMID:15169763]. In addition, it is a pre-mRNA processing protein that dynamically shuttles between the nucleus and the cytoplasm and contains a C-terminal nuclear-targeting arginine/serine-rich (RS-) domain of the type bound by TNPO3[PMID:15169763,19864460].


Product Specific Protocols
WB protocol for CPSF6 antibody 15489-1-APDownload protocol
IHC protocol for CPSF6 antibody 15489-1-APDownload protocol
IF protocol for CPSF6 antibody 15489-1-APDownload protocol
IP protocol for CPSF6 antibody 15489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

A Quantitative Genetic Interaction Map of HIV Infection.

Authors - David E Gordon

PLoS Pathog

In Vivo Functions of CPSF6 for HIV-1 as Revealed by HIV-1 Capsid Evolution in HLA-B27-Positive Subjects.

Authors - Matthew S Henning


Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

Authors - Melissa Kane

J Virol

Cell type-dependent escape of capsid inhibitors by simian immunodeficiency virus SIVcpz.

Authors - Augustin Penda Twizerimana


Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Authors - Mio Iwasaki

J Virol

Roles of capsid-interacting host factors in multimodal inhibition of HIV-1 by PF74.

Authors - Akatsuki Saito
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tatyana (Verified Customer) (03-11-2023)

The signal is not very bright but specific (siRNA knockdown verified). Predominantly nuclear, as expected. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; overnight incubation (no blocking step).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human, SH-SY5Y cells