Validation Data Gallery
|Positive WB detected in||HeLa cells, Jurkat cells, PC-3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human kidney tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
15489-1-AP targets CPSF6 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CPSF6 fusion protein Ag7852|
|Full Name||cleavage and polyadenylation specific factor 6, 68kDa|
|Calculated molecular weight||59 kDa|
|Observed molecular weight||55-68 kDa|
|GenBank accession number||BC000714|
|Gene ID (NCBI)||11052|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The binding of Cleavage factor Im (CFIM), also known as CPSF6, to the pre-mRNA is one of the earliest steps in the assembly of the cleavage and polyadenylation machinery and facilitates the recruitment of other processing factors. CFIM is required for the first step in pre-mRNA 3′-end processing and can be reconstituted in vitro from its heterologously expressed 25- and 68-kDa subunits. It involved in RNA binding, protein-protein interactions, and subcellular localization [PMID:15169763]. In addition, it is a pre-mRNA processing protein that dynamically shuttles between the nucleus and the cytoplasm and contains a C-terminal nuclear-targeting arginine/serine-rich (RS-) domain of the type bound by TNPO3[PMID:15169763,19864460].
A Quantitative Genetic Interaction Map of HIV Infection.
In Vivo Functions of CPSF6 for HIV-1 as Revealed by HIV-1 Capsid Evolution in HLA-B27-Positive Subjects.
Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.
Cell type-dependent escape of capsid inhibitors by simian immunodeficiency virus SIVcpz.
Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.
Roles of capsid-interacting host factors in multimodal inhibition of HIV-1 by PF74.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tatyana (Verified Customer) (03-11-2023)
The signal is not very bright but specific (siRNA knockdown verified). Predominantly nuclear, as expected. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; overnight incubation (no blocking step).