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CPT1B-specific Polyclonal antibody
CPT1B-specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 22170-1-AP
|Positive WB detected in||mouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue|
|Positive IP detected in||mouse heart tissue, mouse skeletal muscle tissue|
|Positive IHC detected in||mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22170-1-AP is CPT1B-specific Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CPT1B-specific fusion protein Ag17840|
|Full Name||carnitine palmitoyltransferase 1B (muscle)|
|Calculated molecular weight||567 aa, 64 kDa|
|Observed molecular weight||75-85 kDa|
|GenBank accession number||BC131570|
|Gene ID (NCBI)||1375|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Carnitine palmitoyltransferases (CPTs) mediate the transport of long chain fatty acyl groups into the mitochondrial matrix to undergo b-oxidation. Type I CPT resides in the outer mitochondrial membrane. Mammalian tissues express three isoforms: CPT1A (liver), CPT1B (muscle and heart), and CPT1C (brain). Several isoforms of CPT1B exist due to the alternative splicing, with predicted MW of 88/84/79/66 kDa.
|Product Specific Protocols|
|WB protocol for CPT1B-specific antibody 22170-1-AP||Download protocol|
|IHC protocol for CPT1B-specific antibody 22170-1-AP||Download protocol|
|IF protocol for CPT1B-specific antibody 22170-1-AP||Download protocol|
|IP protocol for CPT1B-specific antibody 22170-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mol Cell Endocrinol
Novel metabolic disorders in skeletal muscle of Lipodystrophic Bscl2/Seipin deficient mice.
Modulation of Fatty Acid-Related Genes in the Response of H9c2 Cardiac Cells to Palmitate and n-3 Polyunsaturated Fatty Acids.
Targeting CPT1B as a potential therapeutic strategy in castration-resistant and enzalutamide-resistant prostate cancer.
Am J Physiol Heart Circ Physiol
TXNIP regulates myocardial fatty acid oxidation via miR-33a signaling.
Cell Tissue Res
Cardiac-specific LRP6 knockout induces lipid accumulation through Drp1/CPT1b pathway in adult mice.
Am J Physiol Endocrinol Metab
Combined effects of a ketogenic diet and exercise training alter mitochondrial and peroxisomal substrate oxidative capacity in skeletal muscle.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
James (Verified Customer) (04-01-2021)
Green channel is CPT1B.