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  • KD/KO Validated

CPT1B-specific Polyclonal antibody

CPT1B-specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22170-1-AP

Synonyms

CPT I, CPT1 M, CPT1B, CPTI M, KIAA1670, M CPT1, MCCPT1



Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22170-1-AP is CPT1B-specific Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPT1B-specific fusion protein Ag17840
Full Name carnitine palmitoyltransferase 1B (muscle)
Calculated molecular weight 567 aa, 64 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberBC131570
Gene symbol CPT1B
Gene ID (NCBI) 1375
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Carnitine palmitoyltransferases (CPTs) mediate the transport of long chain fatty acyl groups into the mitochondrial matrix to undergo b-oxidation. Type I CPT resides in the outer mitochondrial membrane. Mammalian tissues express three isoforms: CPT1A (liver), CPT1B (muscle and heart), and CPT1C (brain). Several isoforms of CPT1B exist due to the alternative splicing, with predicted MW of 88/84/79/66 kDa.

Protocols

Product Specific Protocols
WB protocol for CPT1B-specific antibody 22170-1-APDownload protocol
IHC protocol for CPT1B-specific antibody 22170-1-APDownload protocol
IF protocol for CPT1B-specific antibody 22170-1-APDownload protocol
IP protocol for CPT1B-specific antibody 22170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell Endocrinol

Novel metabolic disorders in skeletal muscle of Lipodystrophic Bscl2/Seipin deficient mice.

Authors - Wenqiong Xu
ratWB

Cells

Modulation of Fatty Acid-Related Genes in the Response of H9c2 Cardiac Cells to Palmitate and n-3 Polyunsaturated Fatty Acids.

Authors - Silvia Cetrullo
mouseWB

Nanomicro Lett

Nanoparticle-Mediated Lipid Metabolic Reprogramming of T Cells in Tumor Microenvironments for Immunometabolic Therapy.

Authors - Dongyoon Kim
humanWB

J Sport Health Sci

Exercise promotes angiogenesis by enhancing endothelial cell fatty acid use via liver-derived extracellular vesicle miR-122-5p.

Authors - Jing Lou
mouseWB

Appl Physiol Nutr Metab

Exercise improves lipid metabolism disorders induced by high-fat diet in a SESN2/JNK- independent manner.

Authors - Tianyi Wang
humanWB

Prostate

Targeting CPT1B as a potential therapeutic strategy in castration-resistant and enzalutamide-resistant prostate cancer.

Authors - Mierxiati Abudurexiti
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

James (Verified Customer) (04-01-2021)

Green channel is CPT1B.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: lung
CPT1B-specific Antibody Immunofluorescence validation (1:100 dilution) in lung (Cat no:22170-1-AP)