CPT1B-specific Polyclonal antibody

• Featured Product
• KD/KO Validated

CPT1B-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

31

Reviews (1)

Cat no : 22170-1-AP

Synonyms

CPT I, CPT1 M, CPT1B, CPT1B-specific, CPTI M, KIAA1670, M CPT1, MCCPT1

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Validation Data Gallery

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  WB analysis using 22170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 22170-1-AP

  Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 22170-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22170-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22170-1-AP (CPT1B-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 22170-1-AP

  IP result of anti-CPT1B-specific (IP:22170-1-AP, 4ug; Detection:22170-1-AP 1:600) with mouse heart tissue lysate 3600 ug.

• 

  IP experiment of mouse skeletal muscle using 22170-1-AP

  IP Result of anti-CPT1B-specific (IP:22170-1-AP, 4ug; Detection:22170-1-AP 1:600) with mouse skeletal muscle tissue lysate 2200ug.

• 

  IHC staining of mouse heart using 22170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22170-1-AP (CPT1B-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22170-1-AP (CPT1B-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22170-1-AP (CPT1B-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 22170-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 22170-1-AP (CPT1B-specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 22170-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 22170-1-AP (CPT1B-specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse heart tissue, mouse skeletal muscle tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 28 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 22170-1-AP is CPT1B-specific Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, ewes, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CPT1B-specific fusion protein Ag17840
• Full Name: carnitine palmitoyltransferase 1B (muscle)
• Calculated molecular weight: 567 aa, 64 kDa
• Observed molecular weight: 75-85 kDa
• GenBank accession number: BC131570
• Gene symbol: CPT1B
• Gene ID (NCBI): 1375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Carnitine palmitoyltransferases (CPTs) mediate the transport of long chain fatty acyl groups into the mitochondrial matrix to undergo b-oxidation. Type I CPT resides in the outer mitochondrial membrane. Mammalian tissues express three isoforms: CPT1A (liver), CPT1B (muscle and heart), and CPT1C (brain). Several isoforms of CPT1B exist due to the alternative splicing, with predicted MW of 88/84/79/66 kDa.

Publications

Species	Application	Title
mouse	WB	Mol Cell Endocrinol; Novel metabolic disorders in skeletal muscle of Lipodystrophic Bscl2/Seipin deficient mice.; Authors - Wenqiong Xu
rat	WB	Cells; Modulation of Fatty Acid-Related Genes in the Response of H9c2 Cardiac Cells to Palmitate and n-3 Polyunsaturated Fatty Acids.; Authors - Silvia Cetrullo
mouse	WB	Nanomicro Lett; Nanoparticle-Mediated Lipid Metabolic Reprogramming of T Cells in Tumor Microenvironments for Immunometabolic Therapy.; Authors - Dongyoon Kim
human	WB	J Sport Health Sci; Exercise promotes angiogenesis by enhancing endothelial cell fatty acid use via liver-derived extracellular vesicle miR-122-5p.; Authors - Jing Lou
mouse	WB,IP	Cell Rep; PHDs/CPT1B/VDAC1 axis regulates long-chain fatty acid oxidation in cardiomyocytes.; Authors - Aude Angelini
mouse	WB	Int J Mol Sci; Differential Effects of 17,18-EEQ and 19,20-EDP Combined with Soluble Epoxide Hydrolase Inhibitor t-TUCB on Diet-Induced Obesity in Mice.; Authors - Yang Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (04-01-2021)

Green channel is CPT1B.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: lung

CPT1B-specific Antibody Immunofluorescence validation (1:100 dilution) in lung (Cat no:22170-1-AP)