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CPT1B-specific Polyclonal antibody
CPT1B-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 22170-1-AP
Validation Data Gallery
|Positive WB detected in||mouse heart tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat heart tissue|
|Positive IP detected in||mouse heart tissue, mouse skeletal muscle tissue|
|Positive IHC detected in||mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
22170-1-AP targets CPT1B-specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, zebrafish, ewes|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CPT1B-specific fusion protein Ag17840|
|Full Name||carnitine palmitoyltransferase 1B (muscle)|
|Calculated molecular weight||567 aa, 64 kDa|
|Observed molecular weight||75-85 kDa|
|GenBank accession number||BC131570|
|Gene ID (NCBI)||1375|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Carnitine palmitoyltransferases (CPTs) mediate the transport of long chain fatty acyl groups into the mitochondrial matrix to undergo b-oxidation. Type I CPT resides in the outer mitochondrial membrane. Mammalian tissues express three isoforms: CPT1A (liver), CPT1B (muscle and heart), and CPT1C (brain). Several isoforms of CPT1B exist due to the alternative splicing, with predicted MW of 88/84/79/66 kDa.
|Product Specific Protocols|
|WB protocol for CPT1B-specific antibody 22170-1-AP||Download protocol|
|IHC protocol for CPT1B-specific antibody 22170-1-AP||Download protocol|
|IF protocol for CPT1B-specific antibody 22170-1-AP||Download protocol|
|IP protocol for CPT1B-specific antibody 22170-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
James (Verified Customer) (04-01-2021)
Green channel is CPT1B.