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  • KD/KO Validated

CPT1B-specific Polyclonal antibody

CPT1B-specific Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (4)





Cat no : 22170-1-AP


CPT I, CPT1 M, CPT1B, CPT1B-specific, CPTI M, KIAA1670, M CPT1, MCCPT1

Tested Applications

Positive WB detected inmouse heart tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22170-1-AP targets CPT1B-specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, sheep, zebrafish, pig, ewes
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPT1B-specific fusion protein Ag17840
Full Name carnitine palmitoyltransferase 1B (muscle)
Calculated Molecular Weight 567 aa, 64 kDa
Observed Molecular Weight 75-85 kDa
GenBank Accession NumberBC131570
Gene Symbol CPT1B
Gene ID (NCBI) 1375
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carnitine palmitoyltransferases (CPTs) mediate the transport of long chain fatty acyl groups into the mitochondrial matrix to undergo b-oxidation. Type I CPT resides in the outer mitochondrial membrane. Mammalian tissues express three isoforms: CPT1A (liver), CPT1B (muscle and heart), and CPT1C (brain). Several isoforms of CPT1B exist due to the alternative splicing, with predicted MW of 88/84/79/66 kDa.


Product Specific Protocols
WB protocol for CPT1B-specific antibody 22170-1-APDownload protocol
IHC protocol for CPT1B-specific antibody 22170-1-APDownload protocol
IF protocol for CPT1B-specific antibody 22170-1-APDownload protocol
IP protocol for CPT1B-specific antibody 22170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Metab

A mechanosensitive lipolytic factor in the bone marrow promotes osteogenesis and lymphopoiesis.

Authors - Hui Peng

Cell Metab

Global Analysis of Plasma Lipids Identifies Liver-Derived Acylcarnitines as a Fuel Source for Brown Fat Thermogenesis.

Authors - Judith Simcox
  • KD Validated

Adv Sci (Weinh)

m6A-Modified circTET2 Interacting with HNRNPC Regulates Fatty Acid Oxidation to Promote the Proliferation of Chronic Lymphocytic Leukemia

Authors - Zijuan Wu

Nat Commun

Mechanism of action and therapeutic route for a muscular dystrophy caused by a genetic defect in lipid metabolism.

Authors - Mahtab Tavasoli


LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells.

Authors - Myeong-Hwa Song

Cardiovasc Res

Myocardial brain-derived neurotrophic factor regulates cardiac bioenergetics through the transcription factor Yin Yang 1.

Authors - Xue Yang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Xhuljana (Verified Customer) (03-01-2024)

Tried in WB in C2C12 lysate, 30 *g

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C2C12 cells
CPT1B-specific Antibody Western Blot validation (1:1000 dilution) in C2C12 cells (Cat no:22170-1-AP)

James (Verified Customer) (04-01-2021)

Green channel is CPT1B.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: lung
CPT1B-specific Antibody Immunofluorescence validation (1:100 dilution) in lung (Cat no:22170-1-AP)