CRBN Polyclonal antibody, PBS Only

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  WB analysis using 28494-1-AP (same clone as 28494-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28494-1-AP (same clone as 28494-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28494-1-AP (same clone as 28494-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 28494-1-AP (same clone as 28494-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 28494-1-AP (CRBN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 28494-1-AP (same clone as 28494-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 28494-1-AP (same clone as 28494-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 28494-1-AP (same clone as 28494-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 28494-1-AP (same clone as 28494-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28494-1-AP (CRBN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

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  IF Staining of COLO 320 using 28494-1-AP (same clone as 28494-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using CRBN antibody (28494-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28494-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CRBN Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 28494-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Protein cereblon, cereblon, AD-006

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Product Information

28494-1-PBS targets CRBN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRBN fusion protein Ag29353
• Full Name: cereblon
• Calculated Molecular Weight: 442 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC017419
• Gene Symbol: CRBN
• Gene ID (NCBI): 51185
• RRID: AB_2881155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96SW2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).