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Gamma Cystathionase Monoclonal antibody
Gamma Cystathionase Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
human, mouse, rat and More (1)
WB, IHC, IF, ELISA
Cat no : 60234-1-Ig
Validation Data Gallery
|Positive WB detected in||LNCaP cells, HSC-T6 cells, PC-3 cells, HEK-293 cells, HeLa cells, A431 cells, NIH/3T3 cells, HepG2 cells, K-562 cells|
|Positive IHC detected in||human lung cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|WB||See 16 publications below|
|IHC||See 2 publications below|
|IF||See 3 publications below|
The immunogen of 60234-1-Ig is Gamma Cystathionase Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, canine|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Gamma Cystathionase fusion protein Ag2872|
|Full Name||cystathionase (cystathionine gamma-lyase)|
|Calculated molecular weight||405 aa, 45 kDa|
|Observed molecular weight||40-45 kDa|
|GenBank accession number||BC015807|
|Gene ID (NCBI)||1491|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CTH, also named as Gamma-cystathionase and CSE, belongs to the transsulfuration enzymes family. It catalyzes the last step in the transsulfuration pathway from methionine to cysteine. CTH converts two cysteine molecules to lanthionine and hydrogen sulfide. CTH can also accept homocysteine as substrate. It specificity depends on the levels of the endogenous substrates. CTH is the major H2S-producing enzyme in kidney, liver, vascular smooth muscle cells and enterocytes. The endogenous production of H2S plays a significant role in the regulation of cellular functions, including cell growth, hyperpolarization of cell membranes, modulation of neuronal excitability and relaxation of smooth muscle cells. The CSE/H2S pathway is upregulated in the heart in a murine model of CVB3-induced myocarditis and that inhibition of endogenous H2S is beneficial to treatment early in the disease while administration of exogenous H2S is protective to infected myocardium during the later stage. Mutations in the gene encoding CTH can result in the autosomal recessive disease cystathioninuria; a disorder characterized by the unusual accumulation of plasma cystathionine causing increased urinary excretion. Both male and female CTH-null mice showed hypercystathioninemia and hyperhomocysteinemia, but not hypermethioninemia. CSE has also been reported to be expressed in endothelial cells and contribute to endothelium-dependent vasorelaxation. It can be detected a minor 36 kDa band probably representing a degradative intermediate except the major 43 kDa band in vitamin B6-deficient rat liver(PMID:8660672). CTH also can be detected as ~70kD in rat liver (PMID: 18974309).
|Product Specific Protocols|
|WB protocol for Gamma Cystathionase antibody 60234-1-Ig||Download protocol|
|IHC protocol for Gamma Cystathionase antibody 60234-1-Ig||Download protocol|
|IF protocol for Gamma Cystathionase antibody 60234-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Dexamethasone sensitizes to ferroptosis by glucocorticoid receptor-induced dipeptidase-1 expression and glutathione depletion.
Vascular smooth muscle cell-derived hydrogen sulfide promotes atherosclerotic plaque stability via TFEB (transcription factor EB)-mediated autophagy.
The novel H2S-donor 4-carboxyphenyl isothiocyanate promotes cardioprotective effects against ischemia/reperfusion injury through activation of mitoKATP channels and reduction of oxidative stress.
Endogenous hydrogen sulfide regulates xCT stability through persulfidation of OTUB1 at cysteine 91 in colon cancer cells.
Vascular Effects of Low-Dose ACE2 Inhibitor MLN-4760-Benefit or Detriment in Essential Hypertension?
Hydrogen sulfide ameliorated preeclampsia via suppression of toll-like receptor 4-activated inflammation in the rostral ventrolateral medulla of rats.