Tested Applications
| Positive WB detected in | A431 cells, PC-3 cells, K-562 cells, NIH/3T3 cells, Raji cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10992-1-AP targets CSNK2A1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1452 Product name: Recombinant human CSNK2A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-391 aa of BC011668 Sequence: MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ Predict reactive species |
| Full Name | casein kinase 2, alpha 1 polypeptide |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC011668 |
| Gene Symbol | CSNK2A1 |
| Gene ID (NCBI) | 1457 |
| RRID | AB_2245531 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P68400 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CSNK2A1 antibody 10992-1-AP | Download protocol |
| IHC protocol for CSNK2A1 antibody 10992-1-AP | Download protocol |
| IP protocol for CSNK2A1 antibody 10992-1-AP | Download protocol |
| WB protocol for CSNK2A1 antibody 10992-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CSNK2A1 (CK2α) Polyclonal antibody (Cat# 10992-1-AP) has 47 citations. It appears in high-impact journals including Nature Immunology, Nature Communications, Cell Death & Differentiation, Nucleic Acids Research, Advanced Science, Oncogene, Cell Reports, and EMBO Reports. Research fields span oncology (colorectal, pancreatic, ovarian, esophageal, thyroid cancers), neurology (Parkinson's, stroke, synucleinopathy), immunology, cardiovascular disease, aging, and fundamental cell biology such as DNA replication, transcription, and signaling pathways. Applications are predominantly Western blot, with IP, IHC, IF, CoIP, and ELISA.
| Species | Application | Title |
|---|---|---|
Nat Immunol GIMAP5 deficiency reveals a mammalian ceramide-driven longevity assurance pathway | ||
Nat Commun CK2-induced cooperation of HHEX with the YAP-TEAD4 complex promotes colorectal tumorigenesis | ||
Exp Hematol Oncol Inhibition of protein kinase CK2 remodels the tumor immune microenvironment and sensitizes lung cancer to PD-L1 blockade. | ||
Redox Biol Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury. | ||
Nucleic Acids Res CK2 kinase-mediated PHF8 phosphorylation controls TopBP1 stability to regulate DNA replication.
| ||
Nucleic Acids Res ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters. |
Reviews
What customers say
The single review for this product, used in Western Blot on prostate/RWPE-1 cells at a 1:5000 dilution, gave it a 5-star rating. The reviewer noted some background bands appear at high contrast, but overall the signal is clean and appropriate for interpretation at a standard contrast level.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Janeen (Verified Customer) (07-22-2021) | There are some background bands at high contrast. But overall, a nice clean signal at a contrast level appropriate for interpretation.
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