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  • KD/KO Validated

CSNK2A1 Polyclonal antibody

CSNK2A1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10992-1-AP

Synonyms

Casein kinase II subunit alpha, CK II alpha, CK2a, CK2A1, CK2α, CKII, CSNK2A1



Tested Applications

Positive WB detected inPC-3 cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, Raji cells, A431 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10992-1-AP targets CSNK2A1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSNK2A1 fusion protein Ag1452
Full Name casein kinase 2, alpha 1 polypeptide
Calculated molecular weight 45 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC011668
Gene symbol CSNK2A1
Gene ID (NCBI) 1457
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.

Protocols

Product Specific Protocols
WB protocol for CSNK2A1 antibody 10992-1-APDownload protocol
IHC protocol for CSNK2A1 antibody 10992-1-APDownload protocol
IF protocol for CSNK2A1 antibody 10992-1-APDownload protocol
IP protocol for CSNK2A1 antibody 10992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Protein kinase CK2α catalytic subunit is overexpressed and serves as an unfavorable prognostic marker in primary hepatocellular carcinoma.

Authors - Hong-Xia Zhang
humanWB

J Burn Care Res

Long-wave Ultraviolet Ray Promotes Inflammation in Keloid-derived Fibroblasts by Activating P38-NFκB1 Signaling Pathway.

Authors - Xingtang Niu
humanWB

Biochem Cell Biol

Lentivirus-mediated down-regulation of CK2α inhibits proliferation and induces apoptosis of malignant lymphoma and leukemia cells.

Authors - Li Jiang
  • KD Validated
humanWB

FEBS Open Bio

CSNK2A1-mediated phosphorylation of HMGA2 modulates cisplatin resistance in cervical cancer.

Authors - Zhan Shi
mouseWB

Biochem Biophys Res Commun

β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells.

Authors - Yan Zhang
humanWB,IHC

PLoS One

Increased expression of protein kinase CK2α correlates with poor patient prognosis in epithelial ovarian cancer.

Authors - Zebiao Ma
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Janeen (Verified Customer) (07-22-2021)

There are some background bands at high contrast. But overall, a nice clean signal at a contrast level appropriate for interpretation.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: prostate/ RWPE-1
CSNK2A1 Antibody Western Blot validation (1:5000 dilution) in prostate/ RWPE-1 (Cat no:10992-1-AP)