Validation Data Gallery
|Positive WB detected in||PC-3 cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, Raji cells, A431 cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10992-1-AP targets CSNK2A1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CSNK2A1 fusion protein Ag1452|
|Full Name||casein kinase 2, alpha 1 polypeptide|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||40-45 kDa|
|GenBank accession number||BC011668|
|Gene ID (NCBI)||1457|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.
|Product Specific Protocols|
|WB protocol for CSNK2A1 antibody 10992-1-AP||Download protocol|
|IHC protocol for CSNK2A1 antibody 10992-1-AP||Download protocol|
|IF protocol for CSNK2A1 antibody 10992-1-AP||Download protocol|
|IP protocol for CSNK2A1 antibody 10992-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Protein kinase CK2α catalytic subunit is overexpressed and serves as an unfavorable prognostic marker in primary hepatocellular carcinoma.
J Burn Care Res
Long-wave Ultraviolet Ray Promotes Inflammation in Keloid-derived Fibroblasts by Activating P38-NFκB1 Signaling Pathway.
Biochem Cell Biol
Lentivirus-mediated down-regulation of CK2α inhibits proliferation and induces apoptosis of malignant lymphoma and leukemia cells.
FEBS Open Bio
CSNK2A1-mediated phosphorylation of HMGA2 modulates cisplatin resistance in cervical cancer.
Biochem Biophys Res Commun
β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells.
Increased expression of protein kinase CK2α correlates with poor patient prognosis in epithelial ovarian cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Janeen (Verified Customer) (07-22-2021)
There are some background bands at high contrast. But overall, a nice clean signal at a contrast level appropriate for interpretation.