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  • KD/KO Validated

CSNK2A1 Polyclonal antibody

CSNK2A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10992-1-AP

Synonyms

Casein kinase II subunit alpha, CK II alpha, CK2a, CK2A1, CK2α, CKII, CSNK2A1



Tested Applications

Positive WB detected inPC-3 cells, A431 cells, NIH/3T3 cells, K-562 cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10992-1-AP targets CSNK2A1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSNK2A1 fusion protein Ag1452
Full Name casein kinase 2, alpha 1 polypeptide
Calculated molecular weight 45 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC011668
Gene symbol CSNK2A1
Gene ID (NCBI) 1457
RRIDAB_2245531
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.

Protocols

Product Specific Protocols
WB protocol for CSNK2A1 antibody 10992-1-APDownload protocol
IHC protocol for CSNK2A1 antibody 10992-1-APDownload protocol
IF protocol for CSNK2A1 antibody 10992-1-APDownload protocol
IP protocol for CSNK2A1 antibody 10992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Adv Sci (Weinh)

Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy

Authors - Qian Gou
humanWB

Nucleic Acids Res

CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signaling

Authors - Moe Matsumoto
  • KD Validated
humanWB

Nucleic Acids Res

CK2 kinase-mediated PHF8 phosphorylation controls TopBP1 stability to regulate DNA replication.

Authors - Haihua Feng
  • KD Validated
humanWB

Nucleic Acids Res

ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters.

Authors - Kihyun Park
mouseWB

Redox Biol

Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.

Authors - Ming Kong
humanWB

Oncogene

β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.

Authors - Yanwei Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Janeen (Verified Customer) (07-22-2021)

There are some background bands at high contrast. But overall, a nice clean signal at a contrast level appropriate for interpretation.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: prostate/ RWPE-1
CSNK2A1 Antibody Western Blot validation (1:5000 dilution) in prostate/ RWPE-1 (Cat no:10992-1-AP)