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- KD/KO Validated
CSNK2A1 Polyclonal antibody
CSNK2A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, CoIP, ELISA
Cat no : 10992-1-AP
Validation Data Gallery
|Positive WB detected in||PC-3 cells, A431 cells, NIH/3T3 cells, K-562 cells, Raji cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 15 publications below|
|IHC||See 1 publications below|
|IF||See 1 publications below|
|IP||See 2 publications below|
|CoIP||See 1 publications below|
10992-1-AP targets CSNK2A1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CSNK2A1 fusion protein Ag1452|
|Full Name||casein kinase 2, alpha 1 polypeptide|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||40-45 kDa|
|GenBank accession number||BC011668|
|Gene ID (NCBI)||1457|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.
|Product Specific Protocols|
|WB protocol for CSNK2A1 antibody 10992-1-AP||Download protocol|
|IHC protocol for CSNK2A1 antibody 10992-1-AP||Download protocol|
|IF protocol for CSNK2A1 antibody 10992-1-AP||Download protocol|
|IP protocol for CSNK2A1 antibody 10992-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Nucleic Acids Res
CK2 kinase-mediated PHF8 phosphorylation controls TopBP1 stability to regulate DNA replication.
Nucleic Acids Res
ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters.
Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.
β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.
J Biol Chem
Structural insights into multifunctionality of human FACT complex subunit hSSRP1
J Transl Med
NCAPG promotes the proliferation of hepatocellular carcinoma through the CKII-dependent regulation of PTEN.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Janeen (Verified Customer) (07-22-2021)
There are some background bands at high contrast. But overall, a nice clean signal at a contrast level appropriate for interpretation.