CSNK2A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CSNK2A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

22

Reviews (1)

Cat no : 10992-1-AP

Synonyms

Casein kinase II subunit alpha, CK II alpha, CK2a, CK2A1, CK2α, CKII, CSNK2A1

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Validation Data Gallery

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  WB analysis of PC-3 using 10992-1-AP

  WB result of CSNK2A1 antibody (10992-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSNK2A1 transfected PC-3 cells.

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  WB analysis using 10992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10992-1-AP (CSNK2A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10992-1-AP (CSNK2A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10992-1-AP (CSNK2A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10992-1-AP (CSNK2A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10992-1-AP

  IP result of anti-CSNK2A1 (IP:10992-1-AP, 4ug; Detection:10992-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human breast cancer using 10992-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10992-1-AP (CSNK2A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10992-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10992-1-AP (CSNK2A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10992-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10992-1-AP (CSNK2A1 antibody) at dilution of 1:200 (under 10x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9).

• 

  IHC staining of human colon cancer using 10992-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10992-1-AP (CSNK2A1 antibody) at dilution of 1:200 (under 40x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9).

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  IF Staining of HeLa using 10992-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10992-1-AP (CSNK2A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Phalloidine stains the F-actin.

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Tested Applications

• Positive WB detected in: PC-3 cells, A431 cells, NIH/3T3 cells, K-562 cells, Raji cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

10992-1-AP targets CSNK2A1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSNK2A1 fusion protein Ag1452
• Full Name: casein kinase 2, alpha 1 polypeptide
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC011668
• Gene Symbol: CSNK2A1
• Gene ID (NCBI): 1457
• RRID: AB_2245531
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK2A1(Casein kinase II subunit alpha) is also named as CK2A1 and belongs to the CK2 subfamily. The CSNK2A1 gene contains 8 exons whose sequences comprise bases 102 to 824 of the coding region of the human casein kinase II alpha subunit. Casein kinase II is a ubiquitous protein-serine/threonine kinase in eukaryotic cells that catalyzes the phosphorylation of many protein substrates and has been regarded as a second-messenger-independent protein kinase. This enzyme is composed of an α subunit (37 to 44kDa) and a β subunit (24 to 28 kDa) and exists as an α2β2 tetramer (PMID:1448100). Casein kinase 2 has been found to be increased at protein level and up-regulated at the level of enzyme activity in the majority of cancers. However, elevated levels of casein kinase 2 are present in certain normal organs such as brain and testes.

Publications

Species	Application	Title
human	WB,IHC	Adv Sci (Weinh); Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy; Authors - Qian Gou
human	WB	Nucleic Acids Res; CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signaling; Authors - Moe Matsumoto ; KD Validated
human	WB	Nucleic Acids Res; CK2 kinase-mediated PHF8 phosphorylation controls TopBP1 stability to regulate DNA replication.; Authors - Haihua Feng ; KD Validated
human	WB	Nucleic Acids Res; ZWC complex-mediated SPT5 phosphorylation suppresses divergent antisense RNA transcription at active gene promoters.; Authors - Kihyun Park
mouse	WB	Redox Biol; Redox-sensitive activation of CCL7 by BRG1 in hepatocytes during liver injury.; Authors - Ming Kong
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Janeen (Verified Customer) (07-22-2021)

There are some background bands at high contrast. But overall, a nice clean signal at a contrast level appropriate for interpretation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: prostate/ RWPE-1

CSNK2A1 Antibody Western Blot validation (1:5000 dilution) in prostate/ RWPE-1 (Cat no:10992-1-AP)