CTCF Polyclonal antibody

CTCF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10915-1-AP

Synonyms

CTCFL paralog, CCCTC-binding factor, CCCTC binding factor, 11-zinc finger protein, 11 zinc finger protein

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Validation Data Gallery

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  WB analysis of human brain using 10915-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10915-1-AP (CTCF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 10915-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10915-1-AP (CTCF Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of 4T1 using 10915-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 10915-1-AP (CTCF Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IP experiment of MCF-7 using 10915-1-AP

  IP result of anti-CTCF (IP:10915-1-AP, 5ug; Detection:10915-1-AP 1:300) with MCF-7 cells lysate 2560ug.

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  IHC staining of human lymphoma using 10915-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10915-1-AP (CTCF antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 10915-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTCF antibody (10915-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: human brain tissue, 4T1 cells, HEK-293T cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Product Information

10915-1-AP targets CTCF in WB, IHC, IF/ICC, IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CTCF fusion protein Ag1340
• Full Name: CCCTC-binding factor (zinc finger protein)
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 55-100 kDa, 130-150 kDa
• GenBank Accession Number: BC014267
• Gene Symbol: CTCF
• Gene ID (NCBI): 10664
• RRID: AB_2086799
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcriptional insulators are DNA elements that set boundaries on the actions of enhancer and silencer elements and thereby organize the eukaryotic genome into regulatory domains. All vertebrate insulators appear to use the versatile CTCF protein. CTCF uses various combinations of its 11 zinc fingers to recognize a variety of unrelated DNA sequences. Once bound to DNA, CTCF can function as a transcriptional insulator, repressor, or activator, depending on the context of the binding site [PMID:12787766,15454938]. In vertebrates, this 11 zinc-finger protein is shown to be crucial in processes of epigenetic imprinting, X chromosome inactivation , and associated with various complex human diseases including cancer and diabetes [PMID:23139640]. The calcualted molecular weight of CTCF is 83 kDa, but stimulation of human corneal epithelial cells with hypoxic stress suppressed a high molecular mass form of CTCF (150 kDa), but not a lower molecular weight form of CTCF (130 kDa)(PMID: 22354964), and there are multiple isoforms of CTCF with molecular masses of 55, 70, 73, 80, 97, and 130 kDa have been observed (PMID: 12878173).

Publications

Species	Application	Title
	RIP	Sci Total Environ; Transcriptome sequencing analysis reveals a potential role of lncRNA NONMMUT058932.2 and NONMMUT029203.2 in abnormal myelin development of male offspring following prenatal PM2.5 exposure; Authors - Yanwen Hou
human	WB	Carcinogenesis; Hepatocellular carcinoma risk variant modulates lncRNA HLA-DQB1-AS1 expression via a long-range enhancer-promoter interaction.; Authors - Haoxue Wang
human	ChIP	mBio; A Low-Producing Haplotype of Interleukin-6 Disrupting CTCF Binding Is Protective against Severe COVID-19.; Authors - Tao Chen
human	WB	Cancer Cell Int; CTCF-activated FUCA1 functions as a tumor suppressor by promoting autophagy flux and serum α-L-fucosidase serves as a potential biomarker for prognosis in ccRCC; Authors - Shuo Zhao