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  • KD/KO Validated

CTHRC1 Polyclonal antibody

CTHRC1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16534-1-AP

Synonyms

CTHRC1, Protein NMTC1



Tested Applications

Positive WB detected inA375 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, mouse lung tissue, rat lung tissue
Positive IP detected inA375 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16534-1-AP targets CTHRC1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTHRC1 fusion protein Ag9812
Full Name collagen triple helix repeat containing 1
Calculated molecular weight 243 aa, 26 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC014245
Gene symbol CTHRC1
Gene ID (NCBI) 115908
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Collagen triple helix repeat-containing protein 1 (CTHRC1), also known protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).

Protocols

Product Specific Protocols
WB protocol for CTHRC1 antibody 16534-1-APDownload protocol
IHC protocol for CTHRC1 antibody 16534-1-APDownload protocol
IF protocol for CTHRC1 antibody 16534-1-APDownload protocol
IP protocol for CTHRC1 antibody 16534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Exp Metastasis

CTHRC1 promotes M2-like macrophage recruitment and myometrial invasion in endometrial carcinoma by integrin-Akt signaling pathway.

Authors - Lu-Ying Li
  • KD Validated
humanWB,IF

Respir Res

Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-β1.

Authors - Jin Jin
humanWB

Int J Mol Sci

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Authors - Federica Morani
humanWB

Balkan Med J

miR-509-3p Suppresses Migration, Invasion and Epithelial-Mesenchymal Transition in Melanoma Cells by Targeting Collagen Triple Helix Repeat Containing 1.

Authors - Keyu Yuan
humanWB

Cancer Biother Radiopharm

Lentivirus-Mediated Knockdown of CTHRC1 Inhibits Osteosarcoma Cell Proliferation and Migration.

Authors - Weilin Sang
  • KD Validated

Int J Mol Med

CTHRC1 mediates IL‑1β‑induced apoptosis in chondrocytes via JNK1/2 signaling.

Authors - Qi Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Valeria (Verified Customer) (11-11-2020)

Fixative: 4% paraformaldehydeAntigen retrieval step: NoPermeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)Protein blocking: 10% NGS diluted in TBSTEndogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc)Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, Goat anti-Rabbit isotype IgG, 1:100

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human skeletal muscle sections (10um)
CTHRC1 Antibody Immunofluorescence validation (1:200 dilution) in Human skeletal muscle sections (10um) (Cat no:16534-1-AP)