Validation Data Gallery
|Positive WB detected in||A375 cells, rat lung tissue, mouse lung tissue, HEK-293 cells, Jurkat cells, mouse brain tissue|
|Positive IP detected in||A375 cells|
|Positive IF detected in||A375 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16534-1-AP targets CTHRC1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CTHRC1 fusion protein Ag9812|
|Full Name||collagen triple helix repeat containing 1|
|Calculated molecular weight||243 aa, 26 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC014245|
|Gene ID (NCBI)||115908|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Collagen triple helix repeat-containing protein 1 (CTHRC1), also known as protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).
Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer.
Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-β1.
Int J Mol Sci
Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.
J Cell Sci
miR-9 inhibits Schwann cell migration by targeting CTHRC1 following sciatic nerve injury.
Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro
Int J Biol Sci
NEDD4L-induced β-catenin ubiquitination suppresses the formation and progression of interstitial pulmonary fibrosis via inhibiting the CTHRC1/HIF-1α axis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Valeria (Verified Customer) (11-11-2020)
Fixative: 4% paraformaldehydeAntigen retrieval step: NoPermeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)Protein blocking: 10% NGS diluted in TBSTEndogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc)Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, Goat anti-Rabbit isotype IgG, 1:100