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  • KD/KO Validated

CTHRC1 Polyclonal antibody

CTHRC1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 16534-1-AP


CTHRC1, Protein NMTC1

Tested Applications

Positive WB detected inA375 cells, rat lung tissue, mouse lung tissue, HEK-293 cells, Jurkat cells, mouse brain tissue
Positive IP detected inA375 cells
Positive IF detected inA375 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16534-1-AP targets CTHRC1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTHRC1 fusion protein Ag9812
Full Name collagen triple helix repeat containing 1
Calculated Molecular Weight 243 aa, 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC014245
Gene Symbol CTHRC1
Gene ID (NCBI) 115908
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Collagen triple helix repeat-containing protein 1 (CTHRC1), also known as protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).


Product Specific Protocols
WB protocol for CTHRC1 antibody 16534-1-APDownload protocol
IF protocol for CTHRC1 antibody 16534-1-APDownload protocol
IP protocol for CTHRC1 antibody 16534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer.

Authors - Yumin Li

Respir Res

Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-β1.

Authors - Jin Jin

Int J Mol Sci

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Authors - Federica Morani

J Cell Sci

miR-9 inhibits Schwann cell migration by targeting CTHRC1 following sciatic nerve injury.

Authors - Songlin Zhou
  • KD Validated


Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro

Authors - Yong Feng
  • KD Validated

Int J Biol Sci

NEDD4L-induced β-catenin ubiquitination suppresses the formation and progression of interstitial pulmonary fibrosis via inhibiting the CTHRC1/HIF-1α axis.

Authors - Lin Chen


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Valeria (Verified Customer) (11-11-2020)

Fixative: 4% paraformaldehydeAntigen retrieval step: NoPermeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)Protein blocking: 10% NGS diluted in TBSTEndogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc)Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, Goat anti-Rabbit isotype IgG, 1:100

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human skeletal muscle sections (10um)
CTHRC1 Antibody Immunofluorescence validation (1:200 dilution) in Human skeletal muscle sections (10um) (Cat no:16534-1-AP)