|Positive WB detected in||A375 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, mouse lung tissue, rat lung tissue|
|Positive IP detected in||A375 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A375 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16534-1-AP targets CTHRC1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CTHRC1 fusion protein Ag9812|
|Full Name||collagen triple helix repeat containing 1|
|Calculated molecular weight||243 aa, 26 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC014245|
|Gene ID (NCBI)||115908|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Collagen triple helix repeat-containing protein 1 (CTHRC1), also known protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).
|Product Specific Protocols|
|WB protocol for CTHRC1 antibody 16534-1-AP||Download protocol|
|IHC protocol for CTHRC1 antibody 16534-1-AP||Download protocol|
|IF protocol for CTHRC1 antibody 16534-1-AP||Download protocol|
|IP protocol for CTHRC1 antibody 16534-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Clin Exp Metastasis
CTHRC1 promotes M2-like macrophage recruitment and myometrial invasion in endometrial carcinoma by integrin-Akt signaling pathway.
Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-β1.
Int J Mol Sci
Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.
Balkan Med J
miR-509-3p Suppresses Migration, Invasion and Epithelial-Mesenchymal Transition in Melanoma Cells by Targeting Collagen Triple Helix Repeat Containing 1.
Cancer Biother Radiopharm
Lentivirus-Mediated Knockdown of CTHRC1 Inhibits Osteosarcoma Cell Proliferation and Migration.
Int J Mol Med
CTHRC1 mediates IL‑1β‑induced apoptosis in chondrocytes via JNK1/2 signaling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Valeria (Verified Customer) (11-11-2020)
Fixative: 4% paraformaldehydeAntigen retrieval step: NoPermeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)Protein blocking: 10% NGS diluted in TBSTEndogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc)Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, Goat anti-Rabbit isotype IgG, 1:100