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- KD/KO Validated
CTHRC1 Polyclonal antibody
CTHRC1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, ELISA
Cat no : 16534-1-AP
Validation Data Gallery
|Positive WB detected in||A375 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, mouse lung tissue, rat lung tissue|
|Positive IP detected in||A375 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A375 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 5 publications below|
|WB||See 13 publications below|
|IHC||See 9 publications below|
|IF||See 5 publications below|
16534-1-AP targets CTHRC1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CTHRC1 fusion protein Ag9812|
|Full Name||collagen triple helix repeat containing 1|
|Calculated molecular weight||243 aa, 26 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC014245|
|Gene ID (NCBI)||115908|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Collagen triple helix repeat-containing protein 1 (CTHRC1), also known protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).
|Product Specific Protocols|
|WB protocol for CTHRC1 antibody 16534-1-AP||Download protocol|
|IHC protocol for CTHRC1 antibody 16534-1-AP||Download protocol|
|IF protocol for CTHRC1 antibody 16534-1-AP||Download protocol|
|IP protocol for CTHRC1 antibody 16534-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer.
J Cell Sci
miR-9 inhibits Schwann cell migration by targeting CTHRC1 following sciatic nerve injury.
Int J Biol Sci
NEDD4L-induced β-catenin ubiquitination suppresses the formation and progression of interstitial pulmonary fibrosis via inhibiting the CTHRC1/HIF-1α axis.
Hyperdense Pancreatic Ductal Adenocarcinoma: Clinical Characteristics and Proteomic Landscape.
Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro
Am J Cancer Res
SP1-mediated microRNA-520d-5p suppresses tumor growth and metastasis in colorectal cancer by targeting CTHRC1.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Valeria (Verified Customer) (11-11-2020)
Fixative: 4% paraformaldehydeAntigen retrieval step: NoPermeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)Protein blocking: 10% NGS diluted in TBSTEndogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc)Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, Goat anti-Rabbit isotype IgG, 1:100