Validation Data Gallery
|Positive WB detected in||mouse uterus tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human stomach tissue, human colon tissue, human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12831-1-AP targets Alpha E-Catenin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Alpha E-Catenin fusion protein Ag3565|
|Full Name||catenin (cadherin-associated protein), alpha 1, 102kDa|
|Calculated molecular weight||906 aa, 100 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC031262|
|Gene ID (NCBI)||1495|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.
|Product Specific Protocols|
|WB protocol for Alpha E-Catenin antibody 12831-1-AP||Download protocol|
|IHC protocol for Alpha E-Catenin antibody 12831-1-AP||Download protocol|
|IF protocol for Alpha E-Catenin antibody 12831-1-AP||Download protocol|
|IP protocol for Alpha E-Catenin antibody 12831-1-AP||Download protocol|
|FC protocol for Alpha E-Catenin antibody 12831-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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