Alpha E-Catenin Polyclonal antibody

Alpha E-Catenin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12831-1-AP


alpha catenin, Alpha E catenin, Alpha E-Catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1, α-catenin, α-E-catenin, αE-catenin

Tested Applications

Positive WB detected inmouse uterus tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach tissue, human colon tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12831-1-AP targets Alpha E-Catenin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha E-Catenin fusion protein Ag3565
Full Name catenin (cadherin-associated protein), alpha 1, 102kDa
Calculated Molecular Weight 906 aa, 100 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC031262
Gene Symbol CTNNA1
Gene ID (NCBI) 1495
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.


Product Specific Protocols
WB protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
IHC protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
IF protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
IP protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
FC protocol for Alpha E-Catenin antibody 12831-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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