|Positive WB detected in||human brain tissue, HeLa cells, HEK-293 cells, mouse brain tissue, NIH/3T3 cells, RAW264.7|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12180-1-AP targets p120 Catenin in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||p120 Catenin fusion protein Ag2824|
|Full Name||catenin (cadherin-associated protein), delta 1|
|Calculated molecular weight||948 aa, 105 kDa|
|Observed molecular weight||90-120 kDa|
|GenBank accession number||BC010501|
|Gene ID (NCBI)||1500|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).
|Product Specific Protocols|
|WB protocol for p120 Catenin antibody 12180-1-AP||Download protocol|
|IHC protocol for p120 Catenin antibody 12180-1-AP||Download protocol|
|IF protocol for p120 Catenin antibody 12180-1-AP||Download protocol|
|IP protocol for p120 Catenin antibody 12180-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Exosome-transmitted p120-catenin suppresses hepatocellular carcinoma progression via STAT3 pathways.
Hsa-miR-425-5p promotes tumor growth and metastasis by activating the CTNND1-mediated β-catenin pathway and EMT in colorectal cancer.
NDRG2 regulates adherens junction integrity to restrict colitis and tumourigenesis.
EMBO Mol Med
Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy.