Cathepsin A Polyclonal antibody

Cathepsin A Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 15020-1-AP

Synonyms

Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, CTSA, GLB2, GSL, Lysosomal protective protein, NGBE, PPCA, PPGB, Protective protein cathepsin A



Tested Applications

Positive WB detected inA549 cells, Sp2/0 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15020-1-AP targets Cathepsin A in WB, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cathepsin A fusion protein Ag7073
Full Name cathepsin A
Calculated molecular weight 54 kDa
Observed molecular weight 54-60, 32, 20 kDa
GenBank accession numberBC000597
Gene symbol CTSA
Gene ID (NCBI) 5476
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CTSA is also named as PPGB(protective protein for beta-galactosidase), PPCA(protective protein cathepsin A), cathepsin A, carboxypeptidase C, carboxypeptidase L and belongs to the peptidase S10 family. It is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. It can be cleaved into the following 2 chains and defects in CTSA are the cause of galactosialidosis (GSL). This protein has 2 glycosylation sites. CTSA is synthesized as a 54-kDa precursor protein and composed of 32- and 20-kDa subunits linked together by disulfide bonds(PMID: 16461364,19574551).

Protocols

Product Specific Protocols
WB protocol for Cathepsin A antibody 15020-1-APDownload protocol
IHC protocol for Cathepsin A antibody 15020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

Nature

Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.

Authors - Dali Han
humanIHC

Sci Rep

Prognostic significance and oncogene function of cathepsin A in hepatocellular carcinoma.

Authors - Huaxiang Wang
mouseWB

J Hepatol

SNX10 mediates alcohol-induced liver injury and steatosis via regulating chaperone-mediated autophagy activation.

Authors - Yan You