Cathepsin A Polyclonal antibody
Cathepsin A Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, FC, ELISA
Cat no : 15020-1-AP
Validation Data Gallery
|Positive WB detected in||A549 cells, Sp2/0 cells, PC-3 cells, DU 145 cells|
|Positive IHC detected in||human liver tissue, human breast cancer tissue, rat kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:750-1:3000|
|Sample-dependent, check data in validation data gallery|
|WB||See 2 publications below|
|IHC||See 2 publications below|
|FC||See 1 publications below|
15020-1-AP targets Cathepsin A in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cathepsin A fusion protein Ag7073|
|Full Name||cathepsin A|
|Calculated molecular weight||54 kDa|
|Observed molecular weight||54-60, 32, 20 kDa|
|GenBank accession number||BC000597|
|Gene ID (NCBI)||5476|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CTSA is also named as PPGB(protective protein for beta-galactosidase), PPCA(protective protein cathepsin A), cathepsin A, carboxypeptidase C, carboxypeptidase L and belongs to the peptidase S10 family. It is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. It can be cleaved into the following 2 chains and defects in CTSA are the cause of galactosialidosis (GSL). This protein has 2 glycosylation sites. CTSA is synthesized as a 54-kDa precursor protein and composed of 32- and 20-kDa subunits linked together by disulfide bonds(PMID: 16461364,19574551).
|Product Specific Protocols|
|WB protocol for Cathepsin A antibody 15020-1-AP||Download protocol|
|IHC protocol for Cathepsin A antibody 15020-1-AP||Download protocol|
|Click here to view our Standard Protocols|
SNX10 mediates alcohol-induced liver injury and steatosis via regulating chaperone-mediated autophagy activation.
Am J Cancer Res
High cathepsin A protein expression predicts poor prognosis and tumor recurrence of hepatocellular carcinoma patients after curative hepatectomy
Prognostic significance and oncogene function of cathepsin A in hepatocellular carcinoma.
Front Cell Neurosci
Neu1 Is Released From Activated Microglia, Stimulating Microglial Phagocytosis and Sensitizing Neurons to Glutamate.
Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.