Validation Data Gallery
|Positive WB detected in
|A549 cells, Sp2/0 cells, PC-3 cells, DU 145 cells
|Positive IHC detected in
|human liver tissue, human breast cancer tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:3000
|IHC : 1:750-1:3000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
15020-1-AP targets Cathepsin A in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|Cathepsin A fusion protein Ag7073
|Calculated molecular weight
|Observed molecular weight
|54-60, 32, 20 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CTSA is also named as PPGB(protective protein for beta-galactosidase), PPCA(protective protein cathepsin A), cathepsin A, carboxypeptidase C, carboxypeptidase L and belongs to the peptidase S10 family. It is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. It can be cleaved into the following 2 chains and defects in CTSA are the cause of galactosialidosis (GSL). This protein has 2 glycosylation sites. CTSA is synthesized as a 54-kDa precursor protein and composed of 32- and 20-kDa subunits linked together by disulfide bonds(PMID: 16461364,19574551).
SNX10 mediates alcohol-induced liver injury and steatosis via regulating chaperone-mediated autophagy activation.
Am J Cancer Res
High cathepsin A protein expression predicts poor prognosis and tumor recurrence of hepatocellular carcinoma patients after curative hepatectomy
Resistance to abemaciclib is associated with increased metastatic potential and lysosomal protein deregulation in breast cancer cells
Prognostic significance and oncogene function of cathepsin A in hepatocellular carcinoma.
Front Cell Neurosci
Neu1 Is Released From Activated Microglia, Stimulating Microglial Phagocytosis and Sensitizing Neurons to Glutamate.
Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.