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  • KD/KO Validated

CUL3 Polyclonal antibody

CUL3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11107-1-AP

Synonyms

CUL 3, CUL3, cullin 3, KIAA0617



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11107-1-AP targets CUL3 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUL3 fusion protein Ag1555
Full Name cullin 3
Calculated molecular weight 89 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC039598
Gene symbol CUL3
Gene ID (NCBI) 8452
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.

Protocols

Product Specific Protocols
WB protocol for CUL3 antibody 11107-1-APDownload protocol
IHC protocol for CUL3 antibody 11107-1-APDownload protocol
IF protocol for CUL3 antibody 11107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Sci Food Agric

l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.

Authors - Zhengxuan Wang
humanWB

Cell Rep

The Deubiquitinating Enzyme Ataxin-3 Regulates Ciliogenesis and Phagocytosis in the Retina.

Authors - Vasileios Toulis
humanWB

Aging (Albany NY)

Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma.

Authors - Xiao Dong
  • KD Validated
ratWB

J Food Biochem

Rice protein stimulates endogenous antioxidant response attributed to methionine availability in growing rats.

Authors - Zhengxuan Wang
ratWB

J Med Food

Rice Protein Exerts Endogenous Antioxidant Capacity via Methionine Sulfoxide Reductase and the Nrf2 Antioxidant System Independent of Age.

Authors - Hui Li
mouseWB,IHC

J Cancer

Arsenic trioxide (ATO) induced degradation of Cyclin D1 sensitized PD-1/PD-L1 checkpoint inhibitor in oral and esophageal squamous cell carcinoma.

Authors - Guanxia Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Brittney (Verified Customer) (11-26-2018)

10ug load of protein. Cells lysed in RIPA buffer then added to 2X Laemmli buffer for denaturing. Heated at 95deg for 10 minutes as well.Multiple bands, but all follow same pattern. Assumed the band at ~75 kD was the correct one.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HK-2 Cells