CUL4B Monoclonal antibody

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  WB analysis using 60151-1-Ig

  various lysates were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 60151-1-Ig

  WB result of CUL4B antibody (60151-1-Ig, 1:500) with si-Control and si-CUL4B transfected HeLa cells.

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  WB analysis of HeLa using 60151-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 60151-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60151-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 60151-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 60151-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

CUL4B Monoclonal Antibody for WB, IHC, ELISA

Cat No. 60151-1-Ig

Clone No.4E10B10

Publications(5)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

4E10B10, cullin 4B, Cullin-4B, KIAA0695

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, ROS1728 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

60151-1-Ig targets CUL4B in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3563

  Product name: Recombinant human CUL4B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC036216

  Sequence: MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSSNEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSSSSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSSTTVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQNKTIDGIL

• Full Name: cullin 4B
• Calculated Molecular Weight: 913 aa, 104 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC036216
• Gene Symbol: CUL4B
• Gene ID (NCBI): 8450
• RRID: AB_10641034
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13620
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		Curr Biol; Temporal Regulation of ESCO2 Degradation by the MCM Complex, the CUL4-DDB1-VPRBP Complex, and the Anaphase-Promoting Complex.; Authors - Masashi Minamino
		Int J Oncol; miR‑381 and miR‑489 suppress cell proliferation and invasion by targeting CUL4B via the Wnt/β‑catenin pathway in gastric cancer.; Authors - Ziling Fang
mouse	WB	Biochem Biophys Res Commun; Insulin alleviates LPS-induced ARDS via inhibiting CUL4B-mediated proteasomal degradation and restoring expression level of Na,K-ATPase α1 subunit through elevating HCF-1.; Authors - Xue-Ting Huang
human	WB,IHC	Pathol Res Pract; Cullin-4B promotes cell proliferation and invasion through inactivation of p53 signaling pathway in colorectal cancer.; Authors - Min Zhong
human	WB	Sci Adv; NEDDylated Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitors; Authors - Alice Meroni ; KD Validated