• Featured Product
  • KD/KO Validated

CUX1 Polyclonal antibody

CUX1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11733-1-AP

Synonyms

CASP, CCAAT displacement protein, CDP, CDP/Cut, CDP1, Clox, COY1, cut like homeobox 1, CUTL1, CUX, Cux/CDP, CUX1, GOLIM6, Homeobox protein cut like 1, Homeobox protein cux 1, Nbla10317, p100, p110, p200, p75



Tested Applications

Positive WB detected inhuman brain tissue, human liver tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse cortex

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11733-1-AP targets CUX1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUX1 fusion protein Ag2335
Full Name cut-like homeobox 1
Calculated molecular weight 676 aa, 77 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC025422
Gene symbol CUX1
Gene ID (NCBI) 1523
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CUX1 is a transcription factors that regulates a large number of genes and microRNAs involved in multiple cellular processes, such as DNA replication, progression into S phase and later, the spindle assembly checkpoint that controls progression through mitosis. Cux1, a marker of supragranular layers. CUX1 acts by preventing binding of positively-acting CCAAT factors to promoters to mediated its gene reperssion. Also it's a component of nf-munr repressor, and binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. It can represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. CUX1 protein containing three cut repeats and a homeodomain called p200, there are several truncated isoforms that have been identified. These isoforms include a testis-specific isoform containing one cut repeat and the homeodomain called p55. In situ hybridization showed that mRNA for p55 was most abundant in round spermatids. Additional isoforms include p75, a protein similar in structure to p55, derived from the use of an alternate promoter in intron 20 ; the cut alternately spliced protein (CASP), a Golgi protein that contains amino-terminal sequences but none of the cut repeats or homeodomains ; and several other isoforms (p80, p90, p110, and p150) that appear to arise via proteolytic processing ].

Protocols

Product Specific Protocols
WB protocol for CUX1 antibody 11733-1-APDownload protocol
IHC protocol for CUX1 antibody 11733-1-APDownload protocol
IF protocol for CUX1 antibody 11733-1-APDownload protocol
IP protocol for CUX1 antibody 11733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

TUBG1 missense variants underlying cortical malformations disrupt neuronal locomotion and microtubule dynamics but not neurogenesis.

Authors - Ekaterina L Ivanova
humanIHC

J Mol Neurosci

Upregulated Expression of CUX1 Correlates with Poor Prognosis in Glioma Patients: a Bioinformatic Analysis.

Authors - Xiujie Wu
mouseIF

Biochem Biophys Res Commun

Loss of homeoprotein Msx1 and Msx2 leading to athletic and kinematic impairment related to the increasing neural excitability of neurons in aberrant neocortex in mice.

Authors - Bin Yu
mouseIF

Front Neural Circuits

Large-Area Fluorescence and Electron Microscopic Correlative Imaging With Multibeam Scanning Electron Microscopy.

Authors - Shinsuke Shibata
humanWB

J Cell Sci

A trap mutant reveals the physiological client spectrum of TRC40.

Authors - Javier Coy-Vergara
humanWB

Front Oncol

Liquid Biopsy of Extracellular Vesicle-Derived miR-193a-5p in Colorectal Cancer and Discovery of Its Tumor-Suppressor Functions.

Authors - Rui Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Carly (Verified Customer) (09-27-2019)

Used in an antibody array to identify proteins in human EDTA plasma.

  • Applications: Other,
  • Cell Tissue Type: Human EDTA Plasma