CX3CL1 Polyclonal antibody

CX3CL1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10108-2-AP

Synonyms

ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1



Tested Applications

Positive WB detected inPC-3 cells, human brain tissue
Positive IHC detected inhuman small intestine tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

10108-2-AP targets CX3CL1 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CX3CL1 fusion protein Ag0161
Full Name chemokine (C-X3-C motif) ligand 1
Calculated molecular weight 42 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC001163
Gene symbol CX3CL1
Gene ID (NCBI) 6376
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chemokines direct the trafficking of white blood cells in immune surveillance, playing a key role in inflammatory and infectious diseases such as AIDS. The human CX3C chemokine carries the chemokine domain on top of an extended mucin-like stalk. CX3CL1 is synthesized as a 50-75kDa precursor and undergoes glycosylation to yield two mature forms: a 100kDa cell membrane bound form and an 85kDa soluble form which is released from the cell surface.The soluble CX3C chemokine has potent chemoattractant activity for T cells and monocytes, and the cell-surface-bound protein, which is induced by primary proinflammatory signals in activated primary endothelial cells, promotes strong adhesion of those leukocytes.

Protocols

Product Specific Protocols
WB protocol for CX3CL1 antibody 10108-2-APDownload protocol
IHC protocol for CX3CL1 antibody 10108-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Neuropathol Exp Neurol

Immunohistochemical and Molecular Investigations Show Alteration in the Inflammatory Profile of Multiple System Atrophy Brain.

Authors - Aoife P Kiely
humanWB,IHC

Cell Biochem Funct

Fractalkine/CX3CR1 induces apoptosis resistance and proliferation through the activation of the AKT/NF-κB cascade in pancreatic cancer cells.

Authors - Hui Wang
humanWB

Mol Oncol

Platelets are recruited to hepatocellular carcinoma tissues in a CX3CL1-CX3CR1 dependent manner and induce tumour cell apoptosis.

Authors - Shuo Miao
humanIHC

J Pathol

Current smoking-specific gene expression signature in normal bronchial epithelium is enhanced in squamous cell lung cancer.

Authors - Boelens Mirjam C MC
humanIHC

Blood

Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.

Authors - Ma Yue Y