CX3CR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CX3CR1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA

Conjugate

Unconjugated

Publications

23

Reviews (1)

Cat no : 13885-1-AP

Synonyms

Beta chemokine receptor like 1, C X3 C CKR 1, CCRL1, CMK BRL 1, CMK BRL1, CMKBRL1, CMKDR1, CX3C chemokine receptor 1, CX3CR1, Fractalkine receptor, G protein coupled receptor 13, GPR13, GPRV28, V28

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Validation Data Gallery

• 

  WB analysis using 13885-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13885-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 13885-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 13885-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13885-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 13885-1-AP

  IP Result of anti-CX3CR1 (IP:13885-1-AP, 4ug; Detection:13885-1-AP 1:1000) with SH-SY5Y cells lysate 1600ug.

• 

  IHC staining of human tonsillitis using 13885-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 13885-1-AP (CX3CR1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 13885-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 13885-1-AP (CX3CR1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 13885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13885-1-AP (CX3CR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13885-1-AP (CX3CR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of K-562 using 13885-1-AP

  1X10^6 K-562 cells were stained with 0.2ug CX3CR1 antibody (13885-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HeLa cells, HL-60 cells, K-562 cells, SH-SY5Y cells, U-937 cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human tonsillitis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

13885-1-AP targets CX3CR1 in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CX3CR1 fusion protein Ag4149
• Full Name: chemokine (C-X3-C motif) receptor 1
• Calculated molecular weight: 40 kDa
• Observed molecular weight: 44-47 kDa
• GenBank accession number: BC028078
• Gene symbol: CX3CR1
• Gene ID (NCBI): 1524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CX3CR1, also known as GPR13, V28 and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed on microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12).

Publications

Species	Application	Title
mouse	IP	Brain; Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.; Authors - Yun Xia
mouse	WB	J Control Release; Chemoattractants driven and microglia based biomimetic nanoparticle treating TMZ-resistant glioblastoma multiforme.; Authors - Sai Qiao
mouse	WB	Nanoscale; Calming egress of inflammatory monocytes and related septic shock by therapeutic CCR2 silencing using macrophage-derived extracellular vesicles.; Authors - Liang Ding
human	WB	Mol Oncol; Platelets are recruited to hepatocellular carcinoma tissues in a CX3CL1-CX3CR1 dependent manner and induce tumour cell apoptosis.; Authors - Shuo Miao
mouse	WB,Cell treatment	J Nanobiotechnology; Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM.; Authors - Bao Liu
mouse	WB,IHC	Int Immunopharmacol; Baicalein ameliorates Alzheimer's disease via orchestration of CX3CR1/NF-κB pathway in a triple transgenic mouse model; Authors - Xin-Mei Xie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryan (Verified Customer) (06-15-2018)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: zebrafish retina

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