Tested Applications
| Positive WB detected in | HeLa cells, SH-SY5Y cells, THP-1 cells, Caco-2 cells |
| Positive IF-P detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29819-1-AP targets CX3CR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27698 Product name: Recombinant human CX3CR1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 56-231 aa of BC028078 Sequence: LTNSKKPKSVTDIYLLNLALSDLLFVATLPFWTHYLINEKGLHNAMCKFTTAFFFIGFFGSIFFITVISIDRYLAIVLAANSMNNRTVQHGVTISLGVWAAAILVAAPQFMFTKQKENECLGDYPEVLQEIWPVLRNVETNFLGFLLPLLIMSYCYFRIIQTLFSCKNHKKAKAIK Predict reactive species |
| Full Name | chemokine (C-X3-C motif) receptor 1 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 40 kDa, 44 kDa |
| GenBank Accession Number | BC028078 |
| Gene Symbol | CX3CR1 |
| Gene ID (NCBI) | 1524 |
| RRID | AB_3086173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49238 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CX3CR1, also known as GPR13, V28 and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed on microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12). The two CX3CR1 bands exhibited can be attributed to various factors (PMID: 30628679).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CX3CR1 antibody 29819-1-AP | Download protocol |
| WB protocol for CX3CR1 antibody 29819-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CX3CR1 Polyclonal antibody (Cat# 29819-1-AP) has 6 citations published in J Control Release, Burns Trauma, Front Immunol, Ann Med, Sci Rep, and Neurogastroenterol Motil. Research fields span bone repair, lung injury and inflammation, HIV reservoir profiling, osteoarthritis biomarkers, gastric cancer progression, and gut microbiota–related intestinal motility. Applications cited include WB, IHC, and IF across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
J Control Release Dentin tubules as a long-term sustained release carrier to accelerate bone repair by loading FTY720 | ||
Burns Trauma Gut microbiota metabolite butyric acid alleviated Klebsiella Pneumoniae induced lung injury by regulating CX3CR1(+)NK via PI3K/AKT pathway. | ||
Front Immunol Integrated transcriptomic profiling of rectal and adipose HIV-1 reservoirs relative to matched PBMCs in ART-treated individuals. | ||
Sci Rep Chronic inflammation promotes gastric cancer progression via ADAM10-mediated cleavage of CX3CL1. | ||
Ann Med Experimental study on the role and biomarker potential of CX3CR1 in osteoarthritis | ||
Neurogastroenterol Motil Lactobacillus reuteri DSM 17938 Improves Intestinal Motility in Mice With Postoperative Ileus by Modulating Gut Microbiota and Macrophage. |
