CX3CR1 Polyclonal antibody

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  WB analysis of HEK-293 using 13885-1-AP

  WB result of CX3CR1 antibody (13885-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CX3CR1 transfected HEK-293 cells.

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  WB analysis using 13885-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 13885-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 13885-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 13885-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13885-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13885-1-AP (CX3CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 13885-1-AP

  IP result of anti-CX3CR1 (IP:13885-1-AP, 4ug; Detection:13885-1-AP 1:1000) with SH-SY5Y cells lysate 1600ug.

• Featured Product
• KD/KO Validated

CX3CR1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 13885-1-AP

Publications(41)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA, Cell treatment

Synonyms

CMK BRL1, CMK BRL 1, CCRL1, C X3 C CKR 1, Beta chemokine receptor-like 1

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Tested Applications

• Positive WB detected in: HeLa cells, HL-60 cells, HEK-293 cells, SH-SY5Y cells, U-937 cells, K-562 cells
• Positive IP detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 28 publications below
• IHC: See 16 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

13885-1-AP targets CX3CR1 in WB, IHC, IF, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CX3CR1 fusion protein Ag4149
• Full Name: chemokine (C-X3-C motif) receptor 1
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 44-47 kDa
• GenBank Accession Number: BC028078
• Gene Symbol: CX3CR1
• Gene ID (NCBI): 1524
• RRID: AB_2087418
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49238
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CX3CR1, also known as GPR13, V28 and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed on microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12).

Publications

Species	Application	Title
mouse	IP	Brain; Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.; Authors - Yun Xia
human	WB,IF	Tissue Cell; Analysis of Aβ-induced neurotoxicity and microglial responses in simple two- and three-dimensional human iPSC-derived cortical culture systems; Authors - Mayuko Takata
mouse	WB	J Control Release; Chemoattractants driven and microglia based biomimetic nanoparticle treating TMZ-resistant glioblastoma multiforme.; Authors - Sai Qiao
mouse	IHC	Front Immunol; Myeloid-derived growth factor promotes M2 macrophage polarization and attenuates Sjögren's syndrome via suppression of the CX3CL1/CX3CR1 axis; Authors - Zi Yang
mouse	WB	Nanoscale; Calming egress of inflammatory monocytes and related septic shock by therapeutic CCR2 silencing using macrophage-derived extracellular vesicles.; Authors - Liang Ding
human	WB	Mol Oncol; Platelets are recruited to hepatocellular carcinoma tissues in a CX3CL1-CX3CR1 dependent manner and induce tumour cell apoptosis.; Authors - Shuo Miao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

Cx3cr1 antibody (in red) tried on human paraffin brain sections, apparently vesicular while marking my microglia.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

CX3CR1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:13885-1-AP)

FH

Ryan (Verified Customer) (06-15-2018)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: zebrafish retina