CXCL10/IP10 Polyclonal antibody

CXCL10/IP10 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10937-1-AP

Synonyms

C X C motif chemokine 10, C7, crg 2, CXCL10, Gamma IP10, gIP 10, IFI10, INP10, IP 10, IP10, mob 1, SCYB10, Small inducible cytokine B10

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Validation Data Gallery

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  IHC staining of human nasopharyngeal carcinoma using 10937-1-AP

  Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 10937-1-AP (CXCL10/IP10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human nasopharyngeal carcinoma using 10937-1-AP

  Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 10937-1-AP (CXCL10/IP10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human nasopharyngeal carcinoma tissue, human ovary tumor tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

10937-1-AP targets CXCL10/IP10 in WB, IHC,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXCL10/IP10 fusion protein Ag1369
• Full Name: chemokine (C-X-C motif) ligand 10
• Calculated molecular weight: 11 kDa
• GenBank accession number: BC010954
• Gene symbol: CXCL10
• Gene ID (NCBI): 3627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CXCL10 (also known as IP-10) is a member of the CXC chemokine family which binds to the CXCR3 receptor to exert its biological effects. CXCL10 is a 12-kDa protein and constitutes two internal disulfide cross bridges. The predicted signal peptidase cleavage generates a 10-kDa secreted polypeptide with four conserved cysteine residues in the N-terminal. The CXCL10 gene localizes on chromosome 4 at band q21, a locus associated with an acute monocytic/B-lymphocyte lineage leukemia exhibiting translocation of t (4; 11) (q21; q23). CXCL10 mediates leukocyte trafficking, adaptive immunity, inflammation, haematopoiesis and angiogenesis. Under proinflammatory conditions CXCL10 is secreted from a variety of cells, such as leukocytes, activated neutrophils, eosinophils, monocytes, epithelial cells, endothelial cells, stromal cells (fibroblasts) and keratinocytes in response to IFN-γ.

Publications

Species	Application	Title
human	IHC	J Gastroenterol Hepatol; TA allele of rs2070673 in the CYP2E1 gene is associated with lobular inflammation and nonalcoholic steatohepatitis in patients with biopsy-proven nonalcoholic fatty liver disease.; Authors - Hong-Lei Ma
mouse,human	WB	J Agric Food Chem; Diosmetin Ameliorates Nonalcoholic Steatohepatitis through Modulating Lipogenesis and Inflammatory Response in a STAT1/CXCL10-Dependent Manner.; Authors - Nanxuan Luo ; KD Validated
rat		Front Immunol; C-X-C Motif Chemokine 10 Contributes to the Development of Neuropathic Pain by Increasing the Permeability of the Blood-Spinal Cord Barrier.; Authors - Hao-Ling Li