Anti-Human CXCL4/PF4 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of human PBMCs using 98410-3-RR (same clone as 98410-3-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL4/PF4 Rabbit RecAb (98410-3-RR, Clone: 243087B12) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98410-3-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98410-3-RR (same clone as 98410-3-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL4/PF4 Rabbit RecAb (98410-3-RR, Clone: 243087B12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated. This data was developed using the same antibody clone with 98410-3-PBS in a different storage buffer formulation.

CXCL4/PF4 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98410-3-PBS

Clone No.243087B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CXCL4, PF4, SCYB4, C X C motif chemokine 4, C-X-C motif chemokine 4

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Product Information

98410-3-PBS targets CXCL4/PF4 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CXCL4/PF4 fusion protein Eg2129
• Full Name: platelet factor 4
• Calculated Molecular Weight: 11 kDa
• GenBank Accession Number: NM_002619.4
• Gene Symbol: PF4
• Gene ID (NCBI): 5196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02776
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CXCL4, also known as platelet factor 4 (PF-4), is the oldest member of the chemokine family. CXCL4 is a chemokine produced by activated platelets and immune cells involved in pathological conditions such as cancer, infections and inflammatory diseases like systemic sclerosis (SSc), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), among others. CXCL4 is present in the α-granules of platelets in amounts that constitute up to 2% platelet protein mass. CXCL4 plays a determinant role in distinct physiological processes such as in hematopoiesis, angiogenesis, coagulation and modulation of immune responses. For instance, CXCL4 can prevent monocyte apoptosis and promote cell survival, induces the production of TNF and reactive oxygen species (ROS) and promotes monocyte differentiation into a unique macrophage-like phenotype.