CXCL8/IL-8 Polyclonal antibody

CXCL8/IL-8 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17038-1-AP

Synonyms

C X C motif chemokine 8, CXCL8, CXCL8/IL-8, Emoctakin, GCP 1, GCP/IL 8 protein I, GCP/IL 8 protein II, GCP/IL 8 protein III, GCP/IL 8 protein IV, GCP/IL 8 protein V, GCP/IL 8 protein VI, GCP1, IL 8, IL8, IL-8, interleukin 8, LYNAP, MDNCF



Tested Applications

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells
Positive IHC detected inhuman tonsillitis tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17038-1-AP targets CXCL8/IL-8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCL8/IL-8 fusion protein Ag10552
Full Name interleukin 8
Calculated molecular weight 99 aa, 11 kDa
GenBank accession numberBC013615
Gene symbol IL8
Gene ID (NCBI) 3576
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.

Protocols

Product Specific Protocols
WB protocol for CXCL8/IL-8 antibody 17038-1-APDownload protocol
IHC protocol for CXCL8/IL-8 antibody 17038-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

RIPK3 activation induces TRIM28 derepression in cancer cells and enhances the anti-tumor microenvironment.

Authors - Han-Hee Park
human

EMBO Rep

LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.

Authors - Xuedan Sun
humanWB,IHC,IF

Cell Death Discov

Endometrial stromal cell ferroptosis promotes angiogenesis in endometriosis.

Authors - Guojing Li
humanWB

Cell Death Dis

Necroptosis promotes cell-autonomous activation of proinflammatory cytokine gene expression.

Authors - Kezhou Zhu
humanIHC

J Neurooncol

Radiotherapy induces an immediate inflammatory reaction in malignant glioma: a clinical microdialysis study.

Authors - Pedram Tabatabaei
humanIHC

Cancer Med

Molecular crosstalk between cancer cells and tumor microenvironment components suggests potential targets for new therapeutic approaches in mobile tongue cancer.

Authors - Dayan Dan D