CXCL8/IL-8 Polyclonal antibody

CXCL8/IL-8 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27095-1-AP

Synonyms

(Ala IL 8)77, C X C motif chemokine 8, CXCL8, CXCL8,IL8, CXCL8/IL-8, Emoctakin, GCP 1, GCP/IL 8 protein I, GCP/IL 8 protein II, GCP/IL 8 protein III, GCP/IL 8 protein IV, GCP/IL 8 protein V, GCP/IL 8 protein VI, GCP1, IL 8, IL 8(1 77), IL8, IL-8, IL8/NAP1 form I Interleukin 8, IL8/NAP1 form II, IL8/NAP1 form VI, interleukin 8, MDNCF, MDNCF b IL 8(5 77) IL 8(6 77), MDNCF c, MONAP, NAF, NAF IL 8(7 77), NAP 1, NAP1, Neutrophil activating factor, Protein 3 10C



Tested Applications

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27095-1-AP targets CXCL8/IL-8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, zebrafish, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCL8/IL-8 fusion protein Ag25640
Full Name interleukin 8
Calculated molecular weight 99 aa, 11 kDa
GenBank accession numberBC013615
Gene symbol IL8
Gene ID (NCBI) 3576
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression. The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa).

Protocols

Product Specific Protocols
WB protocol for CXCL8/IL-8 antibody 27095-1-APDownload protocol
IHC protocol for CXCL8/IL-8 antibody 27095-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gut Microbes

Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway

Authors - Jian-Wei Zhang
humanIF

Acta Pharmacol Sin

Calcipotriol abrogates cancer-associated fibroblast-derived IL-8-mediated oxaliplatin resistance in gastric cancer cells via blocking PI3K/Akt signaling.

Authors - Zhen-Xiong Zhao
WB

Int Immunopharmacol

Aflatoxin B1 exposure triggers inflammation and premature skin aging via ERMCS/Ca2+/ROS signaling cascade

Authors - Deyi Wu
IHC

Cancers (Basel)

PKCθ Regulates Pituitary Adenoma Bone Invasion by Activating Osteoclast in NF-κB/IL-1β-Dependent Manner

Authors - Quanji Wang
humanIF

Front Cell Dev Biol

EZH2-Inhibited MicroRNA-454-3p Promotes M2 Macrophage Polarization in Glioma.

Authors - Bin Qi
humanWB

Front Immunol

Single-Cell Transcriptome Analysis of Chronic Antibody-Mediated Rejection After Renal Transplantation.

Authors - Fanhua Kong