CXCL8/IL-8 Antibody

CXCL8/IL-8 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27095-1-AP

Synonyms

C X C motif chemokine 8, CXCL8, CXCL8,IL8, Emoctakin, GCP 1, GCP1, IL 8, IL 8(1 77), IL8, IL-8, IL8/NAP1 form I Interleukin 8, IL8/NAP1 form II, IL8/NAP1 form VI, interleukin 8, MDNCF, MDNCF b IL 8(5 77) IL 8(6 77), NAF, NAF IL 8(7 77), NAP 1, NAP1, Neutrophil activating factor, Protein 3 10C



Tested Applications

Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 27095-1-AP immunogen is Fusion Protein CXCL8/IL-8 protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCL8/IL-8 fusion protein Ag25640
Full Name interleukin 8
Calculated molecular weight 99aa,11 kDa
Observed molecular weight
GenBank accession number BC013615
Gene symbol IL8
Gene ID (NCBI) 3576
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression. The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa).

Protocols

Product Specific Protocols
IHC protocol for CXCL8/IL-8 antibody 27095-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int J Mol Sci

IL-8 Secreted from M2 Macrophages Promoted Prostate Tumorigenesis via STAT3/MALAT1 Pathway.

Authors - Tingjin Zheng
ratWB

Gastroenterol Res Pract

Antichronic Gastric Ulcer Effect of Zinc-Baicalin Complex on the Acetic Acid-Induced Chronic Gastric Ulcer Rat Model.

Authors - Hui Yang
humanIHC

J Thorac Dis

Retrospective immunohistological study of autopsied lungs in patients with acute exacerbation of interstitial pneumonia managed with extracorporeal membrane oxygenation.

Authors - Yoshiko Kida
humanIHC

Am J Cancer Res

MicroRNA-506 suppresses invasiveness and metastasis of human hepatocellular carcinoma cells by targeting IL8.

Authors - Zhuo Wang
ratIHC

Biomed Pharmacother

Tannic acid ameliorates arsenic trioxide-induced nephrotoxicity, contribution of NF-κB and Nrf2 pathways.

Authors - Weiyue Jin