CXCL8/IL-8 Polyclonal antibody

CXCL8/IL-8 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

30

Cat no : 27095-1-AP

Synonyms

(Ala IL 8)77, C X C motif chemokine 8, CXCL8, CXCL8,IL8, CXCL8/IL-8, Emoctakin, GCP 1, GCP/IL 8 protein I, GCP/IL 8 protein II, GCP/IL 8 protein III, GCP/IL 8 protein IV, GCP/IL 8 protein V, GCP/IL 8 protein VI, GCP1, IL 8, IL 8(1 77), IL8, IL-8, IL8/NAP1 form I Interleukin 8, IL8/NAP1 form II, IL8/NAP1 form VI, interleukin 8, MDNCF, MDNCF b IL 8(5 77) IL 8(6 77), MDNCF c, MONAP, NAF, NAF IL 8(7 77), NAP 1, NAP1, Neutrophil activating factor, Protein 3 10C

---

Validation Data Gallery

• 

  WB analysis using 27095-1-AP

  PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 27095-1-AP (CXCL8/IL-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 27095-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27095-1-AP (CXCL8/IL8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 27095-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27095-1-AP (CXCL8/IL8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27095-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27095-1-AP (CXCL8/IL8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27095-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27095-1-AP (CXCL8/IL8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: PMA, LPS and Brefeldin A treated THP-1 cells
• Positive IHC detected in: human lymphoma tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 16 publications below
• IHC: See 13 publications below
• IF: See 2 publications below

Product Information

27095-1-AP targets CXCL8/IL-8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, zebrafish, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXCL8/IL-8 fusion protein Ag25640
• Full Name: interleukin 8
• Calculated molecular weight: 99 aa, 11 kDa
• GenBank accession number: BC013615
• Gene symbol: IL8
• Gene ID (NCBI): 3576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression. The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa).

Publications

Species	Application	Title
human	WB	Gut Microbes; Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway; Authors - Jian-Wei Zhang
human	IF	Acta Pharmacol Sin; Calcipotriol abrogates cancer-associated fibroblast-derived IL-8-mediated oxaliplatin resistance in gastric cancer cells via blocking PI3K/Akt signaling.; Authors - Zhen-Xiong Zhao
	WB	Int Immunopharmacol; Aflatoxin B1 exposure triggers inflammation and premature skin aging via ERMCS/Ca2+/ROS signaling cascade; Authors - Deyi Wu
	IHC	Cancers (Basel); PKCθ Regulates Pituitary Adenoma Bone Invasion by Activating Osteoclast in NF-κB/IL-1β-Dependent Manner; Authors - Quanji Wang
human	IF	Front Cell Dev Biol; EZH2-Inhibited MicroRNA-454-3p Promotes M2 Macrophage Polarization in Glioma.; Authors - Bin Qi
human	WB	Front Immunol; Single-Cell Transcriptome Analysis of Chronic Antibody-Mediated Rejection After Renal Transplantation.; Authors - Fanhua Kong