|Positive WB detected in||HepG2 cells, Jurkat cells, mouse liver tissue|
|Positive IHC detected in||human liver tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 26756-1-AP is CXCR3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CXCR3 fusion protein Ag25056|
|Full Name||chemokine (C-X-C motif) receptor 3|
|Calculated molecular weight||368 aa, 41 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC034403|
|Gene ID (NCBI)||2833|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CXCR3 (C-X-C chemokine receptor type 3) is a G-protein-coupled seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells. CXCR3 is activated by three related chemokines (CXCL9, CXCL10, and CXCL11) and plays an important role in effector T-cell and NK cell trafficking. CXCR3 has three isoforms termed as CXCR3-A, CXCR3-B and CXCR3-alt with the calculated molecular mass of 41kDa, 46kDa and 29kDa respectively. The apparent molecular mass of CXCR3 detected by some scientists is 70kDa, but 45kDa by some others. (PMID: 16847335, 15150261, 22885102, 19151743)
Br J Pharmacol
Phosphodiesterase-4 inhibition attenuates murine ulcerative colitis via interfering with mucosal immunity.
CXCL4 promoted the production of CD4+CD25+FOXP3+treg cells in mouse sepsis model through regulating STAT5/FOXP3 pathway.
J Cell Sci
Gap junction-mediated MiR-200b on osteogenesis and angiogenesis in coculture between MSCs and HUVECs.