- Featured Product
- KD/KO Validated
CXCR7 Polyclonal antibody
CXCR7 Polyclonal Antibody for FC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 20423-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, Jurkat cells, K-562 cells, mouse thymus tissue |
| Positive IP detected in | HUVEC cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 10 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 20423-1-AP is CXCR7 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CXCR7 fusion protein Ag14247 |
| Full Name | chemokine (C-X-C motif) receptor 7 |
| Calculated molecular weight | 362 aa, 41 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC036661 |
| Gene symbol | CXCR7 |
| Gene ID (NCBI) | 57007 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CXCR7 antibody 20423-1-AP | Download protocol |
| IHC protocol for CXCR7 antibody 20423-1-AP | Download protocol |
| IP protocol for CXCR7 antibody 20423-1-AP | Download protocol |
| FC protocol for CXCR7 antibody 20423-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Basis Dis CXCR7 contributes to the aggressive phenotype of cholangiocarcinoma cells. | ||
Biomed Pharmacother Overexpression of CXCR7 promotes mesenchymal stem cells to repair phosgene-induced acute lung injury in rats. | ||
Front Oncol Saikosaponin A Inhibits Triple-Negative Breast Cancer Growth and Metastasis Through Downregulation of CXCR4.
| ||
J Cell Mol Med Interleukin-1β augments the angiogenesis of endothelial progenitor cells in an NF-κB/CXCR7-dependent manner. | ||
Cell Biol Int The protective effects of umbilical cord-derived endothelial colony forming cells on hepatic veno-occlusive disease.
| ||
J Neuroimmunol Prenatal stress affects viability, activation, and chemokine signaling in astroglial cultures. |
