Validation Data Gallery
|Positive WB detected in||Raji cells, Jurkat cells, K-562 cells, mouse thymus tissue|
|Positive IP detected in||HUVEC cells|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Raji cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
20423-1-AP targets CXCR7 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CXCR7 fusion protein Ag14247|
|Full Name||chemokine (C-X-C motif) receptor 7|
|Calculated molecular weight||362 aa, 41 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC036661|
|Gene ID (NCBI)||57007|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).
|Product Specific Protocols|
|WB protocol for CXCR7 antibody 20423-1-AP||Download protocol|
|IHC protocol for CXCR7 antibody 20423-1-AP||Download protocol|
|IP protocol for CXCR7 antibody 20423-1-AP||Download protocol|
|FC protocol for CXCR7 antibody 20423-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The chemokine receptor CXCR7 interacts with EGFR to promote breast cancer cell proliferation.
SDF-1/CXCR7 Chemokine Signaling is Induced in the Peri-Infarct Regions in Patients with Ischemic Stroke.
Circulating hsa_circ_0072309, acting via the miR-100/ACKR3 pathway, maybe a potential biomarker for the diagnosis, prognosis, and treatment of brain metastasis from non-small-cell lung cancer