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  • KD/KO Validated

CXCR7 Polyclonal antibody

CXCR7 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 20423-1-AP

Synonyms

Chemokine orphan receptor 1, CMKOR1, CXC R7, CXCR 7, CXCR7, G protein coupled receptor 159, GPR159, RDC 1, RDC1



Tested Applications

Positive WB detected inRaji cells, Jurkat cells, K-562 cells, mouse thymus tissue
Positive IP detected inHUVEC cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20423-1-AP is CXCR7 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCR7 fusion protein Ag14247
Full Name chemokine (C-X-C motif) receptor 7
Calculated molecular weight 362 aa, 41 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC036661
Gene symbol CXCR7
Gene ID (NCBI) 57007
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).

Protocols

Product Specific Protocols
WB protocol for CXCR7 antibody 20423-1-APDownload protocol
IHC protocol for CXCR7 antibody 20423-1-APDownload protocol
IP protocol for CXCR7 antibody 20423-1-APDownload protocol
FC protocol for CXCR7 antibody 20423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biochim Biophys Acta Mol Basis Dis

CXCR7 contributes to the aggressive phenotype of cholangiocarcinoma cells.

Authors - Alessandra Gentilini
ratWB

Biomed Pharmacother

Overexpression of CXCR7 promotes mesenchymal stem cells to repair phosgene-induced acute lung injury in rats.

Authors - Yiru Shao
humanWB

Front Oncol

Saikosaponin A Inhibits Triple-Negative Breast Cancer Growth and Metastasis Through Downregulation of CXCR4.

Authors - Ying Wang
  • KD Validated
humanWB

J Cell Mol Med

Interleukin-1β augments the angiogenesis of endothelial progenitor cells in an NF-κB/CXCR7-dependent manner.

Authors - Xia Fan
human,mouseWB,IF,IHC

Cell Biol Int

The protective effects of umbilical cord-derived endothelial colony forming cells on hepatic veno-occlusive disease.

Authors - Lulu Chen
  • KD Validated
ratWB

J Neuroimmunol

Prenatal stress affects viability, activation, and chemokine signaling in astroglial cultures.

Authors - Joanna E Sowa