CXCR7 Polyclonal antibody

• Featured Product
• KD/KO Validated

CXCR7 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 20423-1-AP

Synonyms

Chemokine orphan receptor 1, CMKOR1, CXC R7, CXCR 7, CXCR7, G protein coupled receptor 159, GPR159, RDC 1, RDC1

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Validation Data Gallery

• 

  WB analysis of Raji using 20423-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 20423-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20423-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 20423-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HUVEC using 20423-1-AP

  IP result of anti-CXCR7 (IP:20423-1-AP, 3ug; Detection:20423-1-AP 1:500) with HUVEC cells lysate 1400ug.

• 

  IHC staining of mouse brain using 20423-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20423-1-AP (CXCR7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20423-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20423-1-AP (CXCR7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Raji using 20423-1-AP

  1X10^6 Raji cells were stained with 0.2ug CXCR7 antibody (20423-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: Raji cells, Jurkat cells, K-562 cells, mouse thymus tissue
• Positive IP detected in: HUVEC cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Product Information

20423-1-AP targets CXCR7 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXCR7 fusion protein Ag14247
• Full Name: chemokine (C-X-C motif) receptor 7
• Calculated Molecular Weight: 362 aa, 41 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC036661
• Gene Symbol: CXCR7
• Gene ID (NCBI): 57007
• RRID: AB_10693619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).

Publications

Species	Application	Title
mouse	WB	Ann Rheum Dis; FGFR3 deficiency enhances CXCL12-dependent chemotaxis of macrophages via upregulating CXCR7 and aggravates joint destruction in mice.; Authors - Liang Kuang
human	WB	Front Oncol; Saikosaponin A Inhibits Triple-Negative Breast Cancer Growth and Metastasis Through Downregulation of CXCR4.; Authors - Ying Wang ; KD Validated
human	WB	J Cell Mol Med; Interleukin-1β augments the angiogenesis of endothelial progenitor cells in an NF-κB/CXCR7-dependent manner.; Authors - Xia Fan
human	WB	Mol Cancer; The chemokine receptor CXCR7 interacts with EGFR to promote breast cancer cell proliferation.; Authors - Nicole Salazar ; KD Validated
human	IHC	Aging Dis; SDF-1/CXCR7 Chemokine Signaling is Induced in the Peri-Infarct Regions in Patients with Ischemic Stroke.; Authors - Yu Zhang
human	IHC	Cancer Med; Circulating hsa_circ_0072309, acting via the miR-100/ACKR3 pathway, maybe a potential biomarker for the diagnosis, prognosis, and treatment of brain metastasis from non-small-cell lung cancer; Authors - Xiao-Qiang Zhang

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