Validation Data Gallery
|Positive WB detected in||Raji cells, Jurkat cells, K-562 cells, mouse thymus tissue|
|Positive IP detected in||HUVEC cells|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Raji cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20423-1-AP is CXCR7 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CXCR7 fusion protein Ag14247|
|Full Name||chemokine (C-X-C motif) receptor 7|
|Calculated molecular weight||362 aa, 41 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC036661|
|Gene ID (NCBI)||57007|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).
|Product Specific Protocols|
|WB protocol for CXCR7 antibody 20423-1-AP||Download protocol|
|IHC protocol for CXCR7 antibody 20423-1-AP||Download protocol|
|IP protocol for CXCR7 antibody 20423-1-AP||Download protocol|
|FC protocol for CXCR7 antibody 20423-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochim Biophys Acta Mol Basis Dis
CXCR7 contributes to the aggressive phenotype of cholangiocarcinoma cells.
Cell Death Dis
The proton-activated G protein-coupled receptor GPR4 regulates the development of osteoarthritis via modulating CXCL12/CXCR7 signaling.
Overexpression of CXCR7 promotes mesenchymal stem cells to repair phosgene-induced acute lung injury in rats.
Saikosaponin A Inhibits Triple-Negative Breast Cancer Growth and Metastasis Through Downregulation of CXCR4.
J Cell Mol Med
Interleukin-1β augments the angiogenesis of endothelial progenitor cells in an NF-κB/CXCR7-dependent manner.
Cell Biol Int
The protective effects of umbilical cord-derived endothelial colony forming cells on hepatic veno-occlusive disease.