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CYB5R3 Monoclonal antibody

CYB5R3 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

9C4D10

Cat no : 66022-1-Ig

Synonyms

B5R, CYB5R3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1, Diaphorase 1, NADH cytochrome b5 reductase 3



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human heart tissue, human plasma tissue, L02 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66022-1-Ig is CYB5R3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CYB5R3 fusion protein Ag18096
Full Name cytochrome b5 reductase 3
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC004821
Gene symbol CYB5R3
Gene ID (NCBI) 1727
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

Protocols

Product Specific Protocols
WB protocol for CYB5R3 antibody 66022-1-IgDownload protocol
IHC protocol for CYB5R3 antibody 66022-1-IgDownload protocol
IF protocol for CYB5R3 antibody 66022-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols