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CYC1 Polyclonal antibody

CYC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (3)





Cat no : 10242-1-AP


Complex III subunit 4, Complex III subunit IV, CYC1, cytochrome c 1, UQCR4

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10242-1-AP targets CYC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYC1 fusion protein Ag0291
Full Name cytochrome c-1
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC001006
Gene Symbol CYC1
Gene ID (NCBI) 1537
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYC1, also named as Cytochrome c-1, belongs to the cytochrome c family. Cytochrome c1 is one respiratory subunit of the 11 subunits of the cytochrome bc1 complex of the mitochondrial electron-transfer chain. It mediates the transfer of an electron from Rieske iron-sulfur protein to cytochrome c(PMID:3036122 ). CYC1 mediates apoptosis. In this pathway, a variety of apoptotic stimuli cause CYC1 release from mitochondria, which in turn induces a series of biochemical reactions that result incaspase activation and subsequent cell death.(PMID:15189137) While CYC1 release and DNA fragmentation are unaffected by the noncleavablep75 mutant, mitochondrial morphology of dying cells is maintained, and loss of plasma membrane integrity is delayed(PMID:15186778). The protein migrated as a 35 kd band in SDS page.


Product Specific Protocols
WB protocol for CYC1 antibody 10242-1-APDownload protocol
IHC protocol for CYC1 antibody 10242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Cell Biol

PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.

Authors - Shotaro Saita


Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV.

Authors - Margherita Protasoni

Cell Res

Caspase cleavage of cytochrome c1 disrupts mitochondrial function and enhances cytochrome c release.

Authors - Zhu Yushan Y


UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation

Authors - Brittany A Ahlstedt


Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

Authors - Maxence Le Vasseur

Redox Biol

Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice.

Authors - Manish S Bharadwaj


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Daniel (Verified Customer) (09-16-2019)

The nitrocellulose membrane was blocked with PBS, 5% BSA, w/ Tween-20, yet high levels of background was observed.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse kidney, normoxic (lane1), hypoxic (lane2)
CYC1 Antibody Western Blot, validation (1:1000 dilution) in Mouse kidney, normoxic (lane1), hypoxic (lane2) (Cat no:10242-1-AP)