CYC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYC1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

30

Reviews (1)

Cat no : 10242-1-AP

Synonyms

Complex III subunit 4, Complex III subunit IV, CYC1, cytochrome c 1, UQCR4

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Validation Data Gallery

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  WB analysis of human brain using 10242-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10242-1-AP (CYC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10242-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10242-1-AP (CYC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10242-1-AP (CYC1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10242-1-AP (CYC1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10242-1-AP (CYC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10242-1-AP (CYC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10242-1-AP (CYC1 antibody) at dilution of 1:50 (under 25x lens).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 29 publications below
• IHC: See 2 publications below

Product Information

10242-1-AP targets CYC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, monkey, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYC1 fusion protein Ag0291
• Full Name: cytochrome c-1
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC001006
• Gene Symbol: CYC1
• Gene ID (NCBI): 1537
• RRID: AB_2090144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYC1, also named as Cytochrome c-1, belongs to the cytochrome c family. Cytochrome c1 is one respiratory subunit of the 11 subunits of the cytochrome bc1 complex of the mitochondrial electron-transfer chain. It mediates the transfer of an electron from Rieske iron-sulfur protein to cytochrome c(PMID:3036122 ). CYC1 mediates apoptosis. In this pathway, a variety of apoptotic stimuli cause CYC1 release from mitochondria, which in turn induces a series of biochemical reactions that result incaspase activation and subsequent cell death.(PMID:15189137) While CYC1 release and DNA fragmentation are unaffected by the noncleavablep75 mutant, mitochondrial morphology of dying cells is maintained, and loss of plasma membrane integrity is delayed(PMID:15186778). The protein migrated as a 35 kd band in SDS page.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	EMBO J; Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV.; Authors - Margherita Protasoni
human,mouse	WB	Cell Res; Caspase cleavage of cytochrome c1 disrupts mitochondrial function and enhances cytochrome c release.; Authors - Zhu Yushan Y
human	WB	Elife; Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.; Authors - Maxence Le Vasseur
mouse	WB	Redox Biol; Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice.; Authors - Manish S Bharadwaj
human	WB	Environ Pollut; Mitochondrial respiratory chain dysfunction mediated by ROS is a primary point of fluoride-induced damage in Hepa1-6 cells.; Authors - Hong-Wei Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (09-16-2019)

The nitrocellulose membrane was blocked with PBS, 5% BSA, w/ Tween-20, yet high levels of background was observed.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse kidney, normoxic (lane1), hypoxic (lane2)

CYC1 Antibody Western Blot, validation (1:1000 dilution) in Mouse kidney, normoxic (lane1), hypoxic (lane2) (Cat no:10242-1-AP)