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  • KD/KO Validated

CYC1 Polyclonal antibody

CYC1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10242-1-AP

Synonyms

Complex III subunit 4, Complex III subunit IV, CYC1, cytochrome c 1, UQCR4



Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10242-1-AP targets CYC1 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, monkey, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYC1 fusion protein Ag0291
Full Name cytochrome c-1
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC001006
Gene symbol CYC1
Gene ID (NCBI) 1537
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYC1, also named as Cytochrome c-1, belongs to the cytochrome c family. Cytochrome c1 is one respiratory subunit of the 11 subunits of the cytochrome bc1 complex of the mitochondrial electron-transfer chain. It mediates the transfer of an electron from Rieske iron-sulfur protein to cytochrome c(PMID:3036122 ). CYC1 mediates apoptosis. In this pathway, a variety of apoptotic stimuli cause CYC1 release from mitochondria, which in turn induces a series of biochemical reactions that result incaspase activation and subsequent cell death.(PMID:15189137) While CYC1 release and DNA fragmentation are unaffected by the noncleavablep75 mutant, mitochondrial morphology of dying cells is maintained, and loss of plasma membrane integrity is delayed(PMID:15186778). The protein migrated as a 35 kd band in SDS page.

Protocols

Product Specific Protocols
WB protocol for CYC1 antibody 10242-1-APDownload protocol
IHC protocol for CYC1 antibody 10242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Haematologica

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Authors - Nunziata Maio
humanWB

Cell Rep

NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate.

Authors - Luigi D'Angelo
humanWB,IHC

Dis Markers

CYC1 Predicts Poor Prognosis in Patients with Breast Cancer.

Authors - Yingyan Han
  • KD Validated
humanWB

J Biochem Mol Toxicol

Proteomic analysis of mitochondria from infantile hemangioma endothelial cells treated with sodium morrhuate and its liposomal formulation.

Authors - Tu Jun-Bo JB
WB

J Integr Agric

Critical role of cytochrome c1 and its cleavage in porcine reproductive and respiratory syndrome virus nonstructural protein 4-induced cell apoptosis via interaction with nsp4.

Authors - Feng Zhang
  • KD Validated
humanWB

Biochem Biophys Res Commun

The changes in glucose metabolism and cell proliferation in the kidneys of polycystic kidney disease mini-pig models.

Authors - Xiaoying Lian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniel (Verified Customer) (09-16-2019)

The nitrocellulose membrane was blocked with PBS, 5% BSA, w/ Tween-20, yet high levels of background was observed.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse kidney, normoxic (lane1), hypoxic (lane2)
CYC1 Antibody Western Blot, validation (1:1000 dilution) in Mouse kidney, normoxic (lane1), hypoxic (lane2) (Cat no:10242-1-AP)