Cytoglobin Polyclonal antibody

• Featured Product
• KD/KO Validated

Cytoglobin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13317-1-AP

Synonyms

CYGB, cytoglobin, HGB, Histoglobin, STAP

---

Validation Data Gallery

• 

  WB analysis of mouse heart using 13317-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13317-1-AP (Cytoglobin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13317-1-AP (Cytoglobin antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, human uterus tissue, mouse brain tissue, mouse kidney tissue, mouse lung tissue, mouse small intestine tissue, mouse spleen tissue, mouse stomach tissue, PC-3 cells, rat kidney tissue, Transfected HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human heart tissue, human liver tissue, human prostate cancer tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13317-1-AP targets Cytoglobin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytoglobin fusion protein Ag4161
• Full Name: cytoglobin
• Calculated molecular weight: 190 aa, 21 kDa
• Observed molecular weight: 21-29 kDa
• GenBank accession number: BC029798
• Gene symbol: CYGB
• Gene ID (NCBI): 114757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cytoglobin, also named as Histoglobin, HGb, STAP and CYGB, belongs to the globin family. Cytoglobin may have a protective function during conditions of oxidative stress. It may be involved in intracellular oxygen storage or transfer. It serves as a defensive mechanism against oxidative stress both in vitro and in vivo.(PMID:21224051) Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kd, runs as a 29kd one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.

Publications

Species	Application	Title
human	WB	Vascul Pharmacol; Endothelial cell-induced cytoglobin expression in vascular smooth muscle cells contributes to modulation of nitric oxide.; Authors - Brenda Lilly
mouse	IHC	J Cell Physiol; Integrative proteomics and immunochemistry analysis of the factors in the necrosis and repair in acetaminophen-induced acute liver injury in mice.; Authors - Qin Feng
human	WB	Antioxid Redox Signal; The anti-oxidative role of cytoglobin in podocytes: implications for a role in chronic kidney disease.; Authors - Elisa B Randi
human,mouse,rat	WB,IF	Arterioscler Thromb Vasc Biol; The Hemoglobin Homolog Cytoglobin in Smooth Muscle Inhibits Apoptosis and Regulates Vascular Remodeling.; Authors - Frances L Jourd'heuil ; KD Validated