Tested Applications
| Positive WB detected in | mouse heart tissue, human heart tissue, human uterus tissue, mouse brain tissue, mouse kidney tissue, mouse lung tissue, mouse small intestine tissue, mouse spleen tissue, mouse stomach tissue, PC-3 cells, rat kidney tissue, Transfected HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue, human heart tissue, human prostate cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13317-1-AP targets Cytoglobin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4161 Product name: Recombinant human CYGB protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-190 aa of BC029798 Sequence: MEKVPGEMEIERRERSEELSEAERKAVQAMWARLYASCEDVGVAILVRFFVNFPSAKQYFSQFKHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKVSSVLALVGKAHALKHKVEPVYFKILSGVILEVVAEEFASDFPPETQRAWAKLRGLIYSHVTAAYKEVGWVQQVPNATTPPATLPSSGP Predict reactive species |
| Full Name | cytoglobin |
| Calculated Molecular Weight | 190 aa, 21 kDa |
| Observed Molecular Weight | 21-29 kDa |
| GenBank Accession Number | BC029798 |
| Gene Symbol | Cytoglobin |
| Gene ID (NCBI) | 114757 |
| RRID | AB_2090645 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WWM9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cytoglobin, also named as Histoglobin, HGb, STAP and CYGB, belongs to the globin family. Cytoglobin may have a protective function during conditions of oxidative stress. It may be involved in intracellular oxygen storage or transfer. It serves as a defensive mechanism against oxidative stress both in vitro and in vivo.(PMID:21224051) Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with a calculated MW of 21 kDa, runs as a 29 kDa one in canine retina, kidney, liver, lung, and heart (PMID: 16505036). It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cytoglobin antibody 13317-1-AP | Download protocol |
| IHC protocol for Cytoglobin antibody 13317-1-AP | Download protocol |
| WB protocol for Cytoglobin antibody 13317-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cytoglobin Polyclonal antibody (Cat# 13317-1-AP) has 11 citations published in journals including Redox Biology, Genes & Diseases, Phytomedicine, Arteriosclerosis Thrombosis and Vascular Biology, Antioxidants, Antioxidants & Redox Signaling, Molecular Medicine Reports, Scientific Reports, Journal of Cellular Physiology, Vascular Pharmacology, and STAR Protocols. Research fields span oxidative stress, apoptosis, ferroptosis, vascular remodeling, melanoma, hepatic fibrosis, kidney disease, neonatal brain injury, liver injury, and nitric oxide modulation.
| Species | Application | Title |
|---|---|---|
Redox Biol Cytoglobin inhibits non-thermal plasma-induced apoptosis in melanoma cells through regulation of the NRF2-mediated antioxidant response. | ||
Genes Dis IRP1/ARID3A complex promotes pancreatic cancer chemoresistance by suppressing CYGB-related ferroptosis. | ||
Phytomedicine Glycyrrhizic acid ameliorates hepatic fibrosis by inhibiting oxidative stress via AKR7A2 | ||
Arterioscler Thromb Vasc Biol The Hemoglobin Homolog Cytoglobin in Smooth Muscle Inhibits Apoptosis and Regulates Vascular Remodeling.
| ||
Antioxidants (Basel) Cytoglobin Silencing Promotes Melanoma Malignancy but Sensitizes for Ferroptosis and Pyroptosis Therapy Response
| ||
Antioxid Redox Signal The anti-oxidative role of cytoglobin in podocytes: implications for a role in chronic kidney disease. |
