• Featured Product
  • KD/KO Validated

Cytoglobin Polyclonal antibody

Cytoglobin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13317-1-AP

Synonyms

CYGB, cytoglobin, HGB, Histoglobin, STAP



Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, human uterus tissue, mouse brain tissue, mouse kidney tissue, mouse lung tissue, mouse small intestine tissue, mouse spleen tissue, mouse stomach tissue, PC-3 cells, rat kidney tissue, Transfected HEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human heart tissue, human liver tissue, human prostate cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13317-1-AP targets Cytoglobin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytoglobin fusion protein Ag4161
Full Name cytoglobin
Calculated molecular weight 190 aa, 21 kDa
Observed molecular weight 21-29 kDa
GenBank accession numberBC029798
Gene symbol CYGB
Gene ID (NCBI) 114757
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cytoglobin, also named as Histoglobin, HGb, STAP and CYGB, belongs to the globin family. Cytoglobin may have a protective function during conditions of oxidative stress. It may be involved in intracellular oxygen storage or transfer. It serves as a defensive mechanism against oxidative stress both in vitro and in vivo.(PMID:21224051) Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kd, runs as a 29kd one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.

Protocols

Product Specific Protocols
WB protocol for Cytoglobin antibody 13317-1-APDownload protocol
IHC protocol for Cytoglobin antibody 13317-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Vascul Pharmacol

Endothelial cell-induced cytoglobin expression in vascular smooth muscle cells contributes to modulation of nitric oxide.

Authors - Brenda Lilly
mouseIHC

J Cell Physiol

Integrative proteomics and immunochemistry analysis of the factors in the necrosis and repair in acetaminophen-induced acute liver injury in mice.

Authors - Qin Feng
humanWB

Antioxid Redox Signal

The anti-oxidative role of cytoglobin in podocytes: implications for a role in chronic kidney disease.

Authors - Elisa B Randi
human,mouse,ratWB,IF

Arterioscler Thromb Vasc Biol

The Hemoglobin Homolog Cytoglobin in Smooth Muscle Inhibits Apoptosis and Regulates Vascular Remodeling.

Authors - Frances L Jourd'heuil
  • KD Validated