Cytoglobin Polyclonal antibody

• Featured Product
• KD/KO Validated

Cytoglobin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 13317-1-AP

Synonyms

CYGB, cytoglobin, HGB, Histoglobin, STAP

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Validation Data Gallery

• 

  WB analysis of mouse heart using 13317-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13317-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 13317-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 13317-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13317-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 13317-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 13317-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13317-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13317-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13317-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human uterus using 13317-1-AP

  human uterus tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 13317-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13317-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 13317-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13317-1-AP (Cytoglobin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13317-1-AP (Cytoglobin antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13317-1-AP (Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13317-1-AP (Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13317-1-AP (Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13317-1-AP (Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, human uterus tissue, mouse brain tissue, mouse kidney tissue, mouse lung tissue, mouse small intestine tissue, mouse spleen tissue, mouse stomach tissue, PC-3 cells, rat kidney tissue, Transfected HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human heart tissue, human liver tissue, human prostate cancer tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13317-1-AP targets Cytoglobin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytoglobin fusion protein Ag4161
• Full Name: cytoglobin
• Calculated Molecular Weight: 190 aa, 21 kDa
• Observed Molecular Weight: 21-29 kDa
• GenBank Accession Number: BC029798
• Gene Symbol: Cytoglobin
• Gene ID (NCBI): 114757
• RRID: AB_2090645
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytoglobin, also named as Histoglobin, HGb, STAP and CYGB, belongs to the globin family. Cytoglobin may have a protective function during conditions of oxidative stress. It may be involved in intracellular oxygen storage or transfer. It serves as a defensive mechanism against oxidative stress both in vitro and in vivo.(PMID:21224051) Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kd, runs as a 29kd one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.

Publications

Species	Application	Title
human,mouse,rat	WB,IF	Arterioscler Thromb Vasc Biol; The Hemoglobin Homolog Cytoglobin in Smooth Muscle Inhibits Apoptosis and Regulates Vascular Remodeling.; Authors - Frances L Jourd'heuil ; KD Validated
human	WB	Antioxid Redox Signal; The anti-oxidative role of cytoglobin in podocytes: implications for a role in chronic kidney disease.; Authors - Elisa B Randi
human	WB	Antioxidants (Basel); Cytoglobin Silencing Promotes Melanoma Malignancy but Sensitizes for Ferroptosis and Pyroptosis Therapy Response; Authors - Joey De Backer ; KD Validated
mouse	IHC	J Cell Physiol; Integrative proteomics and immunochemistry analysis of the factors in the necrosis and repair in acetaminophen-induced acute liver injury in mice.; Authors - Qin Feng
human	WB	Mol Med Rep; Intranasal administration of Cytoglobin modifies human umbilical cord‑derived mesenchymal stem cells and improves hypoxic‑ischemia brain damage in neonatal rats by modulating p38 MAPK signaling‑mediated apoptosis.; Authors - Hanhua Yang
human	WB	Vascul Pharmacol; Endothelial cell-induced cytoglobin expression in vascular smooth muscle cells contributes to modulation of nitric oxide.; Authors - Brenda Lilly