CYLD Polyclonal antibody

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  WB analysis using 11110-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11110-1-AP (CYLD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11110-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11110-1-AP (CYLD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11110-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11110-1-AP (CYLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11110-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11110-1-AP (CYLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11110-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11110-1-AP (CYLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11110-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11110-1-AP (CYLD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11110-1-AP

  IP result of anti-CYLD (IP:11110-1-AP, 4ug; Detection:11110-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 11110-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11110-1-AP (CYLD antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 11110-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11110-1-AP (CYLD antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 11110-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11110-1-AP (CYLD antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 11110-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11110-1-AP (CYLD antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 11110-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11110-1-AP (CYLD antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 11110-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11110-1-AP (CYLD antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

CYLD Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11110-1-AP

Publications(24)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CDMT, CYLD, CYLD1, CYLDI, Deubiquitinating enzyme CYLD, EAC, HSPC057, KIAA0849, MFT, MFT1, SBS, TEM, Ubiquitin thiolesterase CYLD, USPL2

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, A431 cells, Jurkat cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue, human brain tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 23 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

11110-1-AP targets CYLD in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYLD fusion protein Ag1598
• Full Name: cylindromatosis (turban tumor syndrome)
• Calculated Molecular Weight: 107 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC012342
• Gene Symbol: CYLD
• Gene ID (NCBI): 1540
• RRID: AB_10915966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQC7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYLD, also named as CYLD1, belongs to the peptidase C67 family. It is the protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. CYLD has endodeubiquitinase activity and plays an important role in the regulation of pathways leading to NF-kappa-B activation. CYLD contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. It is a negative regulator of Wnt signaling. CYLD inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. CYLD plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. It is required for normal cell cycle progress and normal cytokinesis. CYLD inhibits nuclear translocation of NF-kappa-B and plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. It is dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. CYLD negatively regulates TNFRSF11A signaling and osteoclastogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CYLD.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination.; Authors - Guixin Zhu ; KD Validated
human	IHC	Cancer Lett; CYLD deficiency enhances metabolic reprogramming and tumor progression in nasopharyngeal carcinoma via PFKFB3.; Authors - Lingzhi Wang
rat	WB	Arterioscler Thromb Vasc Biol; CYLD Deubiquitinates Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 Contributing to Adventitial Remodeling.; Authors - Bing Yu
human	WB	Sci Total Environ; USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.; Authors - Lei Zhang ; KD Validated
human	WB	iScience; Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4; Authors - Chenggang Zhang
human	WB,IHC	Chem Biol Interact; miR-501 is upregulated in cervical cancer and promotes cell proliferation, migration and invasion by targeting CYLD.; Authors - Jaceline Sanches ; KD Validated