CYP2D6 Polyclonal antibody

CYP2D6 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 17868-1-AP

Synonyms

CPD6, CYP2D, CYP2D@, CYP2D6, CYP2DL1, Cytochrome P450 2D6, P450 DB1, P450C2D, P450DB1



Tested Applications

Positive WB detected inrat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:200-1:600
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17868-1-AP is CYP2D6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP2D6 fusion protein Ag12351
Full Name cytochrome P450, family 2, subfamily D, polypeptide 6
Calculated molecular weight 497 aa, 56 kDa
Observed molecular weight 48-56 kDa
GenBank accession numberBC075023
Gene symbol CYP2D6
Gene ID (NCBI) 1565
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYP2D6(Cytochrome P450 2D6) is also named as CYP2DL1 and belongs to the cytochrome P450 family. It is a monooxygenase that involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. CYP2D6 is responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It has 2 isoforms produced by alternative splicing with the molecular weight of 56 kDa and 50 kDa.

Protocols

Product Specific Protocols
WB protocol for CYP2D6 antibody 17868-1-APDownload protocol
IHC protocol for CYP2D6 antibody 17868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Eur J Pharm Sci

PMID: 30776468

Authors - Vinay R Sonawane
humanWB

Drug Des Devel Ther

Effects of Saikosaponin D on CYP1A2 and CYP2D6 in HepaRG Cells.

Authors - Hongfang Li
humanIHC

Theranostics

Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening.

Authors - Zhenyu Wang