|Positive WB detected in||rat liver tissue, mouse liver tissue|
|Positive IHC detected in||human liver cancer tissue, human liver tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:600|
|Sample-dependent, check data in validation data gallery|
The immunogen of 17868-1-AP is CYP2D6/7 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CYP2D6/7 fusion protein Ag12351|
|Full Name||cytochrome P450, family 2, subfamily D, polypeptide 6|
|Calculated molecular weight||497 aa, 56 kDa|
|Observed molecular weight||48-56 kDa|
|GenBank accession number||BC075023|
|Gene ID (NCBI)||1565|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CYP2D6(Cytochrome P450 2D6) is also named as CYP2DL1 and belongs to the cytochrome P450 family. It is a monooxygenase that involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. CYP2D6 is responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It has 2 isoforms produced by alternative splicing with the molecular weight of 56 kDa and 50 kDa. This antibody can recognize human CYP2D6/7, mouse CYP2D3/4/9/10/22/26, rat CYP2D1/3/4/10/26, due to the high homology.
Eur J Pharm Sci
Silybin enhances the blood concentration of brexpiprazole in rats by downregulating expression of CYP3A4 and CYP2D6.
Drug Metab Pharmacokinet
Cloning, sequence analysis, and tissue expression of marmoset paraoxonase 1.
Drug Des Devel Ther
Effects of Saikosaponin D on CYP1A2 and CYP2D6 in HepaRG Cells.
Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening.