CYP3A4 Polyclonal antibody

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  WB analysis using 18227-1-AP

  Rat liver tissue was subjected to SDS PAGE followed by western blot with 18227-1-AP (CYP3A4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18227-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18227-1-AP (CYP3A4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours (mouse brain tissue and mouse colon tissue are negative control).

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  WB analysis of mouse liver using 18227-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18227-1-AP (CYP3A4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18227-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18227-1-AP (CYP3A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 18227-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18227-1-AP (CYP3A4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 18227-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18227-1-AP (CYP3A4 Antibody) at dilution of 1:200 (under 40x lens).

CYP3A4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18227-1-AP

Publications(66)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Albendazole monooxygenase, Albendazole sulfoxidase, CP33, CP34, CYP3A, CYP3A1, CYP3A11, CYP3A3, CYP3A4, CYPIIIA3, CYPIIIA4, Cytochrome P450 3A3, Cytochrome P450 3A4, Cytochrome P450 HLp, Cytochrome P450 NF 25, Cytochrome P450 PCN1, HLP, NF 25, Nifedipine oxidase, P450C3, P450PCN1, Quinine 3 monooxygenase

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Tested Applications

• Positive WB detected in: rat liver tissue, mouse liver tissue, mouse brain tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 47 publications below
• IHC: See 9 publications below
• IF: See 13 publications below

Product Information

18227-1-AP targets CYP3A4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP3A4 fusion protein Ag13074
• Full Name: cytochrome P450, family 3, subfamily A, polypeptide 4
• Calculated Molecular Weight: 503 aa, 57 kDa
• Observed Molecular Weight: 51-57 kDa
• GenBank Accession Number: BC101631
• Gene Symbol: CYP3A4
• Gene ID (NCBI): 1576
• RRID: AB_2090329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08684
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome P450 3A(CYP3A) belongs to the cytochrome P450 family and the enzymes constitute an important detoxification system that contributes to primary metabolism of more than half of all prescribed medications.CYP3A expression determines impairment of drug absorption and efficient systemic clearance in a tissue-specific manner(PMID:17975676). The highest amounts of drug-metabolizing cytochromes P-450 are found in the liver and intestine, with lower amounts found in other organs, including the kidney, lung, and brain. LPS that has reached the circulation stimulates a TLR4-dependent mechanism in the periphery, evoking a reduction in Cyp3a11 expression and metabolism in the liver (PMID: 15878984). Human CYP3A4 is homologous to five mouse P450 isoforms, namely, Cyp3a11, Cyp3a16, Cyp3a41a, Cyp3a41b, and Cyp3a44 (PMID: 22446519). And CYP3A4 is also the human homologue of rat CYP3A1 and CYP3A2 (PMID: 27546062, PMID: 32874920).

Publications

Species	Application	Title
mouse	WB	Nat Commun; Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions; Authors - Shengjie Fan
mouse	IF	Hepatology; Self-renewal of hepatoblasts under chemically defined conditions by reiterative growth factor and chemical screening.; Authors - Linjie Lv
human	IF	Acta Biomater; Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.; Authors - Kun Du
human	IF	ACS Appl Mater Interfaces; Modeling Colorectal Cancer-Induced Liver Portal Vein Microthrombus on a Hepatic Lobule Chip; Authors - Xiaofang Du
monkey	IF	Int J Biol Sci; Hepatobiliary organoids differentiated from hiPSCs relieve cholestasis-induced liver fibrosis in nonhuman primates; Authors - Hongmei Li
human	IHC	Theranostics; Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening.; Authors - Zhenyu Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (11-30-2022)

Good signal with this antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

CYP3A4 Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:18227-1-AP)