CYP3A4 Polyclonal antibody
CYP3A4 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, IF, ELISA
Cat no : 18227-1-AP
|Positive WB detected in||rat liver tissue, mouse liver tissue, mouse brain tissue|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 30 publications below|
|IHC||See 5 publications below|
|IF||See 7 publications below|
18227-1-AP targets CYP3A4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CYP3A4 fusion protein Ag13074|
|Full Name||cytochrome P450, family 3, subfamily A, polypeptide 4|
|Calculated molecular weight||503 aa, 57 kDa|
|Observed molecular weight||51-57 kDa|
|GenBank accession number||BC101631|
|Gene ID (NCBI)||1576|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Cytochrome P450 3A(CYP3A) belongs to the cytochrome P450 family and the enzymes constitute an important detoxification system that contributes to primary metabolism of more than half of all prescribed medications.CYP3A expression determines impairment of drug absorption and efficient systemic clearance in a tissue-specific manner(PMID:17975676). The murine homolog of human CYP3A4 is CYP3A11 (PMID: 19297428). The highest amounts of drug-metabolizing cytochromes P-450 are found in the liver and intestine, with lower amounts found in other organs, including the kidney, lung, and brain. LPS that has reached the circulation stimulates a TLR4-dependent mechanism in the periphery, evoking a reduction in Cyp3a11 expression and metabolism in the liver (PMID: 15878984).
|Product Specific Protocols|
|WB protocol for CYP3A4 antibody 18227-1-AP||Download protocol|
|IHC protocol for CYP3A4 antibody 18227-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Self-renewal of hepatoblasts under chemically defined conditions by reiterative growth factor and chemical screening.
Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening.
Br J Pharmacol
Is platelet responsiveness to clopidogrel attenuated in overweight or obese patients and why? A reverse translational study in mice.
The polyol pathway is an evolutionarily conserved system for sensing glucose uptake.
NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma
Cell Death Dis
Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
James (Verified Customer) (11-30-2022)
Good signal with this antibody