CYP3A4 Polyclonal antibody

CYP3A4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 18227-1-AP


Albendazole monooxygenase, Albendazole sulfoxidase, CP33, CP34, CYP3A, CYP3A1, CYP3A11, CYP3A3, CYP3A4, CYPIIIA3, CYPIIIA4, Cytochrome P450 3A3, Cytochrome P450 3A4, Cytochrome P450 HLp, Cytochrome P450 NF 25, Cytochrome P450 PCN1, HLP, NF 25, Nifedipine oxidase, P450C3, P450PCN1, Quinine 3 monooxygenase

Tested Applications

Positive WB detected inrat liver tissue, mouse liver tissue, mouse brain tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18227-1-AP targets CYP3A4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP3A4 fusion protein Ag13074
Full Name cytochrome P450, family 3, subfamily A, polypeptide 4
Calculated Molecular Weight 503 aa, 57 kDa
Observed Molecular Weight 51-57 kDa
GenBank Accession NumberBC101631
Gene Symbol CYP3A4
Gene ID (NCBI) 1576
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytochrome P450 3A(CYP3A) belongs to the cytochrome P450 family and the enzymes constitute an important detoxification system that contributes to primary metabolism of more than half of all prescribed medications.CYP3A expression determines impairment of drug absorption and efficient systemic clearance in a tissue-specific manner(PMID:17975676). The highest amounts of drug-metabolizing cytochromes P-450 are found in the liver and intestine, with lower amounts found in other organs, including the kidney, lung, and brain. LPS that has reached the circulation stimulates a TLR4-dependent mechanism in the periphery, evoking a reduction in Cyp3a11 expression and metabolism in the liver (PMID: 15878984). Human CYP3A4 is homologous to five mouse P450 isoforms, namely, Cyp3a11, Cyp3a16, Cyp3a41a, Cyp3a41b, and Cyp3a44 (PMID: 22446519). And CYP3A4 is also the human homologue of rat CYP3A1 and CYP3A2 (PMID: 27546062, PMID: 32874920).


Product Specific Protocols
WB protocol for CYP3A4 antibody 18227-1-APDownload protocol
IHC protocol for CYP3A4 antibody 18227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


James (Verified Customer) (11-30-2022)

Good signal with this antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Liver
CYP3A4 Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:18227-1-AP)