Calsequestrin 2 Polyclonal antibody

Calsequestrin 2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 27560-1-AP

Synonyms

Calsequestrin 2, CASQ2, PDIB2



Tested Applications

Positive WB detected inmouse heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27560-1-AP targets Calsequestrin 2 in WB, IP, IHC,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calsequestrin 2 fusion protein Ag26412
Full Name calsequestrin 2 (cardiac muscle)
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC022288
Gene symbol CASQ2
Gene ID (NCBI) 845
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calsequestrin 2 (CASQ2) specifies the cardiac muscle family member of the calsequestrin family. Calsequestrin is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. CASQ2 is an important component of calcium handling as a calcium storage protein in the sarcoplasmic reticulum (SR), playing an important role in the regulation of the cardiac ryanodine receptor (RyR2)-mediated calcium release and SR calcium release refractoriness. Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.

Protocols

Product Specific Protocols
WB protocol for Calsequestrin 2 antibody 27560-1-APDownload protocol
IHC protocol for Calsequestrin 2 antibody 27560-1-APDownload protocol
IP protocol for Calsequestrin 2 antibody 27560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols