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Recombinant Canine MCP-1/CCL2 protein (rFc Tag)

Species

Canine

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg4550

Validation Data Gallery

  • Purity of Recombinant Canine MCP-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Canine MCP-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Canine MCP-1 protein Gln24-Pro101 (Accession# P52203) with a rabbit IgG Fc tag at the C-terminus.
GeneID 403981
Accession P52203
PredictedSize 35.1 kDa
SDS-PAGE 36-45 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

MCP-1 (also known as CCL2) is a member of the chemokine family. MCP-1 recruits monocytes, memory T cells and dendritic cells to sites of inflammation caused by either tissue injury or infection. MCP-1 has been implicated in the pathogenesis of diseases characterised by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis. It binds to the chemokine receptors CCR2 and CCR4. Increased expression of MCP-1 is associated with severe acute respiratory syndrome coronavirus 2 infection.

References:

1.Singh S. et al. (2021) Int Immunopharmacol. 101(Pt B):107598. 
2.Lin Z. et al. (2023) Front Immunol. 13:1053457.
3.Li X. et al. (2017) Gut. 6(1):157-167.
4.Huang B. et al. (2007) Cancer Lett. 252(1):86-92.