Catalase Monoclonal antibody

Catalase Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (5)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F8D7

Cat no : 66765-1-Ig

Synonyms

CAT, catalase



Tested Applications

Positive WB detected inHeLa cells, rat brain tissue, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66765-1-Ig targets Catalase in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivity goat, human, mouse, pig, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Catalase fusion protein Ag16916
Full Name catalase
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC112219
Gene symbol CAT
Gene ID (NCBI) 847
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which also known as acatalasemia.

Protocols

Product Specific Protocols
WB protocol for Catalase antibody 66765-1-IgDownload protocol
IHC protocol for Catalase antibody 66765-1-IgDownload protocol
IF protocol for Catalase antibody 66765-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Haematologica

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Authors - Nunziata Maio

Sci China Life Sci

Cholesterol transport through the peroxisome-ER membrane contacts tethered by PI(4,5)P2 and extended synaptotagmins.

Authors - Jian Xiao
goatWB

Oxid Med Cell Longev

Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway.

Authors - Tian-Yu Feng
pigWB

Theriogenology

Melatonin ameliorates cypermethrin-induced impairments by regulating oxidative stress, DNA damage and apoptosis in porcine Sertoli cells.

Authors - Jing Li
ratIF

Eur J Pharmacol

PEX5 prevents cardiomyocyte hypertrophy via suppressing the redox-sensitive signaling pathways MAPKs and STAT3.

Authors - Minghui Wang
humanWB

Sci Rep

Repair Effects of Astragalus Polysaccharides with Different Molecular Weights on Oxidatively Damaged HK-2 Cells.

Authors - Jin Han