Catalase Monoclonal antibody

Catalase Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (5)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

16

CloneNo.

1F8D7

Cat no : 66765-1-Ig

Synonyms

CAT, catalase, Cell and organelle markers, Peroxisome Marker

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Validation Data Gallery

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  WB analysis using 66765-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66765-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66765-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66765-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 66765-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66765-1-Ig (Catalase antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 66765-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66765-1-Ig (Catalase antibody) at dilution of 1:500 (under 40x lens).

• 

  IF Staining of HepG2 using 66765-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Catalase antibody (66765-1-Ig, Clone: 1F8D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66765-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66765-1-Ig (Catalase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, rat brain tissue, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue
• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:100-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 13 publications below
• IF: See 3 publications below

Product Information

66765-1-Ig targets Catalase in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Cited Reactivity: human, mouse, rat, goat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Catalase fusion protein Ag16916
• Full Name: catalase
• Calculated molecular weight: 60 kDa
• Observed molecular weight: 60 kDa
• GenBank accession number: BC112219
• Gene symbol: CAT
• Gene ID (NCBI): 847
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which also known as acatalasemia.

Publications

Species	Application	Title
pig	WB	Microbiome; Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.; Authors - Leli Wang
mouse	WB	Biomaterials; MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.; Authors - Tian Wang
human	IF	Elife; Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stress; Authors - Jinyang Wang
goat	WB	Oxid Med Cell Longev; Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway.; Authors - Tian-Yu Feng
rat	WB	Oxid Med Cell Longev; Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis.; Authors - Honglin Liu
mouse	WB	Antioxidants (Basel); High-Intensity Interval Training and Moderate-Intensity Continuous Training Attenuate Oxidative Damage and Promote Myokine Response in the Skeletal Muscle of ApoE KO Mice on High-Fat Diet.; Authors - Linjia Wang