|Positive WB detected in||HeLa cells, rat brain tissue, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells, pig brain tissue|
|Positive IHC detected in||human hepatocirrhosis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66765-1-Ig targets Catalase in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
|Tested Reactivity||Human, Mouse, Rat, Pig|
|Cited Reactivity||human, mouse, rat, goat, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Catalase fusion protein Ag16916|
|Calculated molecular weight||60 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC112219|
|Gene ID (NCBI)||847|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which also known as acatalasemia.
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