p21 Polyclonal antibody

p21 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 28248-1-AP

Synonyms

CAP20, CDKI, Cdkn1, Cdkn1a, CIP1, mda6, P21, p21Cip1, p21WAF, SDI1, Waf1

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Validation Data Gallery

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  WB analysis using 28248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 28248-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 28248-1-AP (p21 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red).

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Tested Applications

• Positive WB detected in: PC-12 cells, NIH/3T3 cells, DC2.4 cells, HSC-T6 cells, RAW 264.7 cells, Sp2/0 cells, Neuro-2a cells
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

28248-1-AP targets p21 in WB, IHC, IF, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: p21 fusion protein Ag28394
• Full Name: cyclin-dependent kinase inhibitor 1A (P21)
• Calculated molecular weight: 18 kDa
• Observed molecular weight: 18 kDa
• GenBank accession number: NM_001111099
• Gene symbol: Cdkn1a
• Gene ID (NCBI): 12575
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Dev Cell; Endoderm development requires centrioles to restrain p53-mediated apoptosis in the absence of ERK activity.; Authors - Chang Xie
mouse	WB	Ecotoxicol Environ Saf; Exploring the molecular mechanisms underlie the endoplasmic reticulum stress-mediated methylmercury-induced neuronal developmental damage; Authors - Jingjing Pan
rat	WB	Front Mol Biosci; 6-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 Axis.; Authors - Jing Liu
mouse	WB, IHC	Hippocampus; NRSF deficiency leads to abnormal postnatal development of dentate gyrus and impairment of progenitors in subgranular zone of hippocampus.; Authors - Yan-Cong Wang
mouse,rat	IHC,IF	Int J Oral Sci; KLF6 facilitates differentiation of odontoblasts through modulating the expression of P21 in vitro.; Authors - Zhuo Chen
mouse	WB	Exp Ther Med; Sodium hydrosulfide alleviates dexamethasone-induced cell senescence and dysfunction through targeting the miR-22/sirt1 pathway in osteoblastic MC3T3-E1 cells.; Authors - Peng Li