p21 Polyclonal antibody
p21 Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 28248-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PC-12 cells, NIH/3T3 cells, DC2.4 cells, HSC-T6 cells, RAW 264.7 cells, Sp2/0 cells, Neuro-2a cells |
Positive IF detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 8 publications below |
IHC | See 3 publications below |
IF | See 2 publications below |
Product Information
28248-1-AP targets p21 in WB, IHC, IF, ELISA applications and shows reactivity with mouse, rat samples.
Tested Reactivity | mouse, rat |
Cited Reactivity | mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | p21 fusion protein Ag28394 |
Full Name | cyclin-dependent kinase inhibitor 1A (P21) |
Calculated molecular weight | 18 kDa |
Observed molecular weight | 18 kDa |
GenBank accession number | NM_001111099 |
Gene symbol | Cdkn1a |
Gene ID (NCBI) | 12575 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for p21 antibody 28248-1-AP | Download protocol |
IF protocol for p21 antibody 28248-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Dev Cell Endoderm development requires centrioles to restrain p53-mediated apoptosis in the absence of ERK activity. | ||
Ecotoxicol Environ Saf Exploring the molecular mechanisms underlie the endoplasmic reticulum stress-mediated methylmercury-induced neuronal developmental damage | ||
Front Mol Biosci 6-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 Axis. | ||
Hippocampus NRSF deficiency leads to abnormal postnatal development of dentate gyrus and impairment of progenitors in subgranular zone of hippocampus. | ||
Int J Oral Sci KLF6 facilitates differentiation of odontoblasts through modulating the expression of P21 in vitro. | ||
Exp Ther Med Sodium hydrosulfide alleviates dexamethasone-induced cell senescence and dysfunction through targeting the miR-22/sirt1 pathway in osteoblastic MC3T3-E1 cells. |