p21 Polyclonal antibody

p21 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28248-1-AP

Synonyms

CAP20, CDKI, Cdkn1, Cdkn1a, CIP1, mda6, P21, p21Cip1, p21WAF, SDI1, Waf1



Tested Applications

Positive WB detected inPC-12 cells, NIH/3T3 cells, DC2.4 cells, HSC-T6 cells, RAW 264.7 cells, Sp2/0 cells, Neuro-2a cells
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28248-1-AP targets p21 in WB, IHC, IF, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p21 fusion protein Ag28394
Full Name cyclin-dependent kinase inhibitor 1A (P21)
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberNM_001111099
Gene symbol Cdkn1a
Gene ID (NCBI) 12575
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for p21 antibody 28248-1-APDownload protocol
IF protocol for p21 antibody 28248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Exp Ther Med

Sodium hydrosulfide alleviates dexamethasone-induced cell senescence and dysfunction through targeting the miR-22/sirt1 pathway in osteoblastic MC3T3-E1 cells.

Authors - Peng Li
mouse,ratIHC,IF

Int J Oral Sci

KLF6 facilitates differentiation of odontoblasts through modulating the expression of P21 in vitro.

Authors - Zhuo Chen
mouseWB, IHC

Hippocampus

NRSF deficiency leads to abnormal postnatal development of dentate gyrus and impairment of progenitors in subgranular zone of hippocampus.

Authors - Yan-Cong Wang
ratWB

Front Mol Biosci

6-Shogaol Inhibits Oxidative Stress-Induced Rat Vascular Smooth Muscle Cell Apoptosis by Regulating OXR1-p53 Axis.

Authors - Jing Liu
mouseIF

Dev Cell

Endoderm development requires centrioles to restrain p53-mediated apoptosis in the absence of ERK activity.

Authors - Chang Xie