ChromoTek Cell Cycle Chromobody® plasmid (TagRFP)
DNA plasmid encoding for anti-PCNA VHH (anti-PCNA Nanobody) fused to fluorescent protein TagRFP. PCNA is a cell cycle marker.
Vector type
Mammalian expression vector
Promoter
Constitutive CMV IE
Reporter
TagRFP
Epitope
PCNA
Cat no : ccr
Synonyms
Validation Data Gallery
Product Information
DNA plasmid encoding for anti-PCNA VHH (anti-PCNA Nanobody) fused to fluorescent protein TagRFP. PCNA is a cell cycle marker.
Applications | Live cell imaging |
Specificity | PCNA (proliferating cell nuclear antigen), tested in human cells and zebrafish |
Vector type | Mammalian expression vector |
Promoter | Constitutive CMV IE |
Reporter | TagRFP |
Codon usage | Mammalian |
Selection | Kan/Neo |
Encoded Nanobody/ VHH | Monoclonal anti-PCNA single domain antibody (sdAb) fragment |
Sequence | With the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence. |
Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED and 3D-SIM; high-content microscopy and analysis |
Transfection | Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
Storage | Shipped at ambient temperature. Store at -20 °C |
Publications
Application | Title |
---|---|
Stem Cell Rev Rep Generation of Photoreceptor Precursors from Mouse Embryonic Stem Cells. | |
Sci Rep A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies. | |