ChromoTek Cell Cycle Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-PCNA VHH (anti-PCNA Nanobody) fused to fluorescent protein TagRFP. PCNA is a cell cycle marker.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagRFP

Epitope

PCNA

Cat no : ccr

Synonyms

Chromobody, PCNA Chromobody



Product Information

DNA plasmid encoding for anti-PCNA VHH (anti-PCNA Nanobody) fused to fluorescent protein TagRFP. PCNA is a cell cycle marker.

ApplicationsLive cell imaging
SpecificityPCNA (proliferating cell nuclear antigen), tested in human cells and zebrafish
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
ReporterTagRFP
Codon usageMammalian
SelectionKan/Neo
Encoded Nanobody/ VHHMonoclonal anti-PCNA single domain antibody (sdAb) fragment
SequenceWith the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED and 3D-SIM; high-content microscopy and analysis
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
StorageShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle
IF

Stem Cell Rev Rep

Generation of Photoreceptor Precursors from Mouse Embryonic Stem Cells.

Authors - Amy Q Lu

PLoS One

Quantitative live imaging of endogenous DNA replication in mammalian cells.

Authors - Andrew Burgess

Radiat Oncol

Live cell imaging at the Munich ion microbeam SNAKE - a status report.

Authors - Guido A Drexler

Sci Rep

A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies.

Authors - Bjoern Traenkle

Commun Biol

The γ-tubulin meshwork assists in the recruitment of PCNA to chromatin in mammalian cells.

Authors - Matthieu Corvaisier

Development

Live imaging of endogenous protein dynamics in zebrafish using chromobodies.

Authors - Paolo Panza