Cat no : ccr
DNA plasmid encoding for anti-PCNA VHH (anti-PCNA Nanobody) fused to fluorescent protein TagRFP. PCNA is a cell cycle marker.
|Applications||Live cell imaging|
|Specificity||PCNA (proliferating cell nuclear antigen), tested in human cells and zebrafish|
|Vector type||Mammalian expression vector|
|Promoter||Constitutive CMV IE|
|Encoded Nanobody/ VHH||Monoclonal anti-PCNA single domain antibody (sdAb) fragment|
|Sequence||With the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence.|
|Microscopy techniques||Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED and 3D-SIM; high-content microscopy and analysis|
|Transfection||Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.|
|Storage||Shipped at ambient temperature. Store at -20 °C|
Stem Cell Rev Rep
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