ChromoTek Cell Cycle Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-PCNA VHH (anti-PCNA Nanobody) fused to fluorescent protein TagRFP. PCNA is a cell cycle marker.




IF, Live cell imaging

Vector type

Mammalian expression vector



Cat no : ccr


Chromobody, PCNA Chromobody

Product Information

DNA plasmid encoding for anti-PCNA VHH (anti-PCNA Nanobody) fused to fluorescent protein TagRFP. PCNA is a cell cycle marker.

DescriptionThe Cell Cycle-Chromobody allows visualization of the endogenous cell cycle marker protein PCNA:

• Trace dynamic changes during the cell cycle in real-time

• Monitor the distribution of an endogenous cell cycle marker protein without artifacts or cytotoxic effects when overexpressing fluorescent fusion proteins

• Does not affect cellular functions

ApplicationsLive cell imaging
Visualizing endogenous cell cycle marker protein PCNA
Actin as biomarker or control
SpecificityPCNA (proliferating cell nuclear antigen), tested in human cells and zebrafish
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
Codon usageMammalian
SequenceWith the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence.
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED and 3D-SIM; high-content microscopy and analysis
Storage ConditionShipped at ambient temperature. Store at -20 °C




Equilibrium between nascent and parental MCM proteins protects replicating genomes.

Authors - Hana Sedlackova


CCNE1 amplification is synthetic lethal with PKMYT1 kinase inhibition.

Authors - David Gallo

Nat Cell Biol

Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress.

Authors - Noa Lamm

Nat Cell Biol

53BP1 nuclear bodies enforce replication timing at under-replicated DNA to limit heritable DNA damage.

Authors - Julian Spies

Mol Cell

DNA Replication Determines Timing of Mitosis by Restricting CDK1 and PLK1 Activation.

Authors - Bennie Lemmens


Evidence for the nuclear import of histones H3.1 and H4 as monomers.

Authors - Michael James Apta-Smith