ChromoTek Cell Cycle Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-PCNA VHH (anti-PCNA Nanobody) fused to fluorescent protein TagRFP. PCNA is a cell cycle marker.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagRFP

Epitope

PCNA

Publications

13

Cat no : ccr

Synonyms

Chromobody, PCNA Chromobody

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Validation Data Gallery

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  Live cell imaging of HeLa cells: Live cell imaging of HeLa cells stable expressing Cell Cycle-Chromobody (TagRFP). Images from a time series of 22 hours are shown

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  Cell Cycle-Chromobody signal during the cell cycle: In G1, the Chromobody signal is homogeneously distributed throughout the nucleus and cytoplasm. During S phase it accumulates in the nucleus and visualizes the formation of replication foci. In G2, the foci disappear and the cell divides (mitosis).

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  Time lapse video: Live cell imaging of the stable cell line HeLa-Cell Cycle Chromobody TagRFP expressing Cell Cycle-Chromobody.

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Product Information

DNA plasmid encoding for anti-PCNA VHH (anti-PCNA Nanobody) fused to fluorescent protein TagRFP. PCNA is a cell cycle marker.

• Applications: Live cell imaging
• Specificity: PCNA (proliferating cell nuclear antigen), tested in human cells and zebrafish
• Vector type: Mammalian expression vector
• Promoter: Constitutive CMV IE
• Reporter: TagRFP
• Codon usage: Mammalian
• Selection: Kan/Neo
• Encoded Nanobody/ VHH: Monoclonal anti-PCNA single domain antibody (sdAb) fragment
• Sequence: With the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED and 3D-SIM; high-content microscopy and analysis
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Storage: Shipped at ambient temperature. Store at -20 °C

Publications

Application	Title
IF	Stem Cell Rev Rep; Generation of Photoreceptor Precursors from Mouse Embryonic Stem Cells.; Authors - Amy Q Lu
	PLoS One; Quantitative live imaging of endogenous DNA replication in mammalian cells.; Authors - Andrew Burgess
	Radiat Oncol; Live cell imaging at the Munich ion microbeam SNAKE - a status report.; Authors - Guido A Drexler
	Sci Rep; A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies.; Authors - Bjoern Traenkle
	Commun Biol; The γ-tubulin meshwork assists in the recruitment of PCNA to chromatin in mammalian cells.; Authors - Matthieu Corvaisier
	Development; Live imaging of endogenous protein dynamics in zebrafish using chromobodies.; Authors - Paolo Panza