Cat no : PF00006
A distinctive feature of apoptosis is degradation of DNA. The DNA fragments tend to be vary in length in integer multiples of 180 bp-200 bp, which is shown as a ladder-like pattern in agarose gel electrophoresis. This kit uses the TUNEL method, using Terminal Deoxynucleotidyl Transferase (TdT) to catalyze the incorporation of CL488-dUTP at the 3'-OH end of the broken DNA of apoptotic cells. CL488-dUTP-labeled DNA can be directly observed with a fluorescence microscope or quantified with a flow cytometer. The TUNEL method can selectively detect apoptotic cells, but not necrotic cells or cells with DNA strand breaks caused by irradiation and drug treatment.
CL488 TUNEL Reaction Buffer
2 × 1.25 mL
Proteinase K (2 mg/mL)
DNase I (2 U/μL）
10 X DNase I Buffer
Note: Due to the upgrade and optimization of some TUNEL series products, the original component "TUNEL Equilibration Buffer" has been removed from some products, which will not affect the effect of the assay, and the step of incubation with equilibration solution can be directly omitted from the TUNEL reaction procedure.
Store at -20°C. Avoid exposure to light. Stable for 2 years after shipment. Avoid freeze- thaw cycles.
CoraLite® Plus 488 label, CL488 for short, this dye is similar to FITC and other dyes.
Cited in Article as
PF00006, CoraLite® Plus 488 TUNEL Assay Apoptosis Detection Kit (green), Proteintech, IL, USA
Oxid Med Cell Longev
ProNGF/NGF Modulates Autophagy and Apoptosis through PI3K/Akt/mTOR and ERK Signaling Pathways following Cerebral Ischemia-Reperfusion in Rats.
ACS Chem Neurosci
Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine.
ACS Biomater Sci Eng
siRNA Delivery with Stem Cell Membrane-Coated Magnetic Nanoparticles for Imaging-Guided Photothermal Therapy and Gene Therapy
Front Cell Infect Microbiol
Evidence of Infection of Human Embryonic Stem Cells by SARS-CoV-2.
Front Cell Neurosci
Healthy Serum-Derived Exosomes Improve Neurological Outcomes and Protect Blood-Brain Barrier by Inhibiting Endothelial Cell Apoptosis and Reversing Autophagy-Mediated Tight Junction Protein Reduction in Rat Stroke Model.
Adv Clin Exp Med
PIEZO2 promotes cell proliferation and metastasis in colon carcinoma through the SLIT2/ROBO1/VEGFC pathway