CoraLite®488 TUNEL Assay Apoptosis Detection Kit(green)

Excitation Laser

490 nm

Emission

515 nm

Application

FC

Cat no : PF00006



Product Information

A distinctive feature of apoptosis is degradation of DNA. The DNA fragments tend to be vary in length in integer multiples of 180 bp-200 bp, which is shown as a ladder-like pattern in agarose gel electrophoresis. This kit uses the TUNEL method, using Terminal Deoxynucleotidyl Transferase (TdT) to catalyze the incorporation of CL488-dUTP at the 3'-OH end of the broken DNA of apoptotic cells. CL488-dUTP-labeled DNA can be directly observed with a fluorescence microscope or quantified with a flow cytometer. The TUNEL method can selectively detect apoptotic cells, but not necrotic cells or cells with DNA strand breaks caused by irradiation and drug treatment.

 Components

20T

50T

 TUNEL Equilibration Buffer

2 X 1 mL

5 mL

 CL488 TUNEL Reaction Buffer

2 X 0.5 mL

5 X 0.5 mL

 TdT Enzyme

20 μL

50 μL

 Proteinase K (2 mg/mL)

40 μL

100 μL

 DNase I (2 U/μL)

5 μL

13 μL

 10 X DNase I Buffer

100 μL

260 μL


Storage

Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Avoid freeze- thaw cycles.

Conjugated

CoraLite®488 label, CL488 for short, this dye is similar to FITC and other dyes.

Ex/Em: 490/515nm