CoraLite®488 TUNEL Assay Apoptosis Detection Kit(green)

Excitation Laser

490 nm


515 nm



Cat no : PF00006

Product Information

A distinctive feature of apoptosis is degradation of DNA. The DNA fragments tend to be vary in length in integer multiples of 180 bp-200 bp, which is shown as a ladder-like pattern in agarose gel electrophoresis. This kit uses the TUNEL method, using Terminal Deoxynucleotidyl Transferase (TdT) to catalyze the incorporation of CL488-dUTP at the 3'-OH end of the broken DNA of apoptotic cells. CL488-dUTP-labeled DNA can be directly observed with a fluorescence microscope or quantified with a flow cytometer. The TUNEL method can selectively detect apoptotic cells, but not necrotic cells or cells with DNA strand breaks caused by irradiation and drug treatment.




 TUNEL Equilibration Buffer

2 X 1 mL

5 mL

 CL488 TUNEL Reaction Buffer

2 X 0.5 mL

5 X 0.5 mL

 TdT Enzyme

20 μL

50 μL

 Proteinase K (2 mg/mL)

40 μL

100 μL

 DNase I (2 U/μL)

5 μL

13 μL

 10 X DNase I Buffer

100 μL

260 μL


Store at -20°C. Avoid exposure to light. Stable for 2 years after shipment. Avoid freeze- thaw cycles.


CoraLite®488 label, CL488 for short, this dye is similar to FITC and other dyes.

Ex/Em: 490/515nm



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