Product Information
A distinctive feature of apoptosis is degradation of DNA. The DNA fragments tend to be vary in length in integer multiples of 180 bp-200 bp, which is shown as a ladder-like pattern in agarose gel electrophoresis. This kit uses the TUNEL method, using Terminal Deoxynucleotidyl Transferase (TdT) to catalyze the incorporation of CL488-dUTP at the 3'-OH end of the broken DNA of apoptotic cells. CL488-dUTP-labeled DNA can be directly observed with a fluorescence microscope or quantified with a flow cytometer. The TUNEL method can selectively detect apoptotic cells, but not necrotic cells or cells with DNA strand breaks caused by irradiation and drug treatment.
Components
Components | 20T | 50T |
CL488 TUNEL Reaction Buffer | 1 mL | 2 × 1.25 mL |
TdT Enzyme | 40 uL | 100 uL |
Proteinase K (2 mg/mL) | 40 uL | 100 uL |
DNase I (2 U/μL） | 5 uL | 13 uL |
10 X DNase I Buffer | 100 uL | 260 uL |
Note: Due to the upgrade and optimization of some TUNEL series products, the original component "TUNEL Equilibration Buffer" has been removed from some products, which will not affect the effect of the assay, and the step of incubation with equilibration solution can be directly omitted from the TUNEL reaction procedure.
Storage
Store at -20°C. Avoid exposure to light. Stable for 2 years after shipment. Avoid freeze- thaw cycles.
Conjugated
CoraLite® Plus 488 label, CL488 for short, this dye is similar to FITC and other dyes.
Ex/Em: 490/515nm
Cited in Article as
PF00006, CoraLite® Plus 488 TUNEL Assay Apoptosis Detection Kit, Proteintech, IL, USA
Publications
| Application | Title |
|---|---|
Adv Mater A Hierarchical Plug-and-Supply Flap (PASF) for Instant Perfusion and Oxygen Delivery in Large Soft Tissue Repair. | |
Nat Commun TCF21 promotes epithelial-to-mesenchymal transition and cytoskeleton reorganization in uterine development and endometriosis. | |
Nat Commun Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development | |
ACS Nano Single Atom Ru Doped CuTi Nanozyme with Precisely Programmed Cascade Catalysis for Amplified Oral Cancer Therapy. | |
J Nanobiotechnology Ethosomes-mediated tryptanthrin delivery as efficient anti-psoriatic nanotherapy by enhancing topical drug absorption and lipid homeostasis | |
Microbiome Sodium butyrate modulates ocular surface microbiome and attenuates inflammation of meibomian gland dysfunction in ApoE(-/-) mice. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Victoria (Verified Customer) (01-26-2026) | Coralite plus 488 TUNEL kit worked great in vivo. I would definitely recommend this reagent
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FH Arpita (Verified Customer) (07-17-2025) | This method is much quicker than the Click chemistry method.
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