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Cyclin A2 Monoclonal antibody

Cyclin A2 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a









Cat no : 66391-1-Ig


CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, Raji cells, Jurkat cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66391-1-Ig targets Cyclin A2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclin A2 fusion protein Ag12765
Full Name cyclin A2
Calculated molecular weight 432 aa, 49 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC104783
Gene symbol CCNA2
Gene ID (NCBI) 890
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.


Product Specific Protocols
WB protocol for Cyclin A2 antibody 66391-1-IgDownload protocol
IHC protocol for Cyclin A2 antibody 66391-1-IgDownload protocol
IF protocol for Cyclin A2 antibody 66391-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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