Cyclin A2 Monoclonal antibody

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  WB analysis using 66391-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66391-1-Ig

  WB result of Cyclin A2 antibody (66391-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cyclin A2 transfected HeLa cells.

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  IHC staining of human tonsillitis using 66391-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66391-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66391-1-Ig (Cyclin A2 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66391-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed HeLa cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of MCF-7 using 66391-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed MCF-7 cells using 66391-1-Ig(Cyclin A2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

• Featured Product
• KD/KO Validated

Cyclin A2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66391-1-Ig

Clone No.1C6C11

Publications(36)

Reviews (2)

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, Raji cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 28 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

66391-1-Ig targets Cyclin A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cyclin A2 fusion protein Ag12765
• Full Name: cyclin A2
• Calculated Molecular Weight: 432 aa, 49 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC104783
• Gene Symbol: Cyclin A2
• Gene ID (NCBI): 890
• RRID: AB_2881767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20248
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Dev Cell; Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.; Authors - Holly Merta
human	WB	Oncogene; KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition.; Authors - Y Zhong
human	IF	Elife; METTL3 promotes homologous recombination repair and modulates chemotherapeutic response in breast cancer by regulating the EGF/RAD51 axis.; Authors - Enjie Li
human	WB	J Cell Mol Med; Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma; Authors - Xiao-Han Cui
human	WB	Cell Cycle; Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D.; Authors - Jinghua Yu
human	WB	Molecules; A Potential Anti-Glioblastoma Compound LH20 Induces Apoptosis and Arrest of Human Glioblastoma Cells via CDK4/6 Inhibition; Authors - Yan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wissem (Verified Customer) (07-30-2024)

works well to detect cyclin A2 in extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

FH

Juri (Verified Customer) (01-20-2024)

Signal present after ICC, but expressing from all the cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 in 100
• Cell Tissue Type: cancer cells