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- KD/KO Validated
Cyclin A2 Monoclonal antibody
Cyclin A2 Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG2a
human and More (2)
WB, IHC, IF, ELISA
Cat no : 66391-1-Ig
|Positive WB detected in||HepG2 cells, HeLa cells, Raji cells, Jurkat cells, HEK-293 cells, HeLa cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66391-1-Ig targets Cyclin A2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Cyclin A2 fusion protein Ag12765|
|Full Name||cyclin A2|
|Calculated molecular weight||432 aa, 49 kDa|
|Observed molecular weight||52 kDa|
|GenBank accession number||BC104783|
|Gene ID (NCBI)||890|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
|Product Specific Protocols|
|WB protocol for Cyclin A2 antibody 66391-1-Ig||Download protocol|
|IHC protocol for Cyclin A2 antibody 66391-1-Ig||Download protocol|
|IF protocol for Cyclin A2 antibody 66391-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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