|Positive WB detected in
|HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, Raji cells
|Positive IHC detected in
|human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HeLa cells, MCF-7 cells
|Western Blot (WB)
|WB : 1:2000-1:10000
|IHC : 1:1000-1:4000
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66391-1-Ig targets Cyclin A2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|human, mouse, rat
|Host / Isotype
|Mouse / IgG2a
|Cyclin A2 fusion protein Ag12765
|Calculated molecular weight
|432 aa, 49 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Juri (Verified Customer) (01-20-2024)
Signal present after ICC, but expressing from all the cells.