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  • KD/KO Validated

Cyclin A2 Monoclonal antibody

Cyclin A2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1C6C11

Cat no : 66391-1-Ig

Synonyms

CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66391-1-Ig targets Cyclin A2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Cyclin A2 fusion protein Ag12765
Full Name cyclin A2
Calculated molecular weight 432 aa, 49 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC104783
Gene symbol CCNA2
Gene ID (NCBI) 890
RRIDAB_2881767
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Protocols

Product Specific Protocols
WB protocol for Cyclin A2 antibody 66391-1-IgDownload protocol
IHC protocol for Cyclin A2 antibody 66391-1-IgDownload protocol
IF protocol for Cyclin A2 antibody 66391-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Dev Cell

Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.

Authors - Holly Merta
humanWB

Oncogene

KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition.

Authors - Y Zhong
humanIF

Elife

METTL3 promotes homologous recombination repair and modulates chemotherapeutic response in breast cancer by regulating the EGF/RAD51 axis.

Authors - Enjie Li
humanWB

J Cell Mol Med

Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma

Authors - Xiao-Han Cui
humanWB

Cell Cycle

Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D.

Authors - Jinghua Yu
humanWB

Molecules

A Potential Anti-Glioblastoma Compound LH20 Induces Apoptosis and Arrest of Human Glioblastoma Cells via CDK4/6 Inhibition

Authors - Yan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Juri (Verified Customer) (01-20-2024)

Signal present after ICC, but expressing from all the cells.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1 in 100
  • Cell Tissue Type: cancer cells