Dystroglycan Monoclonal antibody

Dystroglycan Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (1)

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

CloneNo.

2B1G12

Cat no : 66735-1-Ig

Synonyms

156DAG, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG, DAG, DAG1, Dystroglycan



Tested Applications

Positive WB detected inA549 cells, mouse brain tissue, pig brain tissue, rat brain tissue, NCI-H1299 cells, HeLa cells, HepG2 cells
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

FCSee 1 publications below

Product Information

66735-1-Ig targets Dystroglycan in WB, IHC, FC,ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Dystroglycan fusion protein Ag27222
Full Name dystroglycan 1 (dystrophin-associated glycoprotein 1)
Calculated molecular weight 97 kDa
Observed molecular weight 43 kDa, 30 kDa
GenBank accession numberBC012740
Gene symbol DAG1
Gene ID (NCBI) 1605
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).

Protocols

Product Specific Protocols
WB protocol for Dystroglycan antibody 66735-1-IgDownload protocol
IHC protocol for Dystroglycan antibody 66735-1-IgDownload protocol
FC protocol for Dystroglycan antibody 66735-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Proc Natl Acad Sci U S A

CACNA1S haploinsufficiency confers resistance to New World arenavirus infection.

Authors - Nicolás Sarute