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DAXX Polyclonal antibody

DAXX Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20489-1-AP

Synonyms

BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using DAXX Polyclonal antibody (20489-1-AP)

    WB analysis of Y79 using 20489-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 20489-1-AP (DAXX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DAXX Polyclonal antibody (20489-1-AP)

    WB analysis of HeLa using 20489-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20489-1-AP (DAXX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inY79 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20489-1-AP is DAXX Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAXX fusion protein Ag14519
Full Name death-domain associated protein
Calculated molecular weight 740 aa, 81 kDa
Observed molecular weight 120 kDa, 70 kDa
GenBank accession numberBC109074
Gene symbol DAXX
Gene ID (NCBI) 1616
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Death associated protein 6 (DAXX) is a ubiquitous protein implicated in various cellular processes such as apoptosis, tumorigenesis, development and transcription. It has been shown to translocate from the nucleus to the cytoplasm under conditions of stress and to activate the Jun N-terminal kinase (JNK) pathway. Somatic mutations of death associated protein (DAXX) have been found in ALT cancers, including pancreatic neuroendocrine (panNET) cancers and glioblastoma multiforme (GBM) cancers.

Protocols

Product Specific Protocols
WB protocol for DAXX antibody 20489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen.

Authors - Alice Mac Kain
View Article
mouseWB

J Biol Chem

The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation.

Authors - Nasim A Begum
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Alexandros (Verified Customer) (03-03-2023)

Very happy with the final image we have in our blots compared to other antibodies we have previously used

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Adherent cells