DAXX Polyclonal antibody

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  WB analysis of Y79 using 20489-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20489-1-AP (DAXX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20489-1-AP (DAXX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 20489-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20489-1-AP (DAXX antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DAXX Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20489-1-AP

Publications(3)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

ETS1 associated protein 1, EAP1, Death domain-associated protein 6, Death domain associated protein 6, DAP6

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Tested Applications

• Positive WB detected in: Y79 cells, HeLa cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

20489-1-AP targets DAXX in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAXX fusion protein Ag14519
• Full Name: death-domain associated protein
• Calculated Molecular Weight: 740 aa, 81 kDa
• Observed Molecular Weight: 120 kDa, 70 kDa
• GenBank Accession Number: BC109074
• Gene Symbol: DAXX
• Gene ID (NCBI): 1616
• RRID: AB_10693620
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UER7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Death associated protein 6 (DAXX) is a ubiquitous protein implicated in various cellular processes such as apoptosis, tumorigenesis, development and transcription. It has been shown to translocate from the nucleus to the cytoplasm under conditions of stress and to activate the Jun N-terminal kinase (JNK) pathway. Somatic mutations of death associated protein (DAXX) have been found in ALT cancers, including pancreatic neuroendocrine (panNET) cancers and glioblastoma multiforme (GBM) cancers.

Publications

Species	Application	Title
human	IF	Nat Commun; Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen.; Authors - Alice Mac Kain
mouse	WB	J Biol Chem; The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation.; Authors - Nasim A Begum
		Sci Bull (Beijing); P53 upregulation by USP7-engaging molecular glues; Authors - Zhaoyang Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-13-2024)

The antibody strongly detected DAXX by western-blot (was used as a positive control in co-immunoprecipitation experiments) but also provided many other bands in HeLa cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

FH

Alexandros (Verified Customer) (03-03-2023)

Very happy with the final image we have in our blots compared to other antibodies we have previously used

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Adherent cells