DAXX Polyclonal antibody
DAXX Polyclonal Antibody for IF, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF,ELISA
Conjugate
Unconjugated
Cat no : 20489-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Y79 cells, HeLa cells |
Positive IF detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
Product Information
The immunogen of 20489-1-AP is DAXX Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DAXX fusion protein Ag14519 |
Full Name | death-domain associated protein |
Calculated molecular weight | 740 aa, 81 kDa |
Observed molecular weight | 120 kDa, 70 kDa |
GenBank accession number | BC109074 |
Gene symbol | DAXX |
Gene ID (NCBI) | 1616 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Death associated protein 6 (DAXX) is a ubiquitous protein implicated in various cellular processes such as apoptosis, tumorigenesis, developmentand transcription. It has been shown to translocate from the nucleus to the cytoplasm under conditions of stress and to activate the Jun N-terminal kinase (JNK) pathway. Somatic mutations of death associated protein (DAXX) have been found in ALT cancers, including pancreatic neuroendocrine (panNET) cancers and glioblastoma multiforme (GBM) cancers.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for DAXX antibody 20489-1-AP | Download protocol |
IF protocol for DAXX antibody 20489-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Biol Chem The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation. |