DAXX Polyclonal antibody

DAXX Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20489-1-AP

Synonyms

BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx



Tested Applications

Positive WB detected inY79 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20489-1-AP is DAXX Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAXX fusion protein Ag14519
Full Name death-domain associated protein
Calculated Molecular Weight 740 aa, 81 kDa
Observed Molecular Weight 120 kDa, 70 kDa
GenBank Accession NumberBC109074
Gene Symbol DAXX
Gene ID (NCBI) 1616
RRIDAB_10693620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Death associated protein 6 (DAXX) is a ubiquitous protein implicated in various cellular processes such as apoptosis, tumorigenesis, development and transcription. It has been shown to translocate from the nucleus to the cytoplasm under conditions of stress and to activate the Jun N-terminal kinase (JNK) pathway. Somatic mutations of death associated protein (DAXX) have been found in ALT cancers, including pancreatic neuroendocrine (panNET) cancers and glioblastoma multiforme (GBM) cancers.

Protocols

Product Specific Protocols
WB protocol for DAXX antibody 20489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen.

Authors - Alice Mac Kain
mouseWB

J Biol Chem

The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation.

Authors - Nasim A Begum

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Roy (Verified Customer) (06-13-2024)

The antibody strongly detected DAXX by western-blot (was used as a positive control in co-immunoprecipitation experiments) but also provided many other bands in HeLa cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa
FH

Alexandros (Verified Customer) (03-03-2023)

Very happy with the final image we have in our blots compared to other antibodies we have previously used

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Adherent cells