DAXX Polyclonal antibody

DAXX Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20489-1-AP

Synonyms

BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx

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Validation Data Gallery

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  WB analysis of Y79 using 20489-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20489-1-AP (DAXX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20489-1-AP (DAXX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 20489-1-AP

  Immunofluorescent analysis of A431 cells using 20489-1-AP (DAXX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Y79 cells, HeLa cells
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 20489-1-AP is DAXX Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAXX fusion protein Ag14519
• Full Name: death-domain associated protein
• Calculated molecular weight: 740 aa, 81 kDa
• Observed molecular weight: 120 kDa, 70 kDa
• GenBank accession number: BC109074
• Gene symbol: DAXX
• Gene ID (NCBI): 1616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Death associated protein 6 (DAXX) is a ubiquitous protein implicated in various cellular processes such as apoptosis, tumorigenesis, developmentand transcription. It has been shown to translocate from the nucleus to the cytoplasm under conditions of stress and to activate the Jun N-terminal kinase (JNK) pathway. Somatic mutations of death associated protein (DAXX) have been found in ALT cancers, including pancreatic neuroendocrine (panNET) cancers and glioblastoma multiforme (GBM) cancers.

Publications

Species	Application	Title
mouse	WB	J Biol Chem; The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation.; Authors - Nasim A Begum