Drebrin Polyclonal antibody

Drebrin Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10260-1-AP

Synonyms

D0S117E, DBN1, Drebrin, drebrin 1



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, Neuro-2a cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10260-1-AP targets Drebrin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Drebrin fusion protein Ag0371
Full Name drebrin 1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 100-130 kDa
GenBank Accession NumberBC000283
Gene Symbol DBN1
Gene ID (NCBI) 1627
RRIDAB_2230301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

Protocols

Product Specific Protocols
WB protocol for Drebrin antibody 10260-1-APDownload protocol
IHC protocol for Drebrin antibody 10260-1-APDownload protocol
IF protocol for Drebrin antibody 10260-1-APDownload protocol
IP protocol for Drebrin antibody 10260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

PLoS Genet

N-wasp is required for structural integrity of the blood-testis barrier.

Authors - Xiang Xiao
humanIF

Int J Mol Sci

Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery

Authors - Paloma Alvarez-Suarez
mouse,ratWB

Mol Neurobiol

L-3-n-Butylphthalide improves synaptic and dendritic spine plasticity and ameliorates neurite pathology in Alzheimer's disease mouse model and cultured hippocampal neurons.

Authors - Longjian Huang
mouseWB

Toxicol In Vitro

Bisphenol-A exposure induced neurotoxicity and associated with synapse and cytoskeleton in Neuro-2a cells.

Authors - Zhihong Yin
mouseWB

Environ Sci Pollut Res Int

Acute lead acetate induces neurotoxicity through decreased synaptic plasticity-related protein expression and disordered dendritic formation in nerve cells.

Authors - Lingli Chen
mouseWB

Gene

Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications.

Authors - Trieu-Duc Vu