|Positive WB detected in||HeLa cells, K-562 cells|
|Positive IP detected in||K-562 cells|
|Positive IF detected in||HeLa cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10260-1-AP targets Drebrin in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Drebrin fusion protein Ag0371|
|Full Name||drebrin 1|
|Calculated molecular weight||71 kDa|
|Observed molecular weight||100-130 kDa|
|GenBank accession number||BC000283|
|Gene ID (NCBI)||1627|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.
Cadmium induces actin cytoskeleton alterations and dysfunction in Neuro-2a cells.
Actin-binding protein drebrin E is involved in junction dynamics during spermatogenesis.
Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.
Contribution of single-minded 2 to hyperglycaemia-induced neurotoxicity.
Toxicol In Vitro
Bisphenol-A exposure induced neurotoxicity and associated with synapse and cytoskeleton in Neuro-2a cells.
L-3-n-Butylphthalide improves synaptic and dendritic spine plasticity and ameliorates neurite pathology in Alzheimer's disease mouse model and cultured hippocampal neurons.