Drebrin Polyclonal antibody

Drebrin Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10260-1-AP

Synonyms

D0S117E, DBN1, Drebrin, drebrin 1



Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inK-562 cells
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10260-1-AP targets Drebrin in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Drebrin fusion protein Ag0371
Full Name drebrin 1
Calculated molecular weight 71 kDa
Observed molecular weight 100-130 kDa
GenBank accession numberBC000283
Gene symbol DBN1
Gene ID (NCBI) 1627
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

Protocols

Product Specific Protocols
WB protocol for Drebrin antibody 10260-1-APDownload protocol
IF protocol for Drebrin antibody 10260-1-APDownload protocol
IP protocol for Drebrin antibody 10260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Environ Toxicol

Cadmium induces actin cytoskeleton alterations and dysfunction in Neuro-2a cells.

Authors - Yaming Ge
ratIHC,IF

Spermatogenesis

Actin-binding protein drebrin E is involved in junction dynamics during spermatogenesis.

Authors - Li Michelle Wm MW
mouseWB

Proteome Sci

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Authors - Lancia N F Darville
ratWB,IHC

Neurotoxicology

Contribution of single-minded 2 to hyperglycaemia-induced neurotoxicity.

Authors - Wang Xiaolan X
mouseWB

Toxicol In Vitro

Bisphenol-A exposure induced neurotoxicity and associated with synapse and cytoskeleton in Neuro-2a cells.

Authors - Zhihong Yin
mouse,ratWB

Mol Neurobiol

L-3-n-Butylphthalide improves synaptic and dendritic spine plasticity and ameliorates neurite pathology in Alzheimer's disease mouse model and cultured hippocampal neurons.

Authors - Longjian Huang